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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ycbY and uup are separated by 11 nucleotides
uup and pqiA are separated by 129 nucleotides
pqiA and pqiB are separated by 4 nucleotides
pqiB and ymbA overlap by 4 nucleotides
b0948: ycbY - predicted methyltransferase (NCBI), at 1,007,067 to 1,009,175
ycbY
b0949: uup - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,009,187 to 1,011,094
uup
b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477
pqiA
b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122
pqiB
b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682
ymbA
Group
Condition
b0948
b0949
b0950
b0951
b0952
ycbY
uup
pqiA
pqiB
ymbA
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.5
-3.6
-3.3
-3.5
-4.3
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
-1.6
-2.7
-1.7
-2.5
stress
octanol 0.08 vol%
-0.6
-0.9
-2.7
-1.3
-2.1
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
-0.4
-2.2
-1.1
-1.5
-2.0
stress
LB Aerobic with Benzethonium chloride 0.000015625 mM
-0.3
-0.3
-2.0
-2.6
-2.0
lb
LB regrowth after 48hrs
-1.1
-6.0
+0.1
-0.0
+0.0
lb
LB regrowth after 48hrs
-1.2
-5.7
-0.1
+0.0
+0.1
stress
decyl alcohol 5 vol%
-0.4
-2.3
-1.3
-1.0
-1.5
motility
outer cut, LB soft agar motility assay
-2.3
-4.6
-0.3
+0.2
+0.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-1.1
-5.4
+0.1
+0.1
+0.2
stress
LB + ciprofloxacin regrowth after 48hrs
-1.0
-5.3
+0.0
+0.1
+0.2
stress
Cisplatin 0.1 mg/ml
-1.5
-3.6
-0.0
-0.1
-0.5
stress
Cisplatin 0.1 mg/ml
-1.3
-3.3
-0.4
-0.2
-0.4
stress
decyl alcohol 5 vol%
-0.5
-2.4
-0.9
-0.8
-0.9
stress
Cisplatin 0.1 mg/ml
-1.1
-3.5
-0.1
-0.3
-0.2
stress
LB Aerobic with Benzethonium chloride 0.000015625 mM
-0.1
-0.2
-1.6
-2.0
-1.2
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
-0.2
-2.3
-0.8
-1.0
-0.8
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.2
-0.3
-1.1
-1.2
-2.1
stress
Cisplatin 0.05 mg/ml
-1.3
-3.6
+0.1
-0.2
+0.2
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-0.9
-3.0
-0.4
-0.4
-0.1
gfp_control
GFP_control
-0.8
-2.3
-0.9
+0.1
-0.5
stress
Butyrolactam 3 vol%
-0.9
-3.9
+0.1
+0.2
+0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.8
-3.4
-0.1
-0.4
+0.5
stress
Cisplatin 0.1 mg/ml
-1.6
-2.6
+0.4
+0.0
-0.3
stress
Bacitracin 1 mg/ml
-0.4
-3.9
-0.1
+0.4
+0.1
stress
Bacitracin 0.5 mg/ml
-0.6
-3.5
-0.2
+0.2
+0.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.6
-4.1
+0.4
+0.6
+0.2
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.9
-3.4
+0.7
-0.1
+0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.6
-4.3
+0.6
+0.6
+0.6
motility
inner cut, LB soft agar motility assay
-0.2
-2.9
+0.6
+0.6
-0.5
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