Fitness for 5 genes in Escherichia coli BW25113

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500 ntycdC and putA are separated by 39 nucleotidesputA and putP are separated by 422 nucleotidesputP and efeU are separated by 542 nucleotidesefeU and ycdO are separated by 57 nucleotides b1013: ycdC - predicted DNA-binding transcriptional regulator (NCBI), at 1,073,465 to 1,074,103 ycdC b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105 putA b1015: putP - proline:sodium symporter (NCBI), at 1,078,528 to 1,080,036 putP b4490: efeU - no description, at 1,080,579 to 1,081,408 efeU b1018: ycdO - hypothetical protein (NCBI), at 1,081,466 to 1,082,593 ycdO
Group Condition b1013 b1014 b1015 b4490 b1018
ycdC putA putP efeU ycdO
stress LB Aerobic with Irgasan 0.0000003125 mM -1.7 -0.1 -0.3 +0.2 -0.2
carbon source Ying_AminoAcid20 rep A; time point 3 +0.5 -1.3 -1.4 +0.2 -0.0
carbon source Ying_AminoAcid20 rep A; time point 2 +0.1 -0.9 -1.1 -0.1 +0.0
carbon source Ying_AminoAcid20 rep C; time point 3 +0.4 -1.2 -1.2 +0.0 -0.0
carbon source Ying_AminoAcid20 rep B; time point 3 +0.2 -1.2 -1.0 +0.2 +0.1
carbon source carbon source D-Glucose 0.25mM -2.4 +0.2 +0.1 +0.2 +0.1
phage T7_phage 0.00015625 MOI TC4 -1.2 +0.4 -0.3 -0.3 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.4 -0.3 +0.2 +0.0 +0.2
carbon source Ying_AminoAcid20 rep C; time point 2 +0.6 -0.9 -0.8 -0.0 +0.0
carbon source casaminos (C) +0.6 -0.8 -0.7 -0.1 -0.1
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 -0.3 -0.9 -0.6 +0.4
stress Chloramphenicol 0.005 mg/ml -1.6 +0.1 +0.2 +0.2 +0.1
stress LB Aerobic with Oxytetracycline 0.00000125 mM -0.9 -0.1 +0.1 +0.5 -0.3
carbon source casaminos (C) +0.6 -0.6 -0.6 -0.1 +0.2
phage Ste41 -1.7 +0.4 -0.1 +0.8 +0.3
stress LB Anaerobic with Doxycycline hyclate 0.000000390625 mM -0.8 +0.2 -0.2 +0.3 +0.4
stress LB Anaerobic with Ampicillin 0.00000195313 mM -1.1 +0.2 +0.7 +0.3 -0.1
phage Ste41 -1.4 +0.3 -0.1 +1.5 -0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +1.2 -0.1 -0.2 +0.1 -0.7
phage T7_phage 0.0015625 MOI TC2 +1.1 +0.0 -0.7 +0.5 -0.3
phage Δdbn_10 +1.5 -0.3 +0.0 -0.3 -0.2
carbon source carbon source D-Mannose 5mM +0.3 +0.5 +0.4 -0.6 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 +1.3 -0.4 +0.0 -0.2 +0.2
stress Ciprofloxacin Hydrochloride 0.0003 mM +1.4 -0.0 -0.3 +0.2 +0.1
motility outer cut, LB soft agar motility assay +1.0 -0.4 -0.2 +0.9 +0.0
stress LB Anaerobic with Trimethoprim 0.0000003125 mM +0.9 +0.1 -0.2 +0.4 +0.4
stress LB Anaerobic with Furaltadone 0.000015625 mM +1.0 -0.1 -0.1 +0.6 +0.3
control PBS_cage_1_mice _Feces_16 hr +1.2 -0.2 +0.3 -0.0 +0.6
control PBS_cage_2_mouse_2_Cecum_40 hr +1.4 +0.1 -0.1 +0.5 +0.2
phage Papa dilution 10-1 cacl2 maltose +1.5 +0.4 +0.4 +0.8 -0.3
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