Fitness for 5 genes in Escherichia coli BW25113

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500 ntybfF and seqA are separated by 184 nucleotidesseqA and pgm are separated by 25 nucleotidespgm and ybfP are separated by 213 nucleotidesybfP and ybfG are separated by 40 nucleotides b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF b0687: seqA - regulatory protein for replication initiation (NCBI), at 712,210 to 712,755 seqA b0688: pgm - phosphoglucomutase (NCBI), at 712,781 to 714,421 pgm b0689: ybfP - hypothetical protein (NCBI), at 714,635 to 715,129 ybfP b0691: ybfG - orf, hypothetical protein (VIMSS), at 715,170 to 715,820 ybfG
Group Condition b0686 b0687 b0688 b0689 b0691
ybfF seqA pgm ybfP ybfG
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.8 N.D. -6.8 -0.0 +0.2
control PBS_cage_2_mouse_2_Cecum_40 hr -0.5 N.D. -6.3 -0.2 -0.2
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr +0.1 N.D. -6.9 -0.2 +0.3
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr -0.3 N.D. -5.9 -0.2 -0.1
control PBS_cage_1_mouse_2_Cecum_40 hr -0.0 N.D. -6.6 +0.1 +0.3
control PBS_cage_2_mouse_1_Cecum_40 hr +0.1 N.D. -6.2 +0.1 -0.1
phage Phage_N4_cage_4_mice _Feces_40 hr +0.0 N.D. -6.1 -0.1 +0.1
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.0 N.D. -6.5 +0.3 +0.4
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr -0.3 N.D. -5.1 -0.0 -0.2
stress octanol 0.08 vol% -0.3 N.D. -4.6 -0.5 -0.2
control PBS_cage_2_mouse_3_Cecum_40 hr -0.3 N.D. -4.9 -0.2 +0.1
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr +0.1 N.D. -5.3 -0.1 +0.1
control PBS_cage_2_mice _Feces_40 hr -0.1 N.D. -5.3 +0.1 +0.1
phage T7_phage 0.000015625 MOI TC4 +0.0 N.D. -5.1 -0.1 +0.0
phage Phage_N4_cage_3_mice _Feces_40 hr -0.4 N.D. -4.9 +0.1 +0.2
control PBS_cage_2_mice _Feces_22 hr -0.2 N.D. -4.7 -0.0 -0.0
motility outer cut, LB soft agar motility assay -0.5 N.D. -4.5 -0.1 +0.1
control PBS_cage_1_mice _Feces_40 hr +0.2 N.D. -4.9 -0.0 +0.1
stress decyl alcohol 5 vol% +0.0 N.D. -4.7 +0.2 -0.0
phage T7_phage 0.00015625 MOI TC2 -0.2 N.D. -4.5 +0.1 +0.2
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.1 N.D. -4.5 +0.3 -0.4
stress LB Aerobic with Irgasan 0.0000003125 mM -3.7 N.D. -1.2 +0.0 +0.6
stress hexanol 0.31 vol% -0.5 N.D. -4.1 +0.3 +0.1
control PBS_cage_1_mice _Feces_22 hr +0.5 N.D. -4.3 +0.3 +0.5
control PBS_cage_1_mouse_1_Cecum_40 hr +0.8 N.D. -3.9 +0.2 +0.0
phage RB68 -0.7 N.D. +3.9 +0.0 +0.3
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.2 N.D. +4.9 -0.3 -0.1
stress Bacitracin 1 mg/ml +0.9 N.D. +3.9 +0.2 -0.1
phage Ste41 -0.7 N.D. +5.4 +0.1 +0.2
phage Ste41 -0.3 N.D. +5.6 -0.0 +0.5
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