Fitness for 5 genes in Escherichia coli BW25113

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500 ntgalM and galK overlap by 7 nucleotidesgalK and galT are separated by 3 nucleotidesgalT and galE are separated by 9 nucleotidesgalE and modF are separated by 260 nucleotides b0756: galM - galactose-1-epimerase (mutarotase) (NCBI), at 787,020 to 788,060 galM b0757: galK - galactokinase GalK (EC 2.7.1.6) (from data), at 788,054 to 789,202 galK b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252 galT b0759: galE - UDP-galactose-4-epimerase (VIMSS), at 790,262 to 791,278 galE b0760: modF - fused molybdate transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 791,539 to 793,011 modF
Group Condition b0756 b0757 b0758 b0759 b0760
galM galK galT galE modF
carbon source D-Galactose (C) -0.2 -4.8 -6.0 -5.1 +0.2
carbon source D-Galactose (C) -0.5 -4.6 -5.9 -4.8 -0.1
carbon source D-Galacturonic Acid (C) M9 -0.6 -1.1 -3.1 -2.6 -0.3
carbon source D-Galacturonic Acid (C) MOPS -0.3 -0.8 -2.3 -2.3 -0.1
carbon source D-Galacturonic Acid (C) -0.3 -0.7 -2.3 -2.5 +0.1
carbon source D-Galacturonic Acid (C) -0.4 -0.5 -2.0 -2.6 +0.0
stress octanol 0.08 vol% +0.5 -0.4 +0.2 -5.7 +0.1
stress Benzalkonium Chloride 0.004 mg/ml -0.2 -0.1 -1.7 -3.1 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -0.1 -0.5 -4.3 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -0.0 -0.6 -3.2 +0.0
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM +0.2 -0.0 +0.0 -3.9 +0.0
carbon source carbon source D-Mannose 20mM +0.2 -0.4 -1.9 -1.4 -0.1
carbon source carbon source D-Mannose 1mM -0.3 -0.2 -1.3 -2.0 +0.3
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM +0.5 -0.1 +0.2 -4.2 +0.1
TN_15CS_15C_10dbl_rep3 +0.0 +0.1 -1.1 -2.3 -0.0
carbon source carbon source D-Mannose 20mM -0.5 +0.2 -2.1 -1.0 +0.1
carbon source D-Mannose (C) +0.0 -0.0 -1.6 -1.6 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 +0.1 -0.0 -3.0 -0.2
TN_15CS_warm37C_5dbl_rep1 +0.2 +0.0 -1.2 -2.3 -0.0
control PBS_cage_2_mouse_2_Cecum_40 hr -0.3 +0.1 -0.5 -2.2 -0.3
TN_15CS_warm37C_5dbl_rep2 -0.0 +0.2 -1.0 -2.1 -0.1
TN_15CS_15C_10dbl_rep2 -0.0 +0.2 -1.0 -2.0 -0.0
TN_15CS_warm37C_5dbl_rep3 +0.1 +0.2 -1.0 -2.0 -0.1
TN_15CS_15C_10dbl_rep1 +0.2 +0.1 -1.1 -1.9 -0.0
carbon source carbon source D-Mannose 5mM +1.0 +0.1 -2.4 -1.7 +0.4
carbon source carbon source D-Mannose 1mM +0.5 -0.0 -1.5 -1.4 +0.2
phage Ste41 -2.3 +0.6 -1.0 +1.3 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.1 -0.4 +3.0 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.3 -0.4 +3.1 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.2 +0.0 +3.0 +0.4
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