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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
phr and ybgH are separated by 149 nucleotides
ybgH and ybgI are separated by 270 nucleotides
ybgI and ybgJ are separated by 22 nucleotides
ybgJ and ybgK overlap by 7 nucleotides
b0708: phr - deoxyribodipyrimidine photolyase, FAD-binding (NCBI), at 738,730 to 740,148
phr
b0709: ybgH - predicted transporter (NCBI), at 740,298 to 741,779
ybgH
b0710: ybgI - conserved metal-binding protein (NCBI), at 742,050 to 742,793
ybgI
b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472
ybgJ
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
Group
Condition
b0708
b0709
b0710
b0711
b0712
phr
ybgH
ybgI
ybgJ
ybgK
phage
T7_phage 0.0015625 MOI TC2
-0.4
-0.3
-0.6
-0.3
-1.4
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.0
-0.0
-1.4
-0.8
-0.2
stress
Cisplatin 0.1 mg/ml
-1.1
+0.2
-1.1
+0.0
+0.4
stress
2-Furfuraldehyde 0.25 vol%
+0.0
+0.0
-1.5
-0.3
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.1
-1.5
-0.1
+0.1
phage
T7_phage 0.00015625 MOI TC4
-0.5
+0.0
+0.6
-0.8
-0.3
phage
Δdbn_10
-0.3
+0.5
+0.2
-0.3
-1.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.4
+0.3
-0.7
-0.3
+0.4
phage
RB68
+0.2
-0.2
-0.7
-0.3
+0.1
carbon source
acetate (C)
-0.2
-0.1
-0.9
+0.4
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
+0.1
-1.0
+0.1
+0.3
carbon source
carbon source D-Glucose 0.25mM
+0.6
+0.1
-0.8
-0.0
-0.3
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.4
+0.1
-2.0
+0.6
+0.5
carbon source
Dodecanol and Rhamnolipid (C)
-0.5
-0.4
+0.6
-0.5
+0.5
stress
Aluminum chloride 5 mM
+0.4
-0.3
-0.2
-0.4
+0.3
motility
inner cut, LB soft agar motility assay
+0.1
+0.2
-0.7
-0.1
+0.6
motility
inner cut, LB soft agar motility assay
-0.1
-0.1
-0.6
+0.3
+0.6
control
PBS_cage_1_mice _Feces_40 hr
-0.2
+0.2
-0.4
+0.9
+0.0
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.7
+0.2
+0.7
-0.6
-0.3
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.4
-0.1
+0.4
-0.4
+0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.5
+0.1
+0.2
+0.8
+0.2
control
PBS_cage_1_mice _Feces_16 hr
-0.5
-0.0
+0.0
+1.2
+0.1
control
PBS_cage_1_mice _Feces_22 hr
-0.0
+0.3
-0.2
+1.0
-0.1
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.3
+0.1
+0.7
+0.1
+0.3
phage
Ste41
-0.1
-0.2
+0.6
+0.3
+0.5
carbon source
carbon source D-Mannose 5mM
+0.2
-0.3
+0.6
+0.7
+0.1
control
PBS_cage_2_mouse_2_Cecum_40 hr
-0.3
+0.2
+0.6
+0.2
+0.8
stress
Trimethoprim 0.00125 mM
-0.1
+0.1
+0.7
+0.6
+0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.2
-0.2
+0.5
+0.8
+0.5
carbon source
carbon source D-Glucose 5mM
+0.7
+0.2
+0.9
+0.5
+0.8
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