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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
oppD and oppF overlap by 4 nucleotides
oppF and b1248 are separated by 52 nucleotides
b1248 and cls are separated by 34 nucleotides
cls and yciY are separated by 142 nucleotides
b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791
oppD
b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792
oppF
b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174
b1248
b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669
cls
b4595: yciY - hypothetical protein (RefSeq), at 1,306,812 to 1,306,985
yciY
Group
Condition
b1246
b1247
b1248
b1249
b4595
oppD
oppF
b1248
cls
yciY
carbon source
carbon source D-Glucose 0.25mM
-0.1
-0.4
-6.2
-0.1
-2.3
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
-2.9
-0.4
-2.2
carbon source
carbon source D-Mannose 1mM
-0.1
-0.4
-5.2
+0.3
-0.4
stress
Benzalkonium Chloride 0.004 mg/ml
+0.8
-0.6
+0.1
-3.2
-2.6
stress
3-methyl-2-butenol 1.25 vol%
-0.1
-0.2
-0.5
-3.7
-0.0
phage
Lambda_ci1857
-0.2
+0.3
-2.7
-0.7
-0.8
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.5
+0.7
-0.1
-3.6
-1.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+1.0
+0.8
+0.1
-3.6
-2.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
+0.3
-0.7
-4.5
+0.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.1
+0.8
+0.4
-4.1
-0.4
stress
Cephalothin 0.01 mg/ml
-0.6
-1.3
-0.3
+0.7
-1.6
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.1
+0.6
-1.3
-2.8
+0.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.3
-0.1
+0.9
-3.7
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
+0.4
+1.1
-0.0
-4.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+1.1
+0.0
+0.2
-3.6
-0.1
stress
Bacitracin 1 mg/ml
-0.1
+0.5
-1.0
-1.7
+1.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.0
+0.6
-1.4
+2.6
-1.9
stress
Cisplatin 0.1 mg/ml
+1.2
+1.1
-0.8
-0.6
-0.7
stress
3-methyl-1-butanol 1.25 vol%
-0.5
-0.4
-0.4
+3.1
-0.8
stress
BHIS with Methotrexate 1.98 mM 24 hr
+1.7
+2.0
-0.3
+0.4
+0.3
stress
BHIS with Methotrexate 1.98 mM 24 hr
+1.9
+2.0
-0.3
+0.2
+0.2
stress
BHIS with Methotrexate 1.98 mM 24 hr
+2.1
+2.0
-0.1
+0.2
-0.0
stress
BHIS with Methotrexate 1.98 mM 48 hr
+2.6
+3.0
-0.4
+0.0
+0.4
stress
BHIS with Methotrexate 1.98 mM 48 hr
+2.9
+3.0
-0.2
+0.1
+0.1
stress
M9 with Methotrexate 1.98 mM 24 hr
+3.7
+3.6
-0.2
-0.2
-0.3
stress
BHIS with Methotrexate 1.98 mM 48 hr
+3.4
+3.1
-0.2
+0.1
+0.4
stress
M9 with Methotrexate 1.98 mM 48 hr
+3.8
+3.6
-0.4
-0.3
+0.3
stress
M9 with Methotrexate 1.98 mM 48 hr
+5.4
+5.2
-0.3
-0.4
-0.6
stress
M9 with Methotrexate 1.98 mM 24 hr
+5.8
+5.7
-0.6
-0.0
-0.5
stress
M9 with Methotrexate 1.98 mM 24 hr
+10.0
+10.0
+0.0
-0.8
-0.2
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