Fitness for 5 genes in Escherichia coli BW25113

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500 ntrmf and fabA are separated by 69 nucleotidesfabA and ycbZ are separated by 68 nucleotidesycbZ and ycbG are separated by 185 nucleotidesycbG and ompA are separated by 75 nucleotides b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf b0954: fabA - 3-hydroxydecanoyl-ACP dehydratase (NCBI), at 1,015,175 to 1,015,693 fabA b0955: ycbZ - predicted peptidase (NCBI), at 1,015,762 to 1,017,522 ycbZ b0956: ycbG - hypothetical protein (NCBI), at 1,017,708 to 1,018,160 ycbG b0957: ompA - outer membrane protein A (3a;II*;G;d) (NCBI), at 1,018,236 to 1,019,276 ompA
Group Condition b0953 b0954 b0955 b0956 b0957
rmf fabA ycbZ ycbG ompA
carbon source Ying_sugar7 rep A; time point 3 -1.1 N.D. +0.2 N.D. -7.5
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.6 N.D. +0.0 N.D. -7.8
carbon source Ying_OrganicAcid7 rep A; time point 3 -1.9 N.D. +1.2 N.D. -7.5
carbon source Ying_sugar7 rep C; time point 3 -0.7 N.D. +0.0 N.D. -7.5
carbon source Ying_OrganicAcid7 rep B; time point 3 -1.5 N.D. +1.2 N.D. -7.6
carbon source Ying_sugar7 rep B; time point 3 -0.3 N.D. +0.2 N.D. -7.6
carbon source Ying_OrganicAcid7 rep C; time point 3 -1.4 N.D. +1.2 N.D. -7.5
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.2 N.D. -0.4 N.D. -6.8
carbon source Ying_sugar7 rep C; time point 2 -0.9 N.D. +0.4 N.D. -6.2
carbon source Dodecanol and Rhamnolipid (C) -0.4 N.D. +0.1 N.D. -6.3
carbon source Ying_OrganicAcid7 rep B; time point 2 -0.9 N.D. +0.9 N.D. -6.2
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.2 N.D. +0.7 N.D. -6.5
carbon source Ying_sugar7 rep A; time point 2 -0.6 N.D. +0.4 N.D. -5.7
micoeukaryotes Dictyostellum sp.mixed culture-2 -1.0 N.D. -0.0 N.D. -4.8
stress octanol 0.08 vol% -0.8 N.D. +0.9 N.D. -6.0
micoeukaryotes Dictyostellum sp.mixed culture-1 -0.6 N.D. -0.2 N.D. -5.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -3.2 N.D. +0.9 N.D. -3.4
carbon source carbon source D-Glucose 0.25mM +0.7 N.D. +0.3 N.D. -6.5
mixed community Mixed culture; Bdellovibrio bacteriovorus HD100 1:10 starting OD to Ecoli; 0.1X M9; outgrowth in LBwithKan +0.3 N.D. -0.1 N.D. -5.8
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -0.0 N.D. +0.7 N.D. -5.7
carbon source Dodecanol and Rhamnolipid (C) +0.1 N.D. +0.5 N.D. -5.5
stress M9 with Methotrexate 1.98 mM 24 hr -1.0 N.D. +0.7 N.D. -4.2
carbon source Ying_OrganicAcid7 rep A; time point 2 -0.8 N.D. +1.2 N.D. -4.4
carbon source Ying_OrganicAcid7 rep C; time point 2 -1.0 N.D. +1.0 N.D. -3.9
stress LB Aerobic with Spiramycin 0.000125 mM +0.0 N.D. +2.5 N.D. -4.5
stress LB Aerobic with Spiramycin 0.000125 mM -0.1 N.D. +2.7 N.D. -3.6
stress LB Anaerobic with Spiramycin 0.000125 mM +0.2 N.D. +3.1 N.D. -3.8
stress Benzalkonium Chloride 0.004 mg/ml -0.0 N.D. +3.6 N.D. -4.0
stress LB Anaerobic with Spiramycin 0.000125 mM +0.2 N.D. +3.0 N.D. -3.2
stress LB Aerobic with Irgasan 0.0000003125 mM +0.7 N.D. -1.3 N.D. +4.0
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