Fitness for 5 genes in Escherichia coli BW25113

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500 ntyliG and yliH are separated by 212 nucleotidesyliH and yliI are separated by 110 nucleotidesyliI and yliJ overlap by 4 nucleotidesyliJ and dacC are separated by 246 nucleotides b0835: yliG - predicted SAM-dependent methyltransferase (NCBI), at 875,933 to 877,258 yliG b0836: yliH - hypothetical protein (NCBI), at 877,471 to 877,854 yliH b0837: yliI - predicted dehydrogenase (NCBI), at 877,965 to 879,080 yliI b0838: yliJ - putative transferase (VIMSS), at 879,077 to 879,703 yliJ b0839: dacC - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a) (NCBI), at 879,950 to 881,152 dacC
Group Condition b0835 b0836 b0837 b0838 b0839
yliG yliH yliI yliJ dacC
stress Benzalkonium Chloride 0.004 mg/ml -0.4 -0.2 -1.6 +0.1 -0.9
phage Δdbn_10 -0.4 -1.3 -0.0 -0.1 -0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -3.1 -0.2 +0.4 +0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.3 -1.2 -0.2 -0.0 -0.8
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 -1.6 -0.2 -0.0 -0.5
phage Ste41 -0.7 -1.3 -0.3 -0.1 +0.5
stress LB Aerobic with Doxycycline hyclate 0.00000625 mM -0.3 -1.1 -0.6 -0.3 +0.4
carbon source carbon source D-Mannose 1mM -0.8 -1.0 -0.0 +0.3 +0.1
control PBS_cage_1_mice _Feces_16 hr +0.2 -1.0 -0.7 +0.1 -0.0
phage TP4 +0.2 -1.7 -0.1 +0.0 +0.3
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.3 -1.5 +0.4 -0.1 +0.3
phage Bas60 -0.3 -1.3 +0.2 +0.1 +0.1
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.4 -1.0 +0.4 -0.2 +0.3
stress Cephalothin 0.01 mg/ml -0.6 +1.0 -0.2 -0.6 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.6 -0.5 +0.1 +0.2 -0.8
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.7 -0.9 -0.2 -0.2 +0.6
stress LB Aerobic with Irgasan 0.0000003125 mM +0.7 +0.0 +0.6 -0.2 -0.9
stress Doxycycline hyclate 0.001 mg/ml -0.4 +0.8 -0.4 -0.3 +0.5
stress octanol 0.08 vol% -0.7 +0.9 -0.2 +0.4 -0.0
stress 2-nitrobenzenesulfonyl fluoride -0.5 +0.6 +0.5 +0.2 -0.2
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.6 +0.9 +0.2 -0.4 +0.5
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.4 -0.3 +0.5 +0.3 +0.4
stress LB Anaerobic with Cefoxitin 0.000003125 mM -0.4 +0.5 +0.2 -0.2 +0.6
motility outer cut, LB soft agar motility assay +0.3 +0.5 -0.0 +0.6 -0.7
nophagecontrol R1_N4_T1_phage0 +0.3 +1.1 +0.1 -0.3 -0.4
phage TP4 -0.4 +0.9 -0.2 +0.2 +0.3
control PBS_cage_2_mouse_2_Cecum_40 hr -0.2 -0.5 +1.0 +0.7 +0.2
phage T7_phage 0.00015625 MOI TC4 +0.5 +1.1 +0.2 -0.4 -0.0
carbon source carbon source D-Mannose 0.5mM -0.3 +0.9 +0.5 +0.3 +0.1
stress LB Anaerobic with Spectinomycin 0.0000625 mM -0.1 +0.6 +0.4 +0.5 +0.7
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