Fitness for 5 genes in Escherichia coli BW25113

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500 ntnagA and nagB are separated by 59 nucleotidesnagB and nagE are separated by 332 nucleotidesnagE and glnS are separated by 202 nucleotidesglnS and ybfM are separated by 576 nucleotides b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974 nagA b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834 nagB b0679: nagE - fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB , and IIA components (NCBI), at 703,167 to 705,113 nagE b0680: glnS - glutaminyl-tRNA synthetase (NCBI), at 705,316 to 706,980 glnS b0681: ybfM - predicted outer membrane porin (NCBI), at 707,557 to 708,963 ybfM
Group Condition b0677 b0678 b0679 b0680 b0681
nagA nagB nagE glnS ybfM
carbon source Ying_sugar7 rep C; time point 3 -5.0 -8.7 -0.1 N.D. +0.1
carbon source Ying_sugar7 rep B; time point 3 -4.2 -8.9 +0.1 N.D. +0.0
carbon source Ying_sugar7 rep A; time point 3 -3.8 -8.8 +0.1 N.D. -0.0
carbon source Ying_sugar7 rep B; time point 2 -2.7 -9.0 +0.1 N.D. -0.0
carbon source NAG (C) -4.8 -5.2 -1.1 N.D. +0.1
carbon source Ying_sugar7 rep C; time point 2 -2.9 -8.1 -0.1 N.D. +0.0
carbon source NAG (C) -4.6 -4.8 -1.2 N.D. -0.0
carbon source Ying_sugar7 rep A; time point 2 -2.3 -6.6 +0.1 N.D. +0.0
carbon source Ying_sugar7 rep B; time point 1 -1.4 -5.8 +0.1 N.D. +0.0
carbon source Ying_sugar7 rep A; time point 1 -1.7 -4.0 -0.0 N.D. -0.0
carbon source Ying_all64 rep C; time point 3 -2.4 -3.3 +0.0 N.D. -0.0
carbon source Ying_all64 rep A; time point 3 -2.5 -3.1 +0.1 N.D. -0.2
carbon source Ying_all64 rep B; time point 3 -2.4 -3.0 +0.1 N.D. -0.0
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -3.9 -1.4 +0.2 N.D. +0.2
carbon source Ying_sugar7 rep C; time point 1 -1.4 -3.1 +0.0 N.D. -0.1
carbon source D-Glucosamine Hydrochloride (C) +1.7 -5.6 -0.4 N.D. -0.2
control PBS_cage_2_mouse_2_Cecum_40 hr -2.3 -1.8 +0.1 N.D. -0.5
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -3.4 -1.2 +0.2 N.D. +0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON -4.8 +0.3 +0.1 N.D. +0.2
carbon source D-Glucosamine Hydrochloride (C) +1.4 -4.8 -0.6 N.D. -0.1
stress LB Anaerobic with Spiramycin 0.00003125 mM -3.7 +0.4 -0.4 N.D. -0.1
stress undecene 2.5 vol% -3.8 +0.3 +0.1 N.D. -0.1
stress octanol 0.08 vol% -2.6 -1.1 +0.4 N.D. -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -3.6 +1.2 -0.8 N.D. +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -3.1 +1.2 -1.1 N.D. -0.1
stress LB Anaerobic with Irgasan 0.000000625 mM -3.6 +1.0 -0.5 N.D. +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -3.7 +0.8 +0.0 N.D. +0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -3.8 +1.1 -0.1 N.D. +0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -3.3 +1.1 -0.5 N.D. +0.3
stress LB Aerobic with Irgasan 0.0000003125 mM -2.5 +2.9 -1.0 N.D. +0.2
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