Fitness for 5 genes in Escherichia coli BW25113

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500 nthyaC and hyaD overlap by 4 nucleotideshyaD and hyaE overlap by 4 nucleotideshyaE and hyaF overlap by 4 nucleotideshyaF and appC are separated by 133 nucleotides b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996 hyaC b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580 hyaD b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975 hyaE b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829 hyaF b0978: appC - cytochrome bd-II oxidase, subunit I (NCBI), at 1,036,963 to 1,038,507 appC
Group Condition b0974 b0975 b0976 b0977 b0978
hyaC hyaD hyaE hyaF appC
phage T7_phage 0.0015625 MOI TC2 -0.5 -0.3 -0.4 -0.7 -0.8
stress 3-methyl-1-butanol 1.25 vol% -1.1 -0.0 -1.0 -0.2 -0.3
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM -0.7 -0.3 -1.2 -0.1 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.2 -1.4 -0.5 +0.1
stress Aluminum chloride 5 mM -0.9 -1.2 +0.0 +0.5 -0.0
stress LB Anaerobic with Levofloxacin 0.0000000625 mM -0.5 +0.1 -0.4 -1.0 +0.3
phage T7_phage 0.00015625 MOI TC3 +0.2 +0.2 -1.0 -0.7 -0.1
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM -0.2 +0.4 -0.9 -0.5 -0.2
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -1.0 -0.6 +0.1 +0.4 -0.3
motility outer cut, LB soft agar motility assay +0.8 -1.1 -1.5 +0.4 +0.3
msc 22hr_MSC_rep1_highinoculum -0.7 -0.2 -0.8 +0.5 +0.1
stress LB Anaerobic with Ampicillin 0.00000195313 mM +0.3 -0.6 -1.1 +0.3 +0.0
stress Ciprofloxacin Hydrochloride 0.0024 mM -1.3 +0.5 -0.9 +0.8 -0.0
phage N4_phage 0.009375 MOI TC1 -1.3 +0.5 +0.2 -0.3 +0.1
phage TP4 -1.2 +0.3 +0.4 +0.3 +0.1
phage Ste41 +0.6 +0.6 -0.5 -0.9 +0.1
carbon source carbon source D-Mannose 0.5mM +0.9 +0.6 -1.0 -0.5 -0.1
phage R2_N4_T2_phage10 +0.7 -0.4 -0.6 +0.1 +0.4
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.5 -0.4 +0.9 -0.5 -0.0
phage T7_phage 0.00015625 MOI TC4 +0.6 -0.1 +0.8 -1.2 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.9 +0.2 -0.8 -0.3 +0.4
phage T7_phage 0.0015625 MOI TC1 +0.7 -0.1 +0.7 -0.6 +0.1
stress LB Anaerobic with Spiramycin 0.000125 mM +0.7 +0.4 -0.6 +0.5 -0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.5 +0.1 +0.4 +0.7 +0.5
gfp_control GFP_control +0.9 +0.2 +0.7 -0.5 +0.1
motility outer cut, LB soft agar motility assay +0.6 +0.5 +0.8 -0.1 -0.1
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +0.4 +0.5 +1.0 -0.5 +0.3
predation 6 hr rep1_HD100_10fold_dilution_grewON +1.5 +0.4 +0.4 -0.7 +0.2
mzaabcde mzaABCDE +1.3 +0.3 +0.3 -0.3 +0.3
stress nitrite 20 mM +0.3 +0.5 +1.2 +0.2 -0.3
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