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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ycdC and putA are separated by 39 nucleotides
putA and putP are separated by 422 nucleotides
putP and efeU are separated by 542 nucleotides
efeU and ycdO are separated by 57 nucleotides
b1013: ycdC - predicted DNA-binding transcriptional regulator (NCBI), at 1,073,465 to 1,074,103
ycdC
b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105
putA
b1015: putP - proline:sodium symporter (NCBI), at 1,078,528 to 1,080,036
putP
b4490: efeU - no description, at 1,080,579 to 1,081,408
efeU
b1018: ycdO - hypothetical protein (NCBI), at 1,081,466 to 1,082,593
ycdO
Group
Condition
b1013
b1014
b1015
b4490
b1018
ycdC
putA
putP
efeU
ycdO
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.7
-0.1
-0.3
+0.2
-0.2
carbon source
Ying_AminoAcid20 rep A; time point 3
+0.5
-1.3
-1.4
+0.2
-0.0
carbon source
Ying_AminoAcid20 rep A; time point 2
+0.1
-0.9
-1.1
-0.1
+0.0
carbon source
Ying_AminoAcid20 rep C; time point 3
+0.4
-1.2
-1.2
+0.0
-0.0
carbon source
Ying_AminoAcid20 rep B; time point 3
+0.2
-1.2
-1.0
+0.2
+0.1
carbon source
carbon source D-Glucose 0.25mM
-2.4
+0.2
+0.1
+0.2
+0.1
phage
T7_phage 0.00015625 MOI TC4
-1.2
+0.4
-0.3
-0.3
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.4
-0.3
+0.2
+0.0
+0.2
carbon source
Ying_AminoAcid20 rep C; time point 2
+0.6
-0.9
-0.8
-0.0
+0.0
carbon source
casaminos (C)
+0.6
-0.8
-0.7
-0.1
-0.1
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.4
-0.3
-0.9
-0.6
+0.4
stress
Chloramphenicol 0.005 mg/ml
-1.6
+0.1
+0.2
+0.2
+0.1
stress
LB Aerobic with Oxytetracycline 0.00000125 mM
-0.9
-0.1
+0.1
+0.5
-0.3
carbon source
casaminos (C)
+0.6
-0.6
-0.6
-0.1
+0.2
phage
Ste41
-1.7
+0.4
-0.1
+0.8
+0.3
stress
LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
-0.8
+0.2
-0.2
+0.3
+0.4
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-1.1
+0.2
+0.7
+0.3
-0.1
phage
Ste41
-1.4
+0.3
-0.1
+1.5
-0.1
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+1.2
-0.1
-0.2
+0.1
-0.7
phage
T7_phage 0.0015625 MOI TC2
+1.1
+0.0
-0.7
+0.5
-0.3
phage
Δdbn_10
+1.5
-0.3
+0.0
-0.3
-0.2
carbon source
carbon source D-Mannose 5mM
+0.3
+0.5
+0.4
-0.6
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+1.3
-0.4
+0.0
-0.2
+0.2
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+1.4
-0.0
-0.3
+0.2
+0.1
motility
outer cut, LB soft agar motility assay
+1.0
-0.4
-0.2
+0.9
+0.0
stress
LB Anaerobic with Trimethoprim 0.0000003125 mM
+0.9
+0.1
-0.2
+0.4
+0.4
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+1.0
-0.1
-0.1
+0.6
+0.3
control
PBS_cage_1_mice _Feces_16 hr
+1.2
-0.2
+0.3
-0.0
+0.6
control
PBS_cage_2_mouse_2_Cecum_40 hr
+1.4
+0.1
-0.1
+0.5
+0.2
phage
Papa dilution 10-1 cacl2 maltose
+1.5
+0.4
+0.4
+0.8
-0.3
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