Fitness for 5 genes in Escherichia coli BW25113

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500 ntmodE and ybhT are separated by 128 nucleotidesybhT and modA are separated by 166 nucleotidesmodA and modB overlap by 1 nucleotidesmodB and modC are separated by 2 nucleotides b0761: modE - DNA-binding transcriptional dual regulator (NCBI), at 793,079 to 793,867 modE b0762: ybhT - hypothetical protein (NCBI), at 793,996 to 794,145 ybhT b0763: modA - molybdate transporter subunit (NCBI), at 794,312 to 795,085 modA b0764: modB - molybdate ABC transporter permease protein (NCBI), at 795,085 to 795,774 modB b0765: modC - molybdate transporter subunit (NCBI), at 795,777 to 796,835 modC
Group Condition b0761 b0762 b0763 b0764 b0765
modE ybhT modA modB modC
phage RB68 -3.0 N.D. -2.9 -3.3 -3.0
stress 2,4-Dinitrophenol 2 mM -0.9 N.D. -2.9 -3.7 -1.8
stress 2,4-Dinitrophenol 2 mM -1.1 N.D. -3.0 -2.5 -2.1
phage RB68 -1.9 N.D. -1.7 -2.9 -1.7
stress LB Anaerobic with Chloramphenicol 0.000001953125 mM -1.3 N.D. -2.1 -2.7 -1.5
stress octanol 0.08 vol% -1.2 N.D. -0.6 -4.3 -1.2
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.9 N.D. -2.2 -2.0 -2.0
stress LB Anaerobic with Doxycycline hyclate 0.000000390625 mM -1.0 N.D. -1.4 -2.0 -1.7
phage Ste41 -0.8 N.D. -1.7 -2.2 -1.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.7 N.D. -3.0 -1.0 -0.8
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.7 N.D. -1.4 -1.1 -2.0
stress LB Aerobic with Irgasan 0.0000003125 mM -1.0 N.D. -1.3 -1.0 -1.7
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.8 N.D. -1.2 -1.9 -1.2
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM -0.5 N.D. -1.3 -1.3 -2.0
lb LB, Anaerobic -0.8 N.D. -1.5 -1.8 -0.8
stress LB Anaerobic with Trimethoprim 0.00000125 mM -0.8 N.D. -1.1 -1.6 -1.2
lb anaerobic no stress control LB anaerobic no stress control -0.7 N.D. -1.0 -1.4 -1.4
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM -0.9 N.D. -1.2 -1.3 -1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.2 N.D. +0.9 +1.5 +0.9
stress Cisplatin 0.1 mg/ml +0.7 N.D. +1.3 +1.2 +1.3
stress Spectinomycin 0.00625 mg/ml +0.6 N.D. +1.5 +1.5 +1.1
stress 1-ethyl-3-methylimidazolium chloride 40 mM +0.8 N.D. +1.3 +1.6 +1.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.9 N.D. +1.2 +1.5 +1.2
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.5 N.D. +1.5 +1.6 +1.5
motility outer cut, LB soft agar motility assay +0.4 N.D. +1.9 +1.5 +1.8
stress Benzalkonium Chloride 0.004 mg/ml +0.8 N.D. +1.4 +1.8 +1.7
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.1 N.D. +1.7 +1.6 +1.6
stress Spectinomycin 0.0125 mg/ml +1.0 N.D. +2.2 +2.2 +1.9
motility outer cut, LB soft agar motility assay +1.1 N.D. +2.5 +2.5 +2.4
motility outer cut, LB soft agar motility assay +1.6 N.D. +2.1 +2.3 +2.5
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