Fitness for 5 genes in Escherichia coli BW25113

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500 ntpqiA and pqiB are separated by 4 nucleotidespqiB and ymbA overlap by 4 nucleotidesymbA and rmf are separated by 255 nucleotidesrmf and fabA are separated by 69 nucleotides b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477 pqiA b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122 pqiB b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682 ymbA b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf b0954: fabA - 3-hydroxydecanoyl-ACP dehydratase (NCBI), at 1,015,175 to 1,015,693 fabA
Group Condition b0950 b0951 b0952 b0953 b0954
pqiA pqiB ymbA rmf fabA
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -3.3 -3.5 -4.3 -3.2 N.D.
stress Benzalkonium Chloride 0.004 mg/ml -2.7 -1.7 -2.5 -0.0 N.D.
stress octanol 0.08 vol% -2.7 -1.3 -2.1 -0.8 N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -2.0 -2.6 -2.0 +0.0 N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -1.6 -2.0 -1.2 -0.7 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -1.1 -1.2 -2.1 -0.9 N.D.
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM -1.1 -1.5 -2.0 +0.1 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -1.1 -1.1 -1.1 -1.0 N.D.
stress decyl alcohol 5 vol% -1.3 -1.0 -1.5 +0.0 N.D.
no stress control M9 no stress control; Aerobic 48 hr -1.2 -1.0 -1.1 +0.2 N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.9 -1.0 -1.1 -0.1 N.D.
stress LB Anaerobic with Doxycycline hyclate 0.00000078125 mM -0.6 -0.6 -0.7 -1.0 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.7 -0.5 -1.0 -0.4 N.D.
stress decyl alcohol 5 vol% -0.9 -0.8 -0.9 +0.1 N.D.
carbon source acetate (C) +0.1 +0.3 -0.6 -2.3 N.D.
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM -0.8 -1.0 -0.8 +0.1 N.D.
mzaabcde mzaABCDE -0.6 -0.1 -0.6 -1.1 N.D.
stress LB Anaerobic with Nalidixic 0.0000078125 mM -0.8 -0.4 -0.4 -0.8 N.D.
stress LB Anaerobic with Doxycycline hyclate 0.00000078125 mM -0.9 -0.9 -0.5 +0.2 N.D.
no stress control M9 no stress control; Aerobic 24 hr -0.8 -0.7 -0.7 +0.3 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.6 -0.2 +0.5 -1.2 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.7 -0.1 +0.2 -2.0 N.D.
stress Aluminum chloride 5 mM +0.4 +0.5 +0.3 -1.5 N.D.
phage Ste41 -0.5 +0.7 +1.0 -1.0 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.6 -0.2 -0.4 +1.7 N.D.
phage RB68 +0.3 +0.4 +1.2 -1.2 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.6 +0.6 +0.6 -0.8 N.D.
motility inner cut, LB soft agar motility assay +0.6 +0.6 -0.5 +1.0 N.D.
carbon source D-Ribose (C) -0.1 +0.1 -0.2 +2.4 N.D.
carbon source D-Ribose (C) -0.0 +0.0 +0.2 +2.3 N.D.
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