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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ybjR and b0868 overlap by 4 nucleotides
b0868 and ybjT are separated by 98 nucleotides
ybjT and ltaE are separated by 10 nucleotides
ltaE and poxB are separated by 36 nucleotides
b0867: ybjR - predicted amidase and lipoprotein (NCBI), at 904,136 to 904,966
ybjR
b0868: b0868 - putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS), at 904,963 to 905,976
b0868
b0869: ybjT - putative dTDP-glucose enzyme (VIMSS), at 906,075 to 907,505
ybjT
b0870: ltaE - L-allo-threonine aldolase, PLP-dependent (NCBI), at 907,516 to 908,517
ltaE
b0871: poxB - pyruvate dehydrogenase (NCBI), at 908,554 to 910,272
poxB
Group
Condition
b0867
b0868
b0869
b0870
b0871
ybjR
b0868
ybjT
ltaE
poxB
stress
2,4-Dinitrophenol 2 mM
-0.0
+0.0
-0.3
-0.7
-4.4
stress
2,4-Dinitrophenol 2 mM
+0.2
-0.0
-0.4
-0.2
-3.5
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.9
-2.0
+0.4
-0.1
-0.2
phage
T7_phage 0.0015625 MOI TC2
-0.6
-0.9
-0.5
+0.1
-0.2
stress
Bacitracin 1 mg/ml
-0.6
-1.2
+0.2
+0.2
-0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.1
-0.5
+0.2
-0.5
+0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
-2.1
+0.3
+0.6
+0.2
stress
LB Anaerobic with Spiramycin 0.000125 mM
-0.2
-1.5
+0.4
-0.1
+0.1
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.0
-1.0
+0.6
-0.1
+0.2
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.4
-0.6
-1.1
+0.3
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.3
-1.4
+0.7
+0.2
-0.2
phage
Lambda_ci1857
-0.4
+0.4
-0.4
-0.6
+0.2
phage
T7_phage 0.00015625 MOI TC4
-0.4
-0.2
+0.6
-0.3
-0.3
stress
Chloramphenicol 0.0025 mg/ml
-0.0
-0.4
-0.7
+0.4
+0.2
stress
LB Anaerobic with Spiramycin 0.000125 mM
-0.2
-1.3
+0.5
+0.4
+0.1
stress
Chlorite 1.25 mM
-0.5
-0.2
-0.6
+0.7
+0.3
stress
Spectinomycin 0.0125 mg/ml
-0.1
-0.9
+0.3
+0.6
-0.2
stress
1-ethyl-3-methylimidazolium chloride 40 mM
-0.2
+0.1
-0.3
+0.8
-0.3
stress
Aluminum chloride 5 mM
+0.2
-0.1
-0.8
+1.1
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-0.4
+1.0
+0.3
-0.2
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
-0.1
-0.5
+0.5
+0.5
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.5
-0.3
+1.1
+0.3
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
-0.5
+1.5
+0.4
-0.2
stress
Cisplatin 0.1 mg/ml
+0.7
-0.7
+0.2
+0.7
+0.5
plasmid transformation
vcDART vector transformation; agar assay
-0.2
-0.0
+1.3
+0.6
-0.2
plasmid transformation
vcDART vector transformation; agar assay
+0.1
+0.0
+1.1
+0.4
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.0
-0.1
+0.8
+0.8
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.5
+0.3
+0.3
+0.3
stress
Benzalkonium Chloride 0.004 mg/ml
-0.2
-0.3
+1.0
+1.5
-0.2
plasmid transformation
vcDART vector transformation; agar assay
+0.1
+0.1
+1.1
+0.7
+0.0
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