Fitness for 5 genes in Escherichia coli BW25113

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500 ntompF and asnS are separated by 602 nucleotidesasnS and pncB are separated by 168 nucleotidespncB and pepN are separated by 265 nucleotidespepN and ssuB are separated by 42 nucleotides b0929: ompF - outer membrane porin 1a (Ia;b;F) (NCBI), at 985,117 to 986,205 ompF b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208 asnS b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579 pncB b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457 pepN b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267 ssuB
Group Condition b0929 b0930 b0931 b0932 b0933
ompF asnS pncB pepN ssuB
phage T7_phage 0.00015625 MOI TC4 -1.1 N.D. -2.0 -0.8 -1.0
phage phage -3.1 N.D. -0.1 -0.2 -0.2
phage T7_phage 0.0015625 MOI TC1 -2.4 N.D. -0.4 -0.1 -0.7
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.4 N.D. -1.8 +0.6 -0.5
carbon source Glycolic Acid (C) -1.2 N.D. +0.7 -1.5 -0.2
carbon source Glycolic Acid (C) -1.2 N.D. +1.4 -1.4 +0.3
stress Aluminum chloride 5 mM -0.4 N.D. +1.7 -1.2 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 N.D. +1.5 -1.7 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.4 N.D. +1.6 -0.6 -0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.3 N.D. +1.6 -0.8 +0.0
stress LB Anaerobic with Norfloxacin 0.00000015625 mM +3.0 N.D. -0.5 +0.1 -0.2
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +3.7 N.D. -1.3 +0.1 -0.0
predation 6 hr rep1_HD100_10fold_dilution_grewON +3.3 N.D. +0.1 -0.2 -0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON +2.8 N.D. +0.0 -0.3 +0.5
stress LB Anaerobic with Furaltadone 0.000015625 mM +2.5 N.D. +1.2 -0.3 -0.2
stress Tetracycline 0.0008 mg/ml +3.8 N.D. -0.4 -0.0 -0.1
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM +3.6 N.D. -0.1 +0.2 -0.3
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +4.1 N.D. -1.0 +0.2 +0.2
stress LB Anaerobic with Ampicillin 0.00000195313 mM +4.1 N.D. -0.2 -0.1 -0.2
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM +3.2 N.D. -0.0 +0.2 +0.3
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM +3.1 N.D. -0.1 +0.3 +0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM +4.7 N.D. -0.1 +0.0 +0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +5.3 N.D. -1.2 +0.4 +0.6
stress Carbenicillin 0.008 mg/ml +5.8 N.D. +0.1 +0.2 -0.2
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +6.0 N.D. -0.1 +0.1 +0.1
stress LB Anaerobic with Norfloxacin 0.00000015625 mM +6.9 N.D. -0.5 -0.0 -0.3
stress LB Anaerobic with Cefoxitin 0.000003125 mM +6.7 N.D. -0.1 +0.1 -0.0
stress Cephalothin 0.01 mg/ml +6.1 N.D. -0.1 +0.4 +0.4
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +7.8 N.D. +0.3 +0.1 -0.8
stress LB Anaerobic with Cefoxitin 0.000003125 mM +8.4 N.D. -0.3 +0.1 +0.3
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