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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ompF and asnS are separated by 602 nucleotides
asnS and pncB are separated by 168 nucleotides
pncB and pepN are separated by 265 nucleotides
pepN and ssuB are separated by 42 nucleotides
b0929: ompF - outer membrane porin 1a (Ia;b;F) (NCBI), at 985,117 to 986,205
ompF
b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208
asnS
b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579
pncB
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
Group
Condition
b0929
b0930
b0931
b0932
b0933
ompF
asnS
pncB
pepN
ssuB
phage
T7_phage 0.00015625 MOI TC4
-1.1
N.D.
-2.0
-0.8
-1.0
phage
phage
-3.1
N.D.
-0.1
-0.2
-0.2
phage
T7_phage 0.0015625 MOI TC1
-2.4
N.D.
-0.4
-0.1
-0.7
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.4
N.D.
-1.8
+0.6
-0.5
carbon source
Glycolic Acid (C)
-1.2
N.D.
+0.7
-1.5
-0.2
carbon source
Glycolic Acid (C)
-1.2
N.D.
+1.4
-1.4
+0.3
stress
Aluminum chloride 5 mM
-0.4
N.D.
+1.7
-1.2
-0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.7
N.D.
+1.5
-1.7
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.4
N.D.
+1.6
-0.6
-0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.3
N.D.
+1.6
-0.8
+0.0
stress
LB Anaerobic with Norfloxacin 0.00000015625 mM
+3.0
N.D.
-0.5
+0.1
-0.2
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+3.7
N.D.
-1.3
+0.1
-0.0
predation
6 hr rep1_HD100_10fold_dilution_grewON
+3.3
N.D.
+0.1
-0.2
-0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
+2.8
N.D.
+0.0
-0.3
+0.5
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+2.5
N.D.
+1.2
-0.3
-0.2
stress
Tetracycline 0.0008 mg/ml
+3.8
N.D.
-0.4
-0.0
-0.1
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM
+3.6
N.D.
-0.1
+0.2
-0.3
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+4.1
N.D.
-1.0
+0.2
+0.2
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
+4.1
N.D.
-0.2
-0.1
-0.2
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
+3.2
N.D.
-0.0
+0.2
+0.3
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM
+3.1
N.D.
-0.1
+0.3
+0.4
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+4.7
N.D.
-0.1
+0.0
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+5.3
N.D.
-1.2
+0.4
+0.6
stress
Carbenicillin 0.008 mg/ml
+5.8
N.D.
+0.1
+0.2
-0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+6.0
N.D.
-0.1
+0.1
+0.1
stress
LB Anaerobic with Norfloxacin 0.00000015625 mM
+6.9
N.D.
-0.5
-0.0
-0.3
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+6.7
N.D.
-0.1
+0.1
-0.0
stress
Cephalothin 0.01 mg/ml
+6.1
N.D.
-0.1
+0.4
+0.4
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+7.8
N.D.
+0.3
+0.1
-0.8
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+8.4
N.D.
-0.3
+0.1
+0.3
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