Fitness for 5 genes in Escherichia coli BW25113

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500 ntphr and ybgH are separated by 149 nucleotidesybgH and ybgI are separated by 270 nucleotidesybgI and ybgJ are separated by 22 nucleotidesybgJ and ybgK overlap by 7 nucleotides b0708: phr - deoxyribodipyrimidine photolyase, FAD-binding (NCBI), at 738,730 to 740,148 phr b0709: ybgH - predicted transporter (NCBI), at 740,298 to 741,779 ybgH b0710: ybgI - conserved metal-binding protein (NCBI), at 742,050 to 742,793 ybgI b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472 ybgJ b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398 ybgK
Group Condition b0708 b0709 b0710 b0711 b0712
phr ybgH ybgI ybgJ ybgK
phage T7_phage 0.0015625 MOI TC2 -0.4 -0.3 -0.6 -0.3 -1.4
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.0 -0.0 -1.4 -0.8 -0.2
stress Cisplatin 0.1 mg/ml -1.1 +0.2 -1.1 +0.0 +0.4
stress 2-Furfuraldehyde 0.25 vol% +0.0 +0.0 -1.5 -0.3 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.1 -1.5 -0.1 +0.1
phage T7_phage 0.00015625 MOI TC4 -0.5 +0.0 +0.6 -0.8 -0.3
phage Δdbn_10 -0.3 +0.5 +0.2 -0.3 -1.0
stress LB Aerobic with Irgasan 0.0000003125 mM -0.4 +0.3 -0.7 -0.3 +0.4
phage RB68 +0.2 -0.2 -0.7 -0.3 +0.1
carbon source acetate (C) -0.2 -0.1 -0.9 +0.4 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 +0.1 -1.0 +0.1 +0.3
carbon source carbon source D-Glucose 0.25mM +0.6 +0.1 -0.8 -0.0 -0.3
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 +0.1 -2.0 +0.6 +0.5
carbon source Dodecanol and Rhamnolipid (C) -0.5 -0.4 +0.6 -0.5 +0.5
stress Aluminum chloride 5 mM +0.4 -0.3 -0.2 -0.4 +0.3
motility inner cut, LB soft agar motility assay +0.1 +0.2 -0.7 -0.1 +0.6
motility inner cut, LB soft agar motility assay -0.1 -0.1 -0.6 +0.3 +0.6
control PBS_cage_1_mice _Feces_40 hr -0.2 +0.2 -0.4 +0.9 +0.0
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.7 +0.2 +0.7 -0.6 -0.3
control PBS_cage_1_mouse_1_Cecum_40 hr +0.4 -0.1 +0.4 -0.4 +0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 +0.1 +0.2 +0.8 +0.2
control PBS_cage_1_mice _Feces_16 hr -0.5 -0.0 +0.0 +1.2 +0.1
control PBS_cage_1_mice _Feces_22 hr -0.0 +0.3 -0.2 +1.0 -0.1
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.3 +0.1 +0.7 +0.1 +0.3
phage Ste41 -0.1 -0.2 +0.6 +0.3 +0.5
carbon source carbon source D-Mannose 5mM +0.2 -0.3 +0.6 +0.7 +0.1
control PBS_cage_2_mouse_2_Cecum_40 hr -0.3 +0.2 +0.6 +0.2 +0.8
stress Trimethoprim 0.00125 mM -0.1 +0.1 +0.7 +0.6 +0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -0.2 +0.5 +0.8 +0.5
carbon source carbon source D-Glucose 5mM +0.7 +0.2 +0.9 +0.5 +0.8
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