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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yliG and yliH are separated by 212 nucleotides
yliH and yliI are separated by 110 nucleotides
yliI and yliJ overlap by 4 nucleotides
yliJ and dacC are separated by 246 nucleotides
b0835: yliG - predicted SAM-dependent methyltransferase (NCBI), at 875,933 to 877,258
yliG
b0836: yliH - hypothetical protein (NCBI), at 877,471 to 877,854
yliH
b0837: yliI - predicted dehydrogenase (NCBI), at 877,965 to 879,080
yliI
b0838: yliJ - putative transferase (VIMSS), at 879,077 to 879,703
yliJ
b0839: dacC - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a) (NCBI), at 879,950 to 881,152
dacC
Group
Condition
b0835
b0836
b0837
b0838
b0839
yliG
yliH
yliI
yliJ
dacC
stress
Benzalkonium Chloride 0.004 mg/ml
-0.4
-0.2
-1.6
+0.1
-0.9
phage
Δdbn_10
-0.4
-1.3
-0.0
-0.1
-0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-3.1
-0.2
+0.4
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.3
-1.2
-0.2
-0.0
-0.8
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.3
-1.6
-0.2
-0.0
-0.5
phage
Ste41
-0.7
-1.3
-0.3
-0.1
+0.5
stress
LB Aerobic with Doxycycline hyclate 0.00000625 mM
-0.3
-1.1
-0.6
-0.3
+0.4
carbon source
carbon source D-Mannose 1mM
-0.8
-1.0
-0.0
+0.3
+0.1
control
PBS_cage_1_mice _Feces_16 hr
+0.2
-1.0
-0.7
+0.1
-0.0
phage
TP4
+0.2
-1.7
-0.1
+0.0
+0.3
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.3
-1.5
+0.4
-0.1
+0.3
phage
Bas60
-0.3
-1.3
+0.2
+0.1
+0.1
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.4
-1.0
+0.4
-0.2
+0.3
stress
Cephalothin 0.01 mg/ml
-0.6
+1.0
-0.2
-0.6
-0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.6
-0.5
+0.1
+0.2
-0.8
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.7
-0.9
-0.2
-0.2
+0.6
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.7
+0.0
+0.6
-0.2
-0.9
stress
Doxycycline hyclate 0.001 mg/ml
-0.4
+0.8
-0.4
-0.3
+0.5
stress
octanol 0.08 vol%
-0.7
+0.9
-0.2
+0.4
-0.0
stress
2-nitrobenzenesulfonyl fluoride
-0.5
+0.6
+0.5
+0.2
-0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.6
+0.9
+0.2
-0.4
+0.5
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.4
-0.3
+0.5
+0.3
+0.4
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
-0.4
+0.5
+0.2
-0.2
+0.6
motility
outer cut, LB soft agar motility assay
+0.3
+0.5
-0.0
+0.6
-0.7
nophagecontrol
R1_N4_T1_phage0
+0.3
+1.1
+0.1
-0.3
-0.4
phage
TP4
-0.4
+0.9
-0.2
+0.2
+0.3
control
PBS_cage_2_mouse_2_Cecum_40 hr
-0.2
-0.5
+1.0
+0.7
+0.2
phage
T7_phage 0.00015625 MOI TC4
+0.5
+1.1
+0.2
-0.4
-0.0
carbon source
carbon source D-Mannose 0.5mM
-0.3
+0.9
+0.5
+0.3
+0.1
stress
LB Anaerobic with Spectinomycin 0.0000625 mM
-0.1
+0.6
+0.4
+0.5
+0.7
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