Fitness for 5 genes in Escherichia coli BW25113

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500 ntnei and abrB overlap by 4 nucleotidesabrB and ybgO are separated by 151 nucleotidesybgO and ybgP overlap by 4 nucleotidesybgP and ybgQ are separated by 14 nucleotides b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949 nei b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992 abrB b0716: ybgO - orf, hypothetical protein (VIMSS), at 747,144 to 748,205 ybgO b0717: ybgP - predicted assembly protein (NCBI), at 748,202 to 748,930 ybgP b0718: ybgQ - putative outer membrane protein (VIMSS), at 748,945 to 751,392 ybgQ
Group Condition b0714 b0715 b0716 b0717 b0718
nei abrB ybgO ybgP ybgQ
carbon source carbon source D-Glucose 5mM -2.0 -0.2 -0.2 -0.2 -0.0
phage Ste41 -1.4 -0.3 +0.4 -0.3 -0.2
phage T7_phage 0.00015625 MOI TC4 -1.8 +0.2 +0.1 -0.0 +0.0
phage N4_phage 0.009375 MOI TC2 -0.2 -0.2 -0.3 -0.6 -0.1
phage N4_phage 0.09375 MOI TC1 -0.2 -0.3 -0.3 -0.5 -0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.2 -0.3 -0.1 -0.6 -0.1
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -0.4 +0.3 -0.6 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.5 -0.2 -0.4 +0.1 -0.2
phage T7_phage 0.0015625 MOI TC2 -1.0 -0.3 +0.3 -0.0 -0.1
carbon source carbon source D-Mannose 1mM +0.4 -0.7 +0.1 -0.8 +0.1
carbon source carbon source D-Glucose 0.25mM +0.5 -0.4 -0.3 -0.2 -0.2
phage Δdbn_10 -0.5 -0.0 -0.4 +0.5 +0.0
stress LB Aerobic with Ramoplanin 0.000125 mM +0.0 +0.6 -0.3 -0.5 -0.0
control PBS_cage_2_mouse_1_Cecum_40 hr +0.5 +0.1 -0.3 -0.9 +0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.9 -0.5 -0.3 -0.2 +0.1
control PBS_cage_1_mice _Feces_40 hr +0.3 +0.3 +0.2 -0.8 -0.0
control PBS_cage_1_mouse_2_Cecum_40 hr +0.9 -0.2 -0.1 -0.7 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -0.4 +0.5 -0.3 +0.3
stress Cisplatin 0.1 mg/ml +0.8 +0.3 -0.9 +0.1 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 -0.2 -0.5 +0.5 -0.2
phage R2_N4_T2_phage10 -0.6 +0.1 +0.2 +0.3 +0.3
control PBS_cage_1_mice _Feces_16 hr +0.5 +0.5 -0.0 -0.5 -0.0
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.6 +0.5 -0.1 -0.2 +0.1
protonated/deuterated deuterated glucose in H2O +0.3 -0.3 +0.2 +0.5 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.3 +0.5 +0.6 +0.1 +0.1
motility outer cut, LB soft agar motility assay +1.1 +0.2 +0.0 -0.2 +0.0
control NoBALOControl +0.9 +0.1 +0.2 +0.2 -0.2
stress Benzalkonium Chloride 0.004 mg/ml +0.9 +0.5 -0.1 -0.1 +0.1
motility inner cut, LB soft agar motility assay +0.6 +0.2 -0.1 +0.6 +0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.7 +0.5 +0.1 +0.4 +0.2
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