Fitness for 5 genes in Escherichia coli BW25113

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500 ntrssA and rssB are separated by 91 nucleotidesrssB and galU are separated by 201 nucleotidesgalU and hns are separated by 143 nucleotideshns and tdk are separated by 604 nucleotides b1234: rssA - conserved protein (RefSeq), at 1,288,468 to 1,289,373 rssA b1235: rssB - response regulator of RpoS (NCBI), at 1,289,465 to 1,290,478 rssB b1236: galU - glucose-1-phosphate uridylyltransferase (NCBI), at 1,290,680 to 1,291,588 galU b1237: hns - global DNA-binding transcriptional dual regulator H-NS (NCBI), at 1,291,732 to 1,292,145 hns b1238: tdk - thymidine kinase (NCBI), at 1,292,750 to 1,293,367 tdk
Group Condition b1234 b1235 b1236 b1237 b1238
rssA rssB galU hns tdk
carbon source a-Ketoglutaric (C) -1.2 -5.6 -2.5 N.D. -0.8
carbon source acetate (C) -0.8 -4.7 -3.0 N.D. -0.5
carbon source a-Ketoglutaric (C) -1.2 -5.2 -2.1 N.D. -0.6
motility outer cut, LB soft agar motility assay -0.8 -1.0 -4.3 N.D. -2.2
control PBS_cage_2_mouse_1_Cecum_40 hr -0.3 +1.4 -5.8 N.D. -1.2
control PBS_cage_2_mice _Feces_40 hr +0.5 +1.4 -6.0 N.D. -1.0
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr +0.3 +1.5 -6.3 N.D. -0.3
phage Phage_N4_cage_3_mice _Feces_40 hr +0.6 +1.4 -5.9 N.D. -0.9
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr +0.4 +2.1 -6.4 N.D. -0.6
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr +0.3 +2.4 -6.4 N.D. -0.7
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr +0.2 +2.0 -6.2 N.D. -0.2
phage Phage_N4_cage_4_mice _Feces_40 hr +0.4 +1.7 -6.0 N.D. -0.3
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.6 +2.8 -6.1 N.D. -1.4
control PBS_cage_2_mouse_2_Cecum_40 hr +0.4 +2.2 -5.8 N.D. -0.6
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.6 +2.2 -5.6 N.D. -1.0
control PBS_cage_1_mouse_2_Cecum_40 hr +0.8 +1.8 -5.4 N.D. -0.9
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.9 +2.1 -5.2 N.D. -1.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +5.7 +1.4 N.D. -0.6
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.9 +2.8 +3.3 N.D. +1.6
phage Ste41 +1.0 +2.2 +5.7 N.D. +0.1
phage Ste41 +1.2 +2.3 +5.8 N.D. +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +2.3 +5.0 +1.7 N.D. +0.6
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.0 +1.6 +7.6 N.D. +0.6
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM +0.5 +0.6 +8.8 N.D. +0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.5 +4.0 +4.7 N.D. -0.2
stress Carbenicillin 0.008 mg/ml +1.5 +4.5 +3.1 N.D. +1.0
stress Cephalothin 0.01 mg/ml +0.6 +2.9 +4.8 N.D. +1.9
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.1 +1.1 +8.8 N.D. +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +2.2 +5.7 +5.7 N.D. -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.8 +5.9 +5.9 N.D. -0.1
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