Fitness for 5 genes in Escherichia coli BW25113

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500 ntnarL and narX overlap by 8 nucleotidesnarX and narK are separated by 338 nucleotidesnarK and narG are separated by 515 nucleotidesnarG and narH overlap by 4 nucleotides b1221: narL - DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI), at 1,274,402 to 1,275,052 narL b1222: narX - sensory histidine kinase in two-component regulatory system with NarL (NCBI), at 1,275,045 to 1,276,841 narX b1223: narK - nitrate/nitrite transporter (NCBI), at 1,277,180 to 1,278,571 narK b1224: narG - nitrate reductase 1, alpha subunit (NCBI), at 1,279,087 to 1,282,830 narG b1225: narH - nitrate reductase 1, beta (Fe-S) subunit (NCBI), at 1,282,827 to 1,284,365 narH
Group Condition b1221 b1222 b1223 b1224 b1225
narL narX narK narG narH
phage T7_phage 0.00015625 MOI TC3 -0.5 -1.1 -0.2 +0.0 -0.9
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.5 -0.0 -0.9 -0.9 -0.6
phage T7_phage 0.0015625 MOI TC2 +0.4 +0.3 -1.0 -0.4 -0.6
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.7 +0.0 -0.7 -0.8 -0.4
stress Aluminum chloride 5 mM +0.1 -1.4 +0.5 +0.3 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.9 +0.6 -0.6 -0.3 -0.3
carbon source L-Malic (C) +1.0 -0.4 +0.6 -0.2 -0.8
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 -0.4 -0.7 +0.6 +0.5
carbon source Glycolic Acid (C) +0.8 -0.5 +0.6 +0.2 -0.6
stress octanol 0.08 vol% +0.7 -1.2 +0.6 +0.1 +0.6
motility outer cut, LB soft agar motility assay +0.0 +1.0 +0.5 +0.5 -0.8
carbon source a-Ketoglutaric (C) +0.2 +1.4 +0.3 -0.0 -0.6
carbon source Ying_Others16 rep B; time point 1 +0.4 +1.3 +0.2 +0.1 -0.5
carbon source a-Ketoglutaric (C) +0.5 +1.4 +0.4 -0.2 -0.4
phage RB68 -3.3 +3.9 +0.5 +0.2 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.0 +1.1 -0.3 +0.0 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.3 +1.0 -0.5 -0.1 +0.3
stress Trimethoprim 0.005 mM +0.2 +1.3 +1.3 -0.3 -0.4
carbon source Ying_Others16 rep B; time point 2 -0.2 +2.8 -0.5 -0.0 +0.0
carbon source Adenosine 0.3742 mM (C) +0.2 +2.0 +0.3 +0.1 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.6 +0.2 +0.4 -0.2 +0.2
carbon source Guanosine 0.35306 mM (C) -0.2 +2.1 +0.1 +0.2 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 +0.7 -0.5 +1.0 +1.0
carbon source Ying_Others16 rep A; time point 2 +0.0 +3.1 -0.4 -0.1 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.4 +1.2 +1.0 +0.3 +0.1
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +1.5 -0.1 +0.4 +0.7 +0.8
phage RB68 -2.1 +4.1 +0.1 +0.7 +0.9
stress Chlorite 1.25 mM +0.4 -0.8 +5.1 -0.1 -0.0
phage Ste41 -1.3 +4.9 +0.0 +0.4 +1.0
phage Ste41 -0.3 +4.8 +0.3 +1.1 +1.0
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