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Cofit
Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
bioB and bioF overlap by 4 nucleotides
bioF and bioC overlap by 14 nucleotides
bioC and bioD overlap by 8 nucleotides
bioD and uvrB are separated by 578 nucleotides
b0775: bioB - biotin synthase (NCBI), at 808,567 to 809,607
bioB
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
b0779: uvrB - excinuclease ABC subunit B (NCBI), at 812,749 to 814,770
uvrB
Group
Condition
b0775
b0776
b0777
b0778
b0779
bioB
bioF
bioC
bioD
uvrB
carbon source
D-Glucose (C) MOPS
-2.6
-2.2
-1.3
-2.6
+0.0
carbon source
D-Glucose (C) MOPS
-3.3
-1.5
-1.7
-2.1
+0.0
carbon source
succinate (C)
-3.2
-2.0
-1.1
-1.8
-0.1
carbon source
L-Malic (C)
-1.6
-1.7
-1.9
-2.0
-0.1
carbon source
D-Galacturonic Acid (C) MOPS
-2.4
-1.3
-1.7
-1.9
+0.1
carbon source
D-Galacturonic Acid (C) MOPS
-2.4
-1.5
-1.6
-1.8
+0.3
carbon source
L-Malic (C)
-2.1
-1.3
-1.7
-1.7
+0.1
carbon source
succinate (C)
-2.0
-1.5
-1.3
-1.6
-0.0
carbon source
Glycolic Acid (C)
-1.8
-1.0
-1.7
-1.2
-0.3
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.4
+0.3
-0.7
-1.0
-5.0
stress
Cisplatin 0.1 mg/ml
+0.1
+0.1
+0.3
-0.7
-5.6
stress
Cisplatin 0.1 mg/ml
-0.2
-0.2
+0.1
+0.0
-5.5
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.0
-0.6
+0.2
-0.8
-4.5
stress
Cisplatin 0.1 mg/ml
-0.0
+0.2
+0.2
-0.2
-5.5
carbon source
Glycolic Acid (C)
-1.8
-1.0
-0.7
-1.3
-0.3
nutrient
M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin
-1.3
-1.1
-1.0
-1.6
-0.1
stress
2-nitrobenzenesulfonyl fluoride
-0.3
-0.1
-0.8
-0.1
-3.9
nitrogen source
casaminos (N)
-1.6
-0.9
-0.9
-1.2
-0.0
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.2
-0.4
-0.1
-0.1
-4.2
stress
Cisplatin 0.1 mg/ml
+0.5
+0.2
+0.5
+0.1
-5.9
nutrient
M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin
-1.0
-1.1
-1.0
-1.1
+0.1
nutrient
M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin
-1.1
-1.2
-0.9
-1.0
+0.1
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.4
+0.1
-0.4
+0.0
-3.9
stress
Cisplatin 0.05 mg/ml
-0.1
+0.1
+0.4
+0.0
-4.2
stress
Cisplatin 0.05 mg/ml
+0.1
+0.0
+0.5
-0.1
-3.8
stress
Cisplatin 0.05 mg/ml
-0.1
-0.3
+0.4
-0.3
-2.9
stress
Cisplatin 0.025 mg/ml
-0.2
-0.1
+0.5
+0.5
-3.7
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.4
-0.0
+0.5
+0.2
-4.1
stress
Cisplatin 0.05 mg/ml
+0.1
+0.3
+0.3
-0.2
-3.2
stress
Cisplatin 0.1 mg/ml
+0.6
-0.4
+0.1
+0.9
-3.6
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