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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ycfN and nagZ are separated by 10 nucleotides
nagZ and ycfP are separated by 22 nucleotides
ycfP and ndh are separated by 399 nucleotides
ndh and ycfJ are separated by 209 nucleotides
b1106: ycfN - thiamin kinase (NCBI), at 1,162,483 to 1,163,307
ycfN
b1107: nagZ - beta-hexosaminidase (NCBI), at 1,163,318 to 1,164,343
nagZ
b1108: ycfP - orf, hypothetical protein (VIMSS), at 1,164,366 to 1,164,908
ycfP
b1109: ndh - respiratory NADH dehydrogenase 2/cupric reductase (NCBI), at 1,165,308 to 1,166,612
ndh
b1110: ycfJ - hypothetical protein (NCBI), at 1,166,822 to 1,167,361
ycfJ
Group
Condition
b1106
b1107
b1108
b1109
b1110
ycfN
nagZ
ycfP
ndh
ycfJ
phage
HSDP4
-0.8
-0.9
-1.4
-2.6
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.7
-2.2
-0.9
-1.7
+0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.5
-2.3
-0.6
-1.7
-0.1
stress
Cephalothin 0.01 mg/ml
-0.1
-1.8
-0.4
-2.3
-0.0
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.1
-0.4
-2.1
-1.3
-0.1
msc
3hr_mediaonly_rep2_lowinoculum
+0.1
+0.1
-0.9
-2.7
-0.1
mzaabcde
mzaABCDE
-0.6
-0.4
-0.3
-2.8
+0.6
stress
3-methyl-2-butenol 1.25 vol%
-0.2
-0.0
+0.5
-3.6
-0.1
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.1
-0.5
-0.1
-2.8
+0.1
gfp_control
GFP_control
-0.5
-0.4
-0.3
-2.5
+0.5
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.3
-0.3
-2.0
-0.6
+0.2
stress
3-methyl-2-butenol 1.25 vol%
+0.1
+0.0
-0.5
-2.8
+0.1
mzaabcde
mzaABCDE
-0.7
-0.5
+0.1
-2.4
+0.6
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.0
-2.8
+0.1
-0.5
+0.4
retron_ec_48_control
retron_Ec_48_control
-0.1
-0.1
-0.2
-2.6
+0.7
ppi
ppI
-0.5
-0.1
+0.5
-3.1
+0.6
mzaabcde
mzaABCDE
-0.7
-0.5
+0.2
-1.8
+0.6
phage
TP4
+0.5
+0.3
-0.8
-1.2
-0.8
gfp_control
GFP_control
-0.4
-0.1
-0.5
-1.9
+0.8
stress
octanol 0.08 vol%
-0.9
-0.4
+0.3
-1.8
+0.6
drt_type_3
DRT_type_3
-0.3
-0.1
+0.4
-2.6
+0.7
phage
NpK
+0.1
+0.4
-0.9
-1.8
+0.5
msc
22hr_MSC_rep1_highinoculum
+0.7
+0.4
-2.2
-0.0
+0.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.6
-0.2
+1.4
+0.4
-1.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.1
+0.2
-2.1
+1.8
+0.3
carbon source
carbon source D-Glucose 0.25mM
-0.6
+0.4
+1.9
-0.4
-0.3
carbon source
carbon source D-Glucose 5mM
-0.3
+0.9
+1.6
-0.7
+0.2
motility
outer cut, LB soft agar motility assay
+0.5
-0.2
+1.0
+1.1
-0.8
control
PBS_cage_1_mice _Feces_16 hr
+0.3
-0.5
+1.8
+0.5
-0.3
stress
2-Furfuraldehyde 0.25 vol%
-0.2
+0.3
-0.4
+3.5
-0.1
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