Fitness for 5 genes in Escherichia coli BW25113

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500 ntaqpZ and ybjD are separated by 425 nucleotidesybjD and ybjX overlap by 4 nucleotidesybjX and b0878 are separated by 114 nucleotidesb0878 and macB overlap by 4 nucleotides b0875: aqpZ - aquaporin Z (NCBI), at 914,575 to 915,270 aqpZ b0876: ybjD - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 915,696 to 917,354 ybjD b0877: ybjX - hypothetical protein (NCBI), at 917,351 to 918,343 ybjX b0878: b0878 - putative membrane protein (VIMSS), at 918,458 to 919,573 b0878 b0879: macB - fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component (NCBI), at 919,570 to 921,516 macB
Group Condition b0875 b0876 b0877 b0878 b0879
aqpZ ybjD ybjX b0878 macB
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.9 -0.2 -3.8 +0.1 +0.1
stress Benzalkonium Chloride 0.004 mg/ml -0.3 -0.3 -3.3 +0.8 +0.6
carbon source carbon source D-Glucose 0.25mM -0.3 -0.2 -0.6 -0.9 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.4 -0.4 -1.8 -0.2 -0.1
phage TP4 +0.0 -0.6 -0.5 -0.8 +0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -0.1 -3.6 +1.0 +1.1
phage R2_N4_T1_phage10 -0.4 +0.3 -0.7 -1.0 +0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.1 -0.3 -1.0 +0.0 -0.4
stress Nalidixic 0.006 mg/ml +0.0 -0.1 -1.7 +0.1 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -0.4 -0.9 +0.1 +0.1
stress octanol 0.08 vol% -0.5 -0.2 -1.1 +0.4 -0.2
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM -0.1 -0.0 -1.7 +0.2 +0.1
stress LB Anaerobic with Doxycycline hyclate 0.00000078125 mM +0.2 +0.1 -1.3 -0.2 -0.2
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM -0.0 -0.1 -1.4 +0.3 -0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 +0.2 -1.4 -0.1 +0.0
stress LB Aerobic with Doxycycline hyclate 0.00000625 mM +0.5 -0.1 -1.0 -0.3 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 -0.0 -3.6 +1.2 +1.3
phage Ste41 +0.3 +0.7 +0.3 -1.7 -0.6
stress Cephalothin 0.01 mg/ml +0.4 +0.2 -1.7 +0.4 +0.1
stress Cisplatin 0.1 mg/ml -0.0 +0.1 +0.5 -0.6 -0.6
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +0.3 -0.1 -1.1 +0.2 +0.1
stress Doxycycline hyclate 0.001 mg/ml +0.3 +0.3 -2.0 +0.8 -0.0
stress Bacitracin 0.5 mg/ml -0.4 +0.1 +1.2 -0.4 -0.7
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.1 -0.1 +0.9 -0.5 -0.4
stress LB Anaerobic with Ampicillin 0.00000195313 mM -0.2 +0.2 -0.4 +0.9 -0.2
control PBS_cage_2_mouse_1_Cecum_40 hr +0.3 +0.3 -0.7 +0.4 +0.2
phage T7_phage 0.00015625 MOI TC4 +0.4 +0.9 +0.4 -0.3 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.0 +0.3 -0.4 +0.1 +0.2
stress Bacitracin 1 mg/ml +0.1 +0.4 +4.3 -1.2 -2.2
stress 3-methyl-1-butanol 1.25 vol% +0.6 -0.2 +0.3 +0.1 +0.7
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