Fitness for 5 genes in Escherichia coli BW25113

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500 ntnhaB and fadR are separated by 220 nucleotidesfadR and ycgB are separated by 51 nucleotidesycgB and dadA are separated by 329 nucleotidesdadA and dadX are separated by 9 nucleotides b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940 nhaB b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880 fadR b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data), at 1,234,932 to 1,236,464 ycgB b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data), at 1,236,794 to 1,238,092 dadA b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172 dadX
Group Condition b1186 b1187 b1188 b1189 b1190
nhaB fadR ycgB dadA dadX
motility outer cut, LB soft agar motility assay -1.0 -1.6 +0.3 -0.3 -2.0
nitrogen source D-Alanine (N) -0.0 +0.1 +0.0 -3.3 -1.4
mzaabcde mzaABCDE -0.6 -4.5 +0.7 +0.4 +0.0
gfp_control GFP_control -0.9 -3.7 +0.6 +0.2 -0.1
carbon source Ying_AminoAcid20 rep A; time point 3 -0.2 -2.6 +1.2 -0.4 -1.7
mzaabcde mzaABCDE -0.8 -3.3 +0.0 +0.6 -0.3
ppi ppI -0.6 -3.3 +0.4 +0.3 -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 -0.3 -2.5 +1.3 -0.2 -1.2
gfp_control GFP_control -0.4 -3.1 +0.3 +0.8 -0.2
control PBS_cage_1_mouse_1_Cecum_40 hr -0.2 -2.7 -0.1 +1.2 -0.7
carbon source Ying_AminoAcid20 rep B; time point 3 -0.1 -2.1 +1.4 +0.2 -1.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.2 -3.4 +0.7 +0.4 +0.5
carbon source Glycolic Acid (C) +0.4 -2.5 -0.7 +0.2 +1.0
carbon source L-Malic (C) +0.6 -3.1 -0.2 +0.4 +0.9
carbon source Glycolic Acid (C) +0.4 -2.3 -0.7 +0.3 +1.1
carbon source Ying_Nucleobase14 rep C; time point 2 +0.3 +0.9 -2.9 +0.4 +2.2
carbon source Ying_Nucleobase14 rep B; time point 2 +0.1 +0.9 -2.9 +0.5 +2.4
carbon source Ying_Nucleobase14 rep A; time point 2 +0.3 +0.9 -2.5 +0.3 +2.3
carbon source Ying_Nucleobase14 rep A; time point 3 +0.2 +1.3 -3.5 +0.7 +3.1
carbon source Ying_Nucleobase14 rep C; time point 3 +0.3 +1.1 -2.9 +0.5 +3.0
carbon source Ying_Nucleobase14 rep B; time point 3 +0.1 +1.3 -2.9 +0.7 +3.2
stress Chloramphenicol 0.0025 mg/ml -0.6 +3.6 +0.3 -0.1 +0.4
nitrogen source L-Arginine (N) +0.2 +0.1 +4.5 -0.2 +0.3
nitrogen source L-Arginine (N) +0.2 +0.1 +4.6 +0.1 +0.1
carbon source Acetate (C) +0.1 +2.3 +1.9 +0.2 +0.8
carbon source Acetate (C) +0.6 +2.2 +1.8 +0.1 +0.8
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.8 +6.3 -0.4 +0.4 +0.2
stress LB Aerobic with Novobiocin 0.0000625 mM +0.4 +5.1 +0.4 -0.2 +0.4
stress LB Aerobic with Novobiocin 0.0000625 mM +0.4 +5.6 +0.5 -0.0 +0.2
stress LB Aerobic with Irgasan 0.0000003125 mM -0.0 +8.0 -0.0 +0.1 +0.3
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