Fitness for 5 genes in Escherichia coli BW25113

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500 ntoppD and oppF overlap by 4 nucleotidesoppF and b1248 are separated by 52 nucleotidesb1248 and cls are separated by 34 nucleotidescls and yciY are separated by 142 nucleotides b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791 oppD b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792 oppF b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174 b1248 b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669 cls b4595: yciY - hypothetical protein (RefSeq), at 1,306,812 to 1,306,985 yciY
Group Condition b1246 b1247 b1248 b1249 b4595
oppD oppF b1248 cls yciY
carbon source carbon source D-Glucose 0.25mM -0.1 -0.4 -6.2 -0.1 -2.3
motility outer cut, LB soft agar motility assay -0.2 -0.4 -2.9 -0.4 -2.2
carbon source carbon source D-Mannose 1mM -0.1 -0.4 -5.2 +0.3 -0.4
stress Benzalkonium Chloride 0.004 mg/ml +0.8 -0.6 +0.1 -3.2 -2.6
stress 3-methyl-2-butenol 1.25 vol% -0.1 -0.2 -0.5 -3.7 -0.0
phage Lambda_ci1857 -0.2 +0.3 -2.7 -0.7 -0.8
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.5 +0.7 -0.1 -3.6 -1.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.0 +0.8 +0.1 -3.6 -2.3
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.3 +0.3 -0.7 -4.5 +0.6
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 +0.8 +0.4 -4.1 -0.4
stress Cephalothin 0.01 mg/ml -0.6 -1.3 -0.3 +0.7 -1.6
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.1 +0.6 -1.3 -2.8 +0.6
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 -0.1 +0.9 -3.7 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +0.4 +1.1 -0.0 -4.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.1 +0.0 +0.2 -3.6 -0.1
stress Bacitracin 1 mg/ml -0.1 +0.5 -1.0 -1.7 +1.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.0 +0.6 -1.4 +2.6 -1.9
stress Cisplatin 0.1 mg/ml +1.2 +1.1 -0.8 -0.6 -0.7
stress 3-methyl-1-butanol 1.25 vol% -0.5 -0.4 -0.4 +3.1 -0.8
stress BHIS with Methotrexate 1.98 mM 24 hr +1.7 +2.0 -0.3 +0.4 +0.3
stress BHIS with Methotrexate 1.98 mM 24 hr +1.9 +2.0 -0.3 +0.2 +0.2
stress BHIS with Methotrexate 1.98 mM 24 hr +2.1 +2.0 -0.1 +0.2 -0.0
stress BHIS with Methotrexate 1.98 mM 48 hr +2.6 +3.0 -0.4 +0.0 +0.4
stress BHIS with Methotrexate 1.98 mM 48 hr +2.9 +3.0 -0.2 +0.1 +0.1
stress M9 with Methotrexate 1.98 mM 24 hr +3.7 +3.6 -0.2 -0.2 -0.3
stress BHIS with Methotrexate 1.98 mM 48 hr +3.4 +3.1 -0.2 +0.1 +0.4
stress M9 with Methotrexate 1.98 mM 48 hr +3.8 +3.6 -0.4 -0.3 +0.3
stress M9 with Methotrexate 1.98 mM 48 hr +5.4 +5.2 -0.3 -0.4 -0.6
stress M9 with Methotrexate 1.98 mM 24 hr +5.8 +5.7 -0.6 -0.0 -0.5
stress M9 with Methotrexate 1.98 mM 24 hr +10.0 +10.0 +0.0 -0.8 -0.2
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