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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
puuB and puuE are separated by 37 nucleotides
puuE and pspF are separated by 119 nucleotides
pspF and pspA are separated by 166 nucleotides
pspA and pspB are separated by 53 nucleotides
b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536
puuB
b1302: puuE - GABA aminotransferase, PLP-dependent (NCBI), at 1,363,574 to 1,364,839
puuE
b1303: pspF - psp operon transcriptional activator (VIMSS), at 1,364,959 to 1,365,936
pspF
b1304: pspA - regulatory protein for phage-shock-protein operon (NCBI), at 1,366,103 to 1,366,771
pspA
b1305: pspB - phage shock protein B (NCBI), at 1,366,825 to 1,367,049
pspB
Group
Condition
b1301
b1302
b1303
b1304
b1305
puuB
puuE
pspF
pspA
pspB
nitrogen source
Putrescine (N)
-6.9
-0.3
-0.1
-0.6
-0.1
nitrogen source
Putrescine (N)
-5.5
-0.9
-0.1
-0.9
-0.3
stress
Bacitracin 1 mg/ml
-0.1
+0.3
-1.8
-0.7
-2.1
stress
toluene 2.5 vol%
-0.2
-0.1
-3.0
-0.5
-0.5
phage
Ste41
-0.5
-0.5
-0.9
-0.5
-1.5
control
PBS_cage_1_mice _Feces_22 hr
-0.4
-0.9
+0.3
-1.8
-0.3
stress
Aluminum chloride 5 mM
-0.0
+0.4
-1.1
-1.4
-0.8
stress
syringaldehyde 10 mM
-0.4
+0.2
-0.1
-1.2
-1.3
stress
Cisplatin 0.1 mg/ml
+0.1
+0.4
-0.2
-1.6
-1.4
carbon source
Glycolic Acid (C)
+0.2
-0.0
+0.0
-2.9
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.1
-0.4
+0.3
-2.5
+0.0
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.5
-0.0
-0.1
-2.4
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.2
+0.2
-0.2
-2.3
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
-0.9
+0.3
-0.8
-1.0
vitamin
M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM
+0.4
-0.1
-0.6
-1.5
-0.1
phage
RB68
-0.4
-0.6
-0.3
-1.3
+0.9
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
+0.2
+0.3
-1.9
-0.3
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.3
+0.9
-0.5
-1.2
-0.7
carbon source
Ying_Others16 rep B; time point 2
-0.5
-0.1
+0.1
+0.6
-1.3
control
LB
+0.3
+0.5
-0.2
-1.7
-0.0
carbon source
carbon source D-Glucose 0.25mM
+0.4
-0.8
-0.1
-0.8
+0.6
stress
octanol 0.08 vol%
+0.2
-0.5
+0.3
-1.7
+1.1
stress
LB Anaerobic with Sulfamonomethoxine 0.0000625 mM
-0.4
+0.3
-0.1
-1.1
+1.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
+1.3
-0.3
+0.4
-1.8
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.9
+0.9
+0.2
+0.5
-1.9
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.6
-0.3
+0.6
+1.2
-0.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.6
-0.6
+0.2
+1.4
+0.3
phage
N4_phage 0.009375 MOI TC2
+0.5
-0.4
+0.5
+1.2
+0.9
motility
outer cut, LB soft agar motility assay
+0.5
+1.1
+0.4
+0.0
+1.4
phage
Papa dilution 10-1 cacl2 maltose
+0.9
+0.7
+0.7
+1.6
-0.2
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