Fitness for 5 genes in Escherichia coli BW25113

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500 ntrybB and ybjL are separated by 79 nucleotidesybjL and ybjM are separated by 269 nucleotidesybjM and grxA are separated by 29 nucleotidesgrxA and ybjC are separated by 159 nucleotides b4417: rybB - unknown RNA (NCBI), at 887,199 to 887,277 rybB b0847: ybjL - hypothetical protein (NCBI), at 887,357 to 889,042 ybjL b0848: ybjM - predicted inner membrane protein (NCBI), at 889,312 to 889,689 ybjM b0849: grxA - glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI), at 889,719 to 889,976 grxA b0850: ybjC - predicted inner membrane protein (NCBI), at 890,136 to 890,423 ybjC
Group Condition b4417 b0847 b0848 b0849 b0850
rybB ybjL ybjM grxA ybjC
phage T7_phage 0.0015625 MOI TC2 N.D. +0.0 -0.3 -1.8 -2.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. -2.7 -0.4 -1.8 +0.7
stress Cisplatin 0.1 mg/ml N.D. -0.6 -0.4 -3.1 +0.1
phage Ste41 N.D. +0.1 -0.2 -1.4 -2.4
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -3.0 +0.0 -0.4 -0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. -2.3 -0.6 -0.9 +0.4
carbon source carbon source D-Glucose 0.25mM N.D. +0.1 -1.1 -1.4 -0.9
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. +0.4 -0.3 -2.4 -0.8
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -2.9 -0.2 +0.4 -0.3
stress Bacitracin 1 mg/ml N.D. -1.7 -0.0 -0.0 -1.1
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. -0.2 +0.8 -2.6 -0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.4 -1.1 +0.2 -1.3
stress Chloramphenicol 0.0025 mg/ml N.D. -0.4 -0.1 -0.7 -1.1
stress Benzalkonium Chloride 0.004 mg/ml N.D. +0.2 -0.1 -1.0 -1.4
stress LB Anaerobic with Spiramycin 0.00003125 mM N.D. -1.2 -0.2 -0.8 -0.2
nitrogen source Putrescine (N) N.D. +0.9 -2.7 -0.2 -0.2
carbon source carbon source D-Mannose 0.5mM N.D. +0.2 -0.2 -1.0 -1.2
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM N.D. -0.3 +0.5 -1.8 -0.5
carbon source carbon source D-Mannose 5mM N.D. +0.2 -0.8 -0.6 -0.9
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM N.D. -1.4 +0.2 -0.1 -0.7
nitrogen source Putrescine (N) N.D. +0.6 -2.0 -0.2 -0.0
stress Cisplatin 0.1 mg/ml N.D. -0.6 +0.3 -0.7 -0.6
phage T7_phage 0.00015625 MOI TC4 N.D. -0.6 -0.0 +0.8 -1.4
stress Ciprofloxacin Hydrochloride 0.0024 mM N.D. -0.1 +0.1 +0.5 -1.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. +0.3 -0.3 -1.4 +0.9
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.8 +0.2 +1.4 +0.3
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. -0.2 +0.3 -1.2 +2.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. -0.3 -0.4 +0.4 +1.5
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +0.1 +0.4 -2.2 +3.3
stress 2-nitrobenzenesulfonyl fluoride N.D. +0.3 -0.0 -0.9 +2.3
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