Fitness for 5 genes in Escherichia coli BW25113

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500 ntasnS and pncB are separated by 168 nucleotidespncB and pepN are separated by 265 nucleotidespepN and ssuB are separated by 42 nucleotidesssuB and ycbM overlap by 4 nucleotides b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208 asnS b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579 pncB b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457 pepN b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267 ssuB b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055 ycbM
Group Condition b0930 b0931 b0932 b0933 b0934
asnS pncB pepN ssuB ycbM
phage Ste41 N.D. -0.2 -0.2 -1.0 -4.6
phage Ste41 N.D. -0.6 -0.0 -0.5 -4.7
phage T7_phage 0.00015625 MOI TC4 N.D. -2.0 -0.8 -1.0 -1.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. -1.8 +0.6 -0.5 -2.4
carbon source carbon source D-Mannose 5mM N.D. -0.2 -1.0 +0.1 -1.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -1.5 -0.1 +0.7 -1.6
carbon source Guanosine 0.35306 mM (C) N.D. -0.6 +0.1 -0.1 -1.8
phage N4_phage 0.009375 MOI TC2 N.D. +0.5 -0.2 -0.2 -2.5
stress 2,4-Dinitrophenol 2 mM N.D. -0.9 -0.8 +0.1 -0.6
nophagecontrol R1_N4_T1_phage0 N.D. -0.2 -0.3 +0.1 -1.8
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM N.D. -1.3 +0.1 -0.0 -0.8
stress Cisplatin 0.1 mg/ml N.D. +0.5 -1.0 -0.5 -1.0
carbon source carbon source D-Mannose 0.5mM N.D. -0.2 -0.3 +0.5 -1.4
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.2 -0.8 -0.0 -1.6
stress Cisplatin 0.1 mg/ml N.D. -0.7 -1.0 -0.2 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.6 -0.6 -0.9 -1.3
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.5 -1.7 -0.1 -0.9
carbon source Glycolic Acid (C) N.D. +0.7 -1.5 -0.2 +0.0
stress Trimethoprim 0.0025 mM N.D. +0.8 -0.9 +0.5 -1.0
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +1.2 -0.3 -0.2 -0.9
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -1.2 +0.4 +0.6 -0.1
stress LB Aerobic with Trimethoprim 0.00000125 mM N.D. +1.0 -0.4 -0.4 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +1.2 -0.6 -0.0 -0.6
carbon source Glycolic Acid (C) N.D. +1.4 -1.4 +0.3 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.3 -1.1 +0.0 -0.1
stress Aluminum chloride 5 mM N.D. +1.7 -1.2 -0.5 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.6 -0.8 +0.0 -0.4
stress LB Anaerobic with Trimethoprim 0.0000003125 mM N.D. +1.7 +0.0 -0.2 -0.7
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. +2.1 -0.3 +0.2 -0.4
stress Trimethoprim 0.005 mM N.D. +1.8 -0.1 -0.2 +2.2
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