Fitness for 5 genes in Escherichia coli BW25113

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500 ntldrC and rdlC are separated by 47 nucleotidesrdlC and chaA are separated by 288 nucleotideschaA and chaB are separated by 269 nucleotideschaB and chaC are separated by 157 nucleotides b4423: ldrC - toxic polypeptide, small (NCBI), at 1,269,461 to 1,269,568 ldrC b4424: rdlC - regulatory antisense sRNA (NCBI), at 1,269,616 to 1,269,683 rdlC b1216: chaA - calcium/sodium:proton antiporter (NCBI), at 1,269,972 to 1,271,072 chaA b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572 chaB b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425 chaC
Group Condition b4423 b4424 b1216 b1217 b1218
ldrC rdlC chaA chaB chaC
carbon source carbon source D-Glucose 0.25mM N.D. N.D. +0.2 -5.7 +0.2
carbon source carbon source D-Glucose 5mM N.D. N.D. +0.3 -5.4 -0.1
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) N.D. N.D. +0.2 -5.0 -0.5
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. -0.8 -4.3 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -0.0 -4.3 -0.1
phage R1_N4_T2_phage8 N.D. N.D. +0.3 -4.2 -0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. +0.5 -3.8 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -0.6 -2.7 -0.0
phage Bas60 N.D. N.D. -0.1 -3.4 +0.1
stress LB Anaerobic with Zinc Pyrithione 0.00000125 mM N.D. N.D. -0.1 -2.8 -0.4
protonated/deuterated deuterated glucose in H2O N.D. N.D. -0.0 -2.5 -0.5
stress Cisplatin 0.05 mg/ml N.D. N.D. -0.1 -2.5 -0.0
control PBS_cage_2_mouse_1_Cecum_40 hr N.D. N.D. +0.1 -2.7 -0.0
stress LB Anaerobic with Spectinomycin 0.0000625 mM N.D. N.D. +0.1 -2.0 -0.7
stress LB Anaerobic with Levofloxacin 0.00000003125 mM N.D. N.D. +0.0 -2.3 -0.1
stress Trimethoprim 0.0025 mM N.D. N.D. +0.1 -2.0 -0.5
phage Lambda_ci1857 N.D. N.D. -0.1 -2.4 +0.3
stress Cisplatin 0.025 mg/ml N.D. N.D. +0.3 -2.4 +0.0
stress acetate 70 mM N.D. N.D. +0.2 -2.3 +0.0
stress LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM N.D. N.D. -0.1 -2.2 +0.5
phage RB68 N.D. N.D. -0.1 -2.0 +0.6
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. +1.5 -2.4 +0.6
phage T7_phage 0.0015625 MOI TC2 N.D. N.D. -0.7 +1.6 -0.7
nophagecontrol R1_N4_T1_phage0 N.D. N.D. +0.3 +1.3 -1.0
phage N4_phage 0.009375 MOI TC2 N.D. N.D. -0.3 +2.0 -0.5
control LB N.D. N.D. -0.1 +2.2 +0.4
motility outer cut, LB soft agar motility assay N.D. N.D. +0.7 +1.2 +0.7
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. +1.5 +0.9 +0.4
stress 2-nitrobenzenesulfonyl fluoride N.D. N.D. -0.0 +3.0 +0.5
stress benzenesulfonyl fluoride N.D. N.D. -0.4 +3.2 +0.6
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