Fitness for 5 genes in Escherichia coli BW25113

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500 ntuup and pqiA are separated by 129 nucleotidespqiA and pqiB are separated by 4 nucleotidespqiB and ymbA overlap by 4 nucleotidesymbA and rmf are separated by 255 nucleotides b0949: uup - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,009,187 to 1,011,094 uup b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477 pqiA b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122 pqiB b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682 ymbA b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf
Group Condition b0949 b0950 b0951 b0952 b0953
uup pqiA pqiB ymbA rmf
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -3.6 -3.3 -3.5 -4.3 -3.2
stress Benzalkonium Chloride 0.004 mg/ml -1.6 -2.7 -1.7 -2.5 -0.0
stress octanol 0.08 vol% -0.9 -2.7 -1.3 -2.1 -0.8
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -0.3 -2.0 -2.6 -2.0 +0.0
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM -2.2 -1.1 -1.5 -2.0 +0.1
lb LB regrowth after 48hrs -6.0 +0.1 -0.0 +0.0 -0.6
stress decyl alcohol 5 vol% -2.3 -1.3 -1.0 -1.5 +0.0
lb LB regrowth after 48hrs -5.7 -0.1 +0.0 +0.1 -0.1
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -5.4 +0.1 +0.1 +0.2 -0.6
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -0.2 -1.6 -2.0 -1.2 -0.7
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 -1.1 -1.2 -2.1 -0.9
stress LB + ciprofloxacin regrowth after 48hrs -5.3 +0.0 +0.1 +0.2 -0.2
stress decyl alcohol 5 vol% -2.4 -0.9 -0.8 -0.9 +0.1
motility outer cut, LB soft agar motility assay -4.6 -0.3 +0.2 +0.6 -0.8
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM -2.3 -0.8 -1.0 -0.8 +0.1
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -3.4 +0.7 -0.1 +0.2 -2.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 -1.1 -1.1 -1.1 -1.0
stress Cisplatin 0.1 mg/ml -3.3 -0.4 -0.2 -0.4 -0.2
stress Bacitracin 1 mg/ml -3.9 -0.1 +0.4 +0.1 -1.0
control PBS_cage_1_mice _Feces_16 hr -2.5 -0.3 +0.1 +0.2 -1.5
stress Cisplatin 0.1 mg/ml -3.6 -0.0 -0.1 -0.5 +0.4
stress Bacitracin 0.5 mg/ml -3.5 -0.2 +0.2 +0.4 -0.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -4.3 +0.6 +0.6 +0.6 -0.8
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -4.1 +0.4 +0.6 +0.2 -0.3
control PBS_cage_1_mouse_2_Cecum_40 hr -3.4 -0.1 -0.4 +0.5 +0.3
stress Butyrolactam 3 vol% -3.9 +0.1 +0.2 +0.2 +0.3
stress Aluminum chloride 5 mM -2.4 +0.4 +0.5 +0.3 -1.5
control PBS_cage_1_mouse_1_Cecum_40 hr -2.9 +0.1 -0.6 -0.1 +1.6
motility inner cut, LB soft agar motility assay -2.9 +0.6 +0.6 -0.5 +1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +2.8 -0.2 -0.1 +0.5 +1.1
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