Fitness for 5 genes in Escherichia coli BW25113

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500 ntycgN and hlyE are separated by 206 nucleotideshlyE and umuD are separated by 372 nucleotidesumuD and umuC overlap by 1 nucleotidesumuC and dsbB are separated by 45 nucleotides b1181: ycgN - conserved protein (RefSeq), at 1,228,038 to 1,228,499 ycgN b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617 hlyE b1183: umuD - DNA polymerase V, subunit D (NCBI), at 1,229,990 to 1,230,409 umuD b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677 umuC b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253 dsbB
Group Condition b1181 b1182 b1183 b1184 b1185
ycgN hlyE umuD umuC dsbB
stress Cisplatin 0.1 mg/ml -0.5 -2.1 -1.4 -3.3 -2.0
carbon source carbon source D-Mannose 0.5mM -0.3 -0.2 -4.8 +0.2 -3.7
stress Cisplatin 0.1 mg/ml -0.8 -0.5 -2.4 -3.5 -1.0
carbon source carbon source D-Glucose 0.25mM -0.0 -0.2 -4.9 +0.1 -2.6
stress Cisplatin 0.1 mg/ml -0.5 -0.4 -2.9 -2.5 -0.7
M9 Glucose 2% aerobic -0.0 -5.7 -0.1 +0.1 -0.6
carbon source Ying_sugar7 rep A; time point 3 +0.2 -1.5 -0.4 -0.1 -4.6
carbon source Ying_sugar7 rep C; time point 3 -0.0 -1.5 -0.6 -0.1 -3.4
stress Cisplatin 0.1 mg/ml -0.7 -0.3 -0.7 -2.5 -1.3
carbon source carbon source D-Mannose 5mM +0.4 +0.2 -4.6 +0.5 -1.7
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.2 +0.5 -2.6 -0.0 -2.7
stress M9 with Methotrexate 0.06 mM 24 hr -0.0 -0.3 -0.8 +0.0 -3.8
motility outer cut, LB soft agar motility assay +0.2 -1.9 -1.0 +0.1 -2.0
sir2-hera SIR2-HerA +0.3 -0.7 -0.6 +0.4 -3.9
ppi ppI +0.2 -1.0 -0.7 +0.1 -3.2
stress LB Aerobic with Irgasan 0.0000003125 mM -0.7 +0.3 -0.9 -0.4 -2.6
phage N4_phage 0.00009375 MOI TC5 +0.2 +0.5 -2.5 -0.2 -1.9
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.1 +1.0 +1.5 +0.0 -5.4
phage N4_phage 0.009375 MOI TC2 -0.9 +2.9 -0.4 +0.2 -1.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.2 +0.0 -2.0 -0.2 +4.4
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.1 +0.1 -1.1 -0.3 +3.8
carbon source Ying_Others16 rep B; time point 2 -0.5 -0.7 +0.4 +0.4 +3.4
carbon source Ying_Others16 rep A; time point 2 -0.2 -0.7 +0.3 -0.1 +3.8
phage Ste41 -0.4 +1.5 +1.8 -1.0 +1.5
stress Trimethoprim 0.0025 mM +0.0 +0.7 -0.9 -0.0 +4.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 +0.2 +0.1 -0.4 +5.0
phage Ste41 +0.3 +1.5 +1.2 +0.1 +2.0
stress Trimethoprim 0.005 mM +0.8 +2.5 -1.3 +0.2 +3.4
stress Carbenicillin 0.008 mg/ml +0.1 -0.1 +0.3 -0.2 +5.8
stress Cephalothin 0.01 mg/ml +0.3 +0.9 -0.5 -0.1 +5.3
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