Fitness for 5 genes in Escherichia coli BW25113

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500 ntybjR and b0868 overlap by 4 nucleotidesb0868 and ybjT are separated by 98 nucleotidesybjT and ltaE are separated by 10 nucleotidesltaE and poxB are separated by 36 nucleotides b0867: ybjR - predicted amidase and lipoprotein (NCBI), at 904,136 to 904,966 ybjR b0868: b0868 - putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS), at 904,963 to 905,976 b0868 b0869: ybjT - putative dTDP-glucose enzyme (VIMSS), at 906,075 to 907,505 ybjT b0870: ltaE - L-allo-threonine aldolase, PLP-dependent (NCBI), at 907,516 to 908,517 ltaE b0871: poxB - pyruvate dehydrogenase (NCBI), at 908,554 to 910,272 poxB
Group Condition b0867 b0868 b0869 b0870 b0871
ybjR b0868 ybjT ltaE poxB
stress 2,4-Dinitrophenol 2 mM -0.0 +0.0 -0.3 -0.7 -4.4
stress 2,4-Dinitrophenol 2 mM +0.2 -0.0 -0.4 -0.2 -3.5
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.9 -2.0 +0.4 -0.1 -0.2
phage T7_phage 0.0015625 MOI TC2 -0.6 -0.9 -0.5 +0.1 -0.2
stress Bacitracin 1 mg/ml -0.6 -1.2 +0.2 +0.2 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.1 -0.5 +0.2 -0.5 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.9 -2.1 +0.3 +0.6 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM -0.2 -1.5 +0.4 -0.1 +0.1
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.0 -1.0 +0.6 -0.1 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 -0.6 -1.1 +0.3 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.3 -1.4 +0.7 +0.2 -0.2
phage Lambda_ci1857 -0.4 +0.4 -0.4 -0.6 +0.2
phage T7_phage 0.00015625 MOI TC4 -0.4 -0.2 +0.6 -0.3 -0.3
stress Chloramphenicol 0.0025 mg/ml -0.0 -0.4 -0.7 +0.4 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM -0.2 -1.3 +0.5 +0.4 +0.1
stress Chlorite 1.25 mM -0.5 -0.2 -0.6 +0.7 +0.3
stress Spectinomycin 0.0125 mg/ml -0.1 -0.9 +0.3 +0.6 -0.2
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.2 +0.1 -0.3 +0.8 -0.3
stress Aluminum chloride 5 mM +0.2 -0.1 -0.8 +1.1 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.4 +1.0 +0.3 -0.2
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM -0.1 -0.5 +0.5 +0.5 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -0.3 +1.1 +0.3 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 -0.5 +1.5 +0.4 -0.2
stress Cisplatin 0.1 mg/ml +0.7 -0.7 +0.2 +0.7 +0.5
plasmid transformation vcDART vector transformation; agar assay -0.2 -0.0 +1.3 +0.6 -0.2
plasmid transformation vcDART vector transformation; agar assay +0.1 +0.0 +1.1 +0.4 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 -0.1 +0.8 +0.8 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.5 +0.3 +0.3 +0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.2 -0.3 +1.0 +1.5 -0.2
plasmid transformation vcDART vector transformation; agar assay +0.1 +0.1 +1.1 +0.7 +0.0
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