Fitness for 5 genes in Escherichia coli BW25113

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500 ntyccF and helD are separated by 122 nucleotideshelD and mgsA are separated by 31 nucleotidesmgsA and yccT are separated by 95 nucleotidesyccT and b0965 are separated by 172 nucleotides b0961: yccF - conserved inner membrane protein (NCBI), at 1,023,125 to 1,023,571 yccF b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748 helD b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238 mgsA b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965
Group Condition b0961 b0962 b0963 b0964 b0965
yccF helD mgsA yccT b0965
carbon source carbon source D-Glucose 0.25mM +0.1 -0.4 -0.7 -0.7 -1.4
phage N4_phage 0.00009375 MOI TC4 -0.4 -0.4 +0.1 -1.2 -0.6
phage N4_phage 0.009375 MOI TC2 -0.4 -1.3 +0.2 -0.5 -0.5
carbon source Adenosine 0.3742 mM (C) +0.1 -0.4 -0.3 -1.8 +0.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -1.3 -0.5 +0.3 -0.4 -0.1
carbon source D-Gluconic Acid (C) -0.1 -0.3 -1.4 -0.4 +0.3
carbon source carbon source D-Glucose 20mM +0.2 +0.0 +0.2 -0.3 -1.9
control PBS_cage_2_mouse_1_Cecum_40 hr -1.3 +0.0 +0.3 +0.3 -1.0
carbon source Ying_Nucleobase14 rep B; time point 3 -0.1 -0.4 -0.2 -1.7 +0.7
phage N4_phage 0.0009375 MOI TC3 -0.6 -0.4 -0.3 -0.9 +0.6
phage Δdbn_10 -1.6 +0.5 +0.4 +0.2 -0.7
carbon source D-Galacturonic Acid (C) MOPS +0.6 -0.1 -1.7 +0.3 +0.1
phage Ste41 +0.4 +0.6 +0.2 -0.8 -1.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.5 -0.7 -0.4 -0.1 +1.0
carbon source D-Serine (C) +1.0 -0.0 -0.1 -0.5 -0.9
phage T7_phage 0.00015625 MOI TC4 +0.2 +1.0 -0.4 -0.1 -1.1
carbon source D-Galacturonic Acid (C) M9 +0.4 +0.2 -1.4 +0.7 +0.4
stress Benzalkonium Chloride 0.004 mg/ml +0.5 -0.0 +0.1 +1.0 -1.1
phage T7_phage 0.0015625 MOI TC2 -0.6 +0.7 -0.1 -0.2 +1.0
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.7 +0.5 -0.1 -0.9 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 +0.4 +0.2 +0.3 +0.9
stress LB Aerobic with Tetracycline 0.00000125 mM +0.5 -0.1 -0.4 +0.6 +0.8
stress Tetracycline 0.0008 mg/ml -0.2 -0.1 -0.2 +0.5 +1.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 +0.4 +0.4 +0.6 +1.0
stress Cephalothin 0.01 mg/ml -0.2 -0.2 +0.0 -0.2 +2.6
stress LB Aerobic with Ramoplanin 0.000125 mM +1.1 -0.5 +0.2 +0.1 +1.3
stress Bacitracin 1 mg/ml -0.5 +0.7 -0.2 +0.5 +1.6
motility inner cut, LB soft agar motility assay -0.2 +0.2 +0.1 +0.2 +2.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +2.0 +0.0 +0.2 +0.6 +0.0
stress Carbenicillin 0.008 mg/ml -0.0 -0.1 -0.1 +0.2 +3.4
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