Fitness for 5 genes in Escherichia coli BW25113

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500 ntb1297 and ycjL are separated by 211 nucleotidesycjL and puuR are separated by 26 nucleotidespuuR and puuC are separated by 274 nucleotidespuuC and puuB are separated by 1 nucleotides b1297: b1297 - putative glutamine synthetase (EC 6.3.1.2) (VIMSS), at 1,357,514 to 1,358,932 b1297 b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908 ycjL b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492 puuR b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254 puuC b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536 puuB
Group Condition b1297 b1298 b1299 b1300 b1301
b1297 ycjL puuR puuC puuB
nitrogen source Putrescine (N) -4.9 -5.3 -4.4 -5.0 -6.9
nitrogen source Putrescine (N) -4.6 -3.8 -5.2 -5.3 -5.5
stress LB Aerobic with Irgasan 0.0000003125 mM -1.1 +0.5 -2.5 -0.0 -0.1
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.5 -1.1 -0.8 -0.4 -0.4
carbon source Ying_Nucleobase14 rep C; time point 3 -0.6 -0.9 -0.6 -0.4 -0.5
carbon source Ying_Nucleobase14 rep A; time point 3 -0.7 -0.6 -0.4 -0.5 -0.8
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.0 -1.4 -1.8 +0.8 -0.5
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.2 -1.4 +0.6 -0.9 -0.6
carbon source Ying_Nucleobase14 rep B; time point 3 -0.3 -0.7 -0.6 +0.0 -0.7
stress Cephalothin 0.01 mg/ml -0.2 -0.7 -1.1 +0.4 -0.5
control PBS_cage_1_mouse_1_Cecum_40 hr -0.0 -0.6 -1.5 +0.3 -0.2
msc 22hr_MSC_rep1_highinoculum +0.1 -1.0 -0.7 -0.4 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 -0.6 -1.1 -0.5 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -1.4 +0.5 -0.5 +0.2
control PBS_cage_1_mice _Feces_22 hr +0.1 +1.1 -1.2 -0.6 -0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -1.1 +0.4 -0.7 +0.9
stress Dimethyl Sulfoxide 10 vol% -0.7 +0.3 -0.9 +0.1 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -1.9 +0.1 +0.4 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -1.2 +0.9 -0.2 -0.0
stress Chloramphenicol 0.005 mg/ml -0.8 +0.4 +0.3 -0.7 +0.3
control PBS_cage_1_mice _Feces_16 hr +0.2 +1.1 -0.3 -0.4 -1.0
control PBS_cage_1_mice _Feces_40 hr +0.3 +0.8 -0.7 -0.4 -0.3
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.4 -0.6 +1.0 +0.3 -0.2
phage R1_N4_T2_phage8 +0.7 +0.7 -0.7 -0.4 -0.1
phage Ste41 +0.4 -1.0 +0.5 +0.2 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.6 +1.1 +0.2 -0.0
gfp_control GFP_control +0.5 +0.6 -0.3 +0.8 +0.1
phage R2_N4_T2_phage10 +0.5 -0.2 +0.5 +0.7 +0.4
carbon source carbon source D-Mannose 20mM +0.2 -0.3 +0.8 +0.7 +0.6
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.0 +0.6 +0.7 +0.2 +0.7
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