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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ldrC and rdlC are separated by 47 nucleotides
rdlC and chaA are separated by 288 nucleotides
chaA and chaB are separated by 269 nucleotides
chaB and chaC are separated by 157 nucleotides
b4423: ldrC - toxic polypeptide, small (NCBI), at 1,269,461 to 1,269,568
ldrC
b4424: rdlC - regulatory antisense sRNA (NCBI), at 1,269,616 to 1,269,683
rdlC
b1216: chaA - calcium/sodium:proton antiporter (NCBI), at 1,269,972 to 1,271,072
chaA
b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572
chaB
b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425
chaC
Group
Condition
b4423
b4424
b1216
b1217
b1218
ldrC
rdlC
chaA
chaB
chaC
carbon source
carbon source D-Glucose 0.25mM
N.D.
N.D.
+0.2
-5.7
+0.2
carbon source
carbon source D-Glucose 5mM
N.D.
N.D.
+0.3
-5.4
-0.1
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
N.D.
N.D.
+0.2
-5.0
-0.5
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
N.D.
N.D.
-0.8
-4.3
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
N.D.
-0.0
-4.3
-0.1
phage
R1_N4_T2_phage8
N.D.
N.D.
+0.3
-4.2
-0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
N.D.
N.D.
+0.5
-3.8
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
N.D.
-0.6
-2.7
-0.0
phage
Bas60
N.D.
N.D.
-0.1
-3.4
+0.1
stress
LB Anaerobic with Zinc Pyrithione 0.00000125 mM
N.D.
N.D.
-0.1
-2.8
-0.4
protonated/deuterated
deuterated glucose in H2O
N.D.
N.D.
-0.0
-2.5
-0.5
stress
Cisplatin 0.05 mg/ml
N.D.
N.D.
-0.1
-2.5
-0.0
control
PBS_cage_2_mouse_1_Cecum_40 hr
N.D.
N.D.
+0.1
-2.7
-0.0
stress
LB Anaerobic with Spectinomycin 0.0000625 mM
N.D.
N.D.
+0.1
-2.0
-0.7
stress
LB Anaerobic with Levofloxacin 0.00000003125 mM
N.D.
N.D.
+0.0
-2.3
-0.1
stress
Trimethoprim 0.0025 mM
N.D.
N.D.
+0.1
-2.0
-0.5
phage
Lambda_ci1857
N.D.
N.D.
-0.1
-2.4
+0.3
stress
Cisplatin 0.025 mg/ml
N.D.
N.D.
+0.3
-2.4
+0.0
stress
acetate 70 mM
N.D.
N.D.
+0.2
-2.3
+0.0
stress
LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
N.D.
N.D.
-0.1
-2.2
+0.5
phage
RB68
N.D.
N.D.
-0.1
-2.0
+0.6
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
+1.5
-2.4
+0.6
phage
T7_phage 0.0015625 MOI TC2
N.D.
N.D.
-0.7
+1.6
-0.7
nophagecontrol
R1_N4_T1_phage0
N.D.
N.D.
+0.3
+1.3
-1.0
phage
N4_phage 0.009375 MOI TC2
N.D.
N.D.
-0.3
+2.0
-0.5
control
LB
N.D.
N.D.
-0.1
+2.2
+0.4
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
+0.7
+1.2
+0.7
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
N.D.
N.D.
+1.5
+0.9
+0.4
stress
2-nitrobenzenesulfonyl fluoride
N.D.
N.D.
-0.0
+3.0
+0.5
stress
benzenesulfonyl fluoride
N.D.
N.D.
-0.4
+3.2
+0.6
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