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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yccF and helD are separated by 122 nucleotides
helD and mgsA are separated by 31 nucleotides
mgsA and yccT are separated by 95 nucleotides
yccT and b0965 are separated by 172 nucleotides
b0961: yccF - conserved inner membrane protein (NCBI), at 1,023,125 to 1,023,571
yccF
b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748
helD
b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238
mgsA
b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996
yccT
b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582
b0965
Group
Condition
b0961
b0962
b0963
b0964
b0965
yccF
helD
mgsA
yccT
b0965
carbon source
carbon source D-Glucose 0.25mM
+0.1
-0.4
-0.7
-0.7
-1.4
phage
N4_phage 0.00009375 MOI TC4
-0.4
-0.4
+0.1
-1.2
-0.6
phage
N4_phage 0.009375 MOI TC2
-0.4
-1.3
+0.2
-0.5
-0.5
carbon source
Adenosine 0.3742 mM (C)
+0.1
-0.4
-0.3
-1.8
+0.1
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-1.3
-0.5
+0.3
-0.4
-0.1
carbon source
D-Gluconic Acid (C)
-0.1
-0.3
-1.4
-0.4
+0.3
carbon source
carbon source D-Glucose 20mM
+0.2
+0.0
+0.2
-0.3
-1.9
control
PBS_cage_2_mouse_1_Cecum_40 hr
-1.3
+0.0
+0.3
+0.3
-1.0
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.1
-0.4
-0.2
-1.7
+0.7
phage
N4_phage 0.0009375 MOI TC3
-0.6
-0.4
-0.3
-0.9
+0.6
phage
Δdbn_10
-1.6
+0.5
+0.4
+0.2
-0.7
carbon source
D-Galacturonic Acid (C) MOPS
+0.6
-0.1
-1.7
+0.3
+0.1
phage
Ste41
+0.4
+0.6
+0.2
-0.8
-1.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.5
-0.7
-0.4
-0.1
+1.0
carbon source
D-Serine (C)
+1.0
-0.0
-0.1
-0.5
-0.9
phage
T7_phage 0.00015625 MOI TC4
+0.2
+1.0
-0.4
-0.1
-1.1
carbon source
D-Galacturonic Acid (C) M9
+0.4
+0.2
-1.4
+0.7
+0.4
stress
Benzalkonium Chloride 0.004 mg/ml
+0.5
-0.0
+0.1
+1.0
-1.1
phage
T7_phage 0.0015625 MOI TC2
-0.6
+0.7
-0.1
-0.2
+1.0
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.7
+0.5
-0.1
-0.9
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.9
+0.4
+0.2
+0.3
+0.9
stress
LB Aerobic with Tetracycline 0.00000125 mM
+0.5
-0.1
-0.4
+0.6
+0.8
stress
Tetracycline 0.0008 mg/ml
-0.2
-0.1
-0.2
+0.5
+1.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.5
+0.4
+0.4
+0.6
+1.0
stress
Cephalothin 0.01 mg/ml
-0.2
-0.2
+0.0
-0.2
+2.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
+1.1
-0.5
+0.2
+0.1
+1.3
stress
Bacitracin 1 mg/ml
-0.5
+0.7
-0.2
+0.5
+1.6
motility
inner cut, LB soft agar motility assay
-0.2
+0.2
+0.1
+0.2
+2.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+2.0
+0.0
+0.2
+0.6
+0.0
stress
Carbenicillin 0.008 mg/ml
-0.0
-0.1
-0.1
+0.2
+3.4
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