Fitness for 5 genes in Escherichia coli BW25113

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500 ntfldA and ybfE are separated by 139 nucleotidesybfE and ybfF are separated by 139 nucleotidesybfF and seqA are separated by 184 nucleotidesseqA and pgm are separated by 25 nucleotides b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688 fldA b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121 ybfE b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025 ybfF b0687: seqA - regulatory protein for replication initiation (NCBI), at 712,210 to 712,755 seqA b0688: pgm - phosphoglucomutase (NCBI), at 712,781 to 714,421 pgm
Group Condition b0684 b0685 b0686 b0687 b0688
fldA ybfE ybfF seqA pgm
motility outer cut, LB soft agar motility assay N.D. -6.9 +0.0 N.D. -3.8
stress Cisplatin 0.1 mg/ml N.D. -5.4 -0.1 N.D. -2.7
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr N.D. +0.0 -0.8 N.D. -6.8
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. -0.0 +0.0 N.D. -6.5
stress octanol 0.08 vol% N.D. -1.2 -0.3 N.D. -4.6
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr N.D. +0.6 +0.1 N.D. -6.9
control PBS_cage_1_mouse_2_Cecum_40 hr N.D. +0.8 -0.0 N.D. -6.6
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. +0.9 -0.5 N.D. -6.3
phage Lambda_ci1857 N.D. -5.1 -0.3 N.D. -0.3
control PBS_cage_2_mouse_3_Cecum_40 hr N.D. -0.5 -0.3 N.D. -4.9
phage T7_phage 0.0015625 MOI TC1 N.D. -1.8 +0.0 N.D. -3.8
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr N.D. +0.7 -0.3 N.D. -5.9
control PBS_cage_2_mouse_1_Cecum_40 hr N.D. +0.8 +0.1 N.D. -6.2
phage Phage_N4_cage_4_mice _Feces_40 hr N.D. +0.8 +0.0 N.D. -6.1
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr N.D. +0.2 -0.3 N.D. -5.1
stress Benzalkonium Chloride 0.004 mg/ml N.D. -3.6 +1.1 N.D. -2.3
control PBS_cage_1_mice _Feces_40 hr N.D. +0.8 +0.2 N.D. -4.9
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM N.D. -3.8 -0.7 N.D. +1.3
control PBS_cage_1_mice _Feces_22 hr N.D. +0.7 +0.5 N.D. -4.3
control PBS_cage_1_mouse_1_Cecum_40 hr N.D. +0.9 +0.8 N.D. -3.9
drt_type_3 DRT_type_3 N.D. +1.5 +0.4 N.D. -3.9
retron_ec_48_control retron_Ec_48_control N.D. +1.9 +0.4 N.D. -4.1
phage RB68 N.D. -1.1 -0.7 N.D. +3.9
phage Ste41 N.D. -2.2 -0.7 N.D. +5.4
phage Ste41 N.D. -1.5 -0.3 N.D. +5.6
stress Trimethoprim 0.00125 mM N.D. +6.7 +0.5 N.D. -2.5
stress 2-Furfuraldehyde 0.25 vol% N.D. +4.0 +1.1 N.D. +0.6
stress Bacitracin 1 mg/ml N.D. +2.3 +0.9 N.D. +3.9
stress Trimethoprim 0.0025 mM N.D. +8.1 +0.6 N.D. -0.2
stress Trimethoprim 0.005 mM N.D. +8.4 +0.7 N.D. +0.8
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