Fitness for 5 genes in Escherichia coli BW25113

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500 ntybjH and b0844 are separated by 85 nucleotidesb0844 and ybjJ overlap by 1 nucleotidesybjJ and ybjK are separated by 83 nucleotidesybjK and rybB are separated by 16 nucleotides b0843: ybjH - hypothetical protein (NCBI), at 884,169 to 884,453 ybjH b0844: b0844 - orf, hypothetical protein (VIMSS), at 884,539 to 885,354 b0844 b0845: ybjJ - predicted transporter (NCBI), at 885,354 to 886,562 ybjJ b0846: ybjK - predicted DNA-binding transcriptional regulator (NCBI), at 886,646 to 887,182 ybjK b4417: rybB - unknown RNA (NCBI), at 887,199 to 887,277 rybB
Group Condition b0843 b0844 b0845 b0846 b4417
ybjH b0844 ybjJ ybjK rybB
motility outer cut, LB soft agar motility assay -2.2 +0.1 -0.6 -0.8 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.4 -0.4 -1.5 N.D.
carbon source carbon source D-Mannose 1mM -1.5 +0.3 -0.3 -0.8 N.D.
nophagecontrol R1_N4_T1_phage0 -2.1 -0.2 +0.1 +0.1 N.D.
phage R2_N4_T1_phage10 -0.7 -0.7 -0.3 -0.3 N.D.
carbon source carbon source D-Glucose 20mM -1.9 +0.2 -0.0 -0.3 N.D.
carbon source carbon source D-Mannose 5mM -1.1 -0.3 -0.0 -0.6 N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 -0.7 -0.4 -0.8 N.D.
motility outer cut, LB soft agar motility assay -1.6 +0.1 +0.1 -0.4 N.D.
stress benzenesulfonyl fluoride -1.4 -0.3 -0.1 +0.2 N.D.
carbon source carbon source D-Glucose 0.25mM -0.9 +0.5 -0.5 -0.7 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -1.4 +0.4 +0.0 -0.5 N.D.
phage N4_phage 0.009375 MOI TC2 -2.3 +0.3 +0.1 +0.7 N.D.
phage T7_phage 0.0015625 MOI TC2 -1.5 -0.4 +0.6 +0.2 N.D.
phage R2_N4_T2_phage10 -1.8 -0.1 +0.5 +0.5 N.D.
msc 22hr_mediaonly_rep1_lowinoculum -1.3 +0.2 +0.5 +0.1 N.D.
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.2 -0.7 -3.1 +3.8 N.D.
carbon source carbon source D-Glucose 0.25mM +1.3 -0.2 +0.1 -1.5 N.D.
stress 1-ethyl-3-methylimidazolium acetate 60 mM -0.5 -0.2 -0.9 +1.3 N.D.
carbon source pyruvate (C) +0.5 -0.2 +0.4 -1.0 N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON +1.1 +0.1 -0.6 -0.7 N.D.
stress Cisplatin 0.1 mg/ml -0.4 -0.1 -0.9 +1.4 N.D.
nitrogen source Putrescine (N) +0.9 -0.1 +0.3 -1.1 N.D.
stress 1-ethyl-3-methylimidazolium acetate 30 mM -0.2 -0.3 -1.0 +1.9 N.D.
stress LB Anaerobic with Spectinomycin 0.0000625 mM -0.6 +0.6 +0.7 +0.7 N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.5 -0.7 -2.8 +5.8 N.D.
phage T7_phage 0.00015625 MOI TC4 +1.7 -0.1 -0.1 +0.4 N.D.
stress Benzalkonium Chloride 0.004 mg/ml +1.3 -0.8 -0.5 +1.9 N.D.
control PBS_cage_2_mouse_2_Cecum_40 hr +0.8 +0.4 +0.1 +0.8 N.D.
stress LB Anaerobic with Spectinomycin 0.0000625 mM +0.5 +0.6 +1.0 +0.8 N.D.
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