Fitness for 5 genes in Escherichia coli BW25113

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500 ntyceD and rpmF are separated by 51 nucleotidesrpmF and plsX are separated by 80 nucleotidesplsX and fabH are separated by 67 nucleotidesfabH and fabD are separated by 15 nucleotides b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538 yceD b1089: rpmF - 50S ribosomal protein L32 (NCBI), at 1,146,590 to 1,146,763 rpmF b1090: plsX - glycerolphosphate auxotrophy in plsB background (VIMSS), at 1,146,844 to 1,147,914 plsX b1091: fabH - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,147,982 to 1,148,935 fabH b1092: fabD - acyl carrier protein S-malonyltransferase (NCBI), at 1,148,951 to 1,149,880 fabD
Group Condition b1088 b1089 b1090 b1091 b1092
yceD rpmF plsX fabH fabD
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.3 N.D. -5.0 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -2.3 N.D. -1.3 N.D. N.D.
motility outer cut, LB soft agar motility assay -2.0 N.D. -1.5 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -1.7 N.D. -1.1 N.D. N.D.
stress Nickel (II) chloride 1 mM -1.1 N.D. -1.5 N.D. N.D.
phage HSDP4 -2.8 N.D. +0.3 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -1.5 N.D. -1.0 N.D. N.D.
stress Cephalothin 0.01 mg/ml -2.1 N.D. -0.1 N.D. N.D.
stress undecene 5 vol% -0.3 N.D. -2.0 N.D. N.D.
sir2-hera SIR2-HerA -0.8 N.D. -1.4 N.D. N.D.
motility outer cut, LB soft agar motility assay -2.2 N.D. +0.5 N.D. N.D.
yeast Calbicans +0.5 N.D. -1.9 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.4 N.D. -2.2 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.4 N.D. -2.0 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.9 N.D. -2.1 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.5 N.D. -1.6 N.D. N.D.
stress Tetracycline 0.0008 mg/ml -1.0 N.D. +1.5 N.D. N.D.
phage T7_phage 0.0015625 MOI TC2 -1.2 N.D. +1.7 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.8 N.D. -0.8 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +2.2 N.D. -1.0 N.D. N.D.
stress octanol 0.08 vol% -0.9 N.D. +2.4 N.D. N.D.
stress Chloramphenicol 0.0025 mg/ml -0.6 N.D. +2.3 N.D. N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.5 N.D. +2.1 N.D. N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM -0.3 N.D. +2.0 N.D. N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM -0.4 N.D. +2.5 N.D. N.D.
plasmid transformation mariner vector transformation; agar assay +0.8 N.D. +1.5 N.D. N.D.
carbon source Acetate (C) +1.3 N.D. +1.1 N.D. N.D.
plasmid transformation vcDART vector transformation; agar assay +1.3 N.D. +1.1 N.D. N.D.
plasmid transformation vcDART vector transformation; agar assay +1.1 N.D. +1.4 N.D. N.D.
carbon source Acetate (C) +1.2 N.D. +1.3 N.D. N.D.
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