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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ycdQ and ycdR overlap by 8 nucleotides
ycdR and ycdS are separated by 8 nucleotides
ycdS and ycdT are separated by 586 nucleotides
ycdT and insF-4 are separated by 40 nucleotides
b1022: ycdQ - predicted glycosyl transferase (NCBI), at 1,085,744 to 1,087,069
ycdQ
b1023: ycdR - predicted enzyme associated with biofilm formation (NCBI), at 1,087,062 to 1,089,080
ycdR
b1024: ycdS - predicted outer membrane protein (NCBI), at 1,089,089 to 1,091,512
ycdS
b1025: ycdT - predicted diguanylate cyclase (NCBI), at 1,092,099 to 1,093,457
ycdT
b1026: insF-4 - IS3 element protein InsF (NCBI), at 1,093,498 to 1,094,364
insF-4
Group
Condition
b1022
b1023
b1024
b1025
b1026
ycdQ
ycdR
ycdS
ycdT
insF-4
phage
Phage_N4_cage_3_mice _Feces_40 hr
+0.1
-0.0
+0.1
-3.7
N.D.
M9 Acetate 0.5% aerobic
-0.5
-0.8
-0.5
-1.4
N.D.
M9 Acetate 0.5% aerobic
-0.4
-0.7
-0.5
-1.5
N.D.
carbon source
carbon source D-Glucose 0.25mM
-0.3
-0.3
+0.1
-2.7
N.D.
predation
3 hr rep1_Tiberius_no_dilution
-0.4
-0.3
-0.2
-2.1
N.D.
M9 Glucose 2%, 20 mM NO3- anaerobic
-0.5
-0.8
-0.4
-1.1
N.D.
M9 Glucose 2% anaerobic
-0.5
-0.7
-0.3
-1.3
N.D.
phage
TP4
-0.2
-0.3
-0.3
-1.9
N.D.
mzaabcde
mzaABCDE
+0.1
-0.6
-0.3
-2.0
N.D.
nophagecontrol
NoPhageControl
-0.3
-0.2
+0.0
-2.2
N.D.
M9 Glucose 2% aerobic
-0.5
-0.6
-0.3
-1.1
N.D.
phage
Phage_N4_cage_3_mice _Feces_22 hr
+0.0
-0.2
+0.1
-2.6
N.D.
phage
Bas83
-0.2
-0.1
+0.0
-2.4
N.D.
phage
T7_phage 0.00015625 MOI TC3
-0.3
-0.3
+0.0
-2.0
N.D.
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
+0.0
-0.2
+0.2
-2.6
N.D.
predation
18 hr rep1_Tiberius_10fold_dilution
-0.0
-0.2
-0.2
-2.2
N.D.
phage
Phage_N4_cage_3_mouse_2_Cecum_40 hr
+0.3
-0.0
+0.0
-2.9
N.D.
M9 Glucose 2% aerobic
-0.4
-0.6
-0.3
-1.3
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
+0.2
-0.3
-2.1
N.D.
predation
3 hr rep1_HD100_no_dilution
-0.2
-0.2
-0.3
-1.7
N.D.
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.1
-0.2
+0.1
-2.3
N.D.
phage
Goslar
-0.2
-0.3
+0.1
-1.8
N.D.
predation
48 hr rep1_HD100_no_dilution
+0.0
+0.2
+0.1
-2.3
N.D.
msc
6hr_mediaonly_rep1_lowinoculum
+0.1
-0.1
+0.3
-2.3
N.D.
phage
Mpec16
+0.0
+0.1
+0.2
-2.3
N.D.
msc
22hr_mediaonly_rep1_lowinoculum
+0.2
-0.0
+0.1
-2.2
N.D.
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.5
+0.2
+0.3
-1.9
N.D.
carbon source
carbon source D-Mannose 0.5mM
-0.6
-0.3
-0.6
+1.2
N.D.
phage
N4_phage 0.009375 MOI TC2
+0.3
+0.9
+0.3
+0.8
N.D.
stress
Trimethoprim 0.005 mM
+0.6
+0.8
+0.2
+1.2
N.D.
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