Fitness for 5 genes in Escherichia coli BW25113

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500 ntyccT and b0965 are separated by 172 nucleotidesb0965 and hspQ are separated by 44 nucleotideshspQ and yccW are separated by 57 nucleotidesyccW and yccX are separated by 94 nucleotides b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965 b0966: hspQ - DNA-binding protein, hemimethylated (RefSeq), at 1,027,627 to 1,027,944 hspQ b0967: yccW - predicted methyltransferase (RefSeq), at 1,028,002 to 1,029,192 yccW b0968: yccX - predicted acylphosphatase (NCBI), at 1,029,287 to 1,029,565 yccX
Group Condition b0964 b0965 b0966 b0967 b0968
yccT b0965 hspQ yccW yccX
phage Ste41 -0.8 -1.0 -0.8 -0.5 -0.4
phage N4_phage 0.00009375 MOI TC4 -1.2 -0.6 -1.0 +0.4 -0.5
carbon source Guanosine 0.35306 mM (C) -1.0 -0.9 -0.2 +0.0 -0.8
carbon source Adenosine 0.3742 mM (C) -1.8 +0.1 -0.4 -0.4 -0.2
carbon source carbon source D-Glucose 20mM -0.3 -1.9 +0.0 -0.4 +0.1
phage R1_N4_T2_phage8 -0.4 -0.5 +0.5 -0.1 -1.6
carbon source carbon source D-Glucose 0.25mM -0.7 -1.4 -0.1 -0.1 +0.3
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 -1.1 +0.3 -0.0 -1.1
carbon source Ying_Nucleobase14 rep B; time point 3 -1.7 +0.7 -0.0 +0.1 -0.0
carbon source Acetate (C) +0.1 +0.4 -0.9 +0.3 -0.8
carbon source Acetate (C) +0.0 -0.4 -1.3 +0.5 +0.4
carbon source Uridine 0.4095 mM (C) -1.8 +0.5 +0.3 +0.1 +0.3
phage Δdbn_10 +0.2 -0.7 +0.9 -0.2 -0.8
carbon source carbon source D-Mannose 1mM -1.0 -0.4 +0.8 +0.0 +0.4
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.9 +0.5 -0.6 +0.3 +0.6
stress methylglyoxal 0.008 vol% +0.4 -0.7 -0.5 +0.5 +0.5
stress 5-Hydroxymethylfurfural 20 mM +0.9 -0.5 -0.4 +0.4 +0.2
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +1.0 -1.0 +0.7 +0.4 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +1.0 -1.1 -0.3 +1.0 +0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.0 +1.7 -0.4 -0.4 +0.6
stress Bacitracin 0.5 mg/ml +0.5 +1.2 -0.7 +0.3 +0.2
stress Bacitracin 1 mg/ml +0.5 +1.6 -0.5 +0.1 +0.0
stress Carbenicillin 0.004 mg/ml +0.8 +0.9 -0.5 +0.3 +0.2
stress Tetracycline 0.0008 mg/ml +0.5 +1.6 -0.6 +0.3 -0.0
stress Dimethyl Sulfoxide 7.5 vol% +1.1 +0.4 -0.2 +0.5 +0.5
phage R2_N4_T2_phage10 -0.2 +1.5 -0.0 +0.2 +0.7
stress acetate 140 mM +1.0 +0.7 -0.1 +0.5 +0.3
stress Cephalothin 0.01 mg/ml -0.2 +2.6 -0.2 +0.0 +0.2
motility inner cut, LB soft agar motility assay +0.2 +2.1 +0.0 +0.8 +0.3
stress Carbenicillin 0.008 mg/ml +0.2 +3.4 -0.5 +0.1 +0.3
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