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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
sapC and sapB overlap by 14 nucleotides
sapB and sapA overlap by 4 nucleotides
sapA and ymjA are separated by 312 nucleotides
ymjA and ycjJ are separated by 133 nucleotides
b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542
sapC
b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494
sapB
b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134
sapA
b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692
ymjA
b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211
ycjJ
Group
Condition
b1292
b1293
b1294
b1295
b1296
sapC
sapB
sapA
ymjA
ycjJ
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-2.3
-6.5
-0.7
-0.2
-0.3
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-5.5
-3.8
-0.3
+0.1
-0.2
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-4.1
-5.5
-0.4
+0.0
+0.6
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
-4.6
-3.4
-0.2
-0.4
-0.2
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-3.8
-4.0
-0.4
-0.2
-0.3
nitrogen source
Putrescine (N)
-2.5
+0.0
+0.3
-1.4
-4.7
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
-2.8
-4.7
-0.4
-0.0
-0.2
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-4.4
-3.0
-0.4
-0.2
+0.1
carbon source
a-Ketoglutaric (C)
-3.5
-4.3
+0.2
-0.4
+0.5
carbon source
L-Fucose (C)
-3.2
-3.3
-0.0
-0.3
-0.0
stress
octanol 0.08 vol%
-1.8
-2.7
-0.9
-1.8
+0.4
phage
P2 dilution 10-3
-1.5
-4.3
-0.5
-0.6
+0.1
phage
Δdbn_10
-2.8
-3.4
-0.4
+0.3
-0.3
TN_15CS_15C_10dbl_rep1
-2.4
-4.0
-0.3
+0.3
+0.0
carbon source
L-Malic (C)
-4.0
-2.4
-0.0
-0.3
+0.5
carbon source
Ying_Nucleobase14 rep C; time point 2
-3.6
-3.5
+0.9
+0.2
-0.3
TN_18CS_18C_10dbl_rep1
-3.1
-3.4
+0.1
+0.2
+0.3
stress
Spectinomycin 0.00625 mg/ml
-3.4
-3.1
+0.3
-0.0
+0.6
nitrogen source
Putrescine (N)
-2.2
+0.4
+0.7
-0.1
-4.1
carbon source
Glycolic Acid (C)
-4.3
-1.8
+0.7
+0.3
+0.0
carbon source
carbon source D-Mannose 0.5mM
-1.4
-4.3
+0.4
+0.6
+0.2
stress
Benzalkonium Chloride 0.004 mg/ml
-2.2
-2.1
-1.0
-0.5
+1.6
stress
Spectinomycin 0.0125 mg/ml
-2.3
-2.6
+0.7
-1.0
+1.3
carbon source
Ying_Nucleobase14 rep B; time point 3
-4.2
-1.6
+1.3
+0.6
-0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.1
-3.0
-0.6
+1.2
+1.0
stress
Trimethoprim 0.005 mM
+2.2
+2.1
+1.7
-0.2
-1.0
stress
Nalidixic 0.06 mM
+3.1
+2.4
+1.2
-0.6
+0.1
stress
Cephalothin 0.01 mg/ml
+3.2
+2.6
+0.6
-0.3
+0.8
stress
2-Furfuraldehyde 0.25 vol%
+3.8
+3.9
+1.8
+0.2
+0.8
stress
Nalidixic 0.006 mg/ml
+5.7
+4.7
+1.6
-0.2
+0.2
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