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Protein
Homologs
Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ybeY and ybeZ overlap by 4 nucleotides
ybeZ and miaB are separated by 152 nucleotides
miaB and ubiF are separated by 145 nucleotides
ubiF and glnX are separated by 153 nucleotides
b0659: ybeY - hypothetical protein (NCBI), at 691,097 to 691,564
ybeY
b0660: ybeZ - predicted protein with nucleoside triphosphate hydrolase domain (RefSeq), at 691,561 to 692,601
ybeZ
b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178
miaB
b0662: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI), at 694,324 to 695,499
ubiF
b0664: glnX - tRNA-Gln (NCBI), at 695,653 to 695,727
glnX
Group
Condition
b0659
b0660
b0661
b0662
b0664
ybeY
ybeZ
miaB
ubiF
glnX
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
-0.3
-1.9
N.D.
N.D.
phage
TP4
N.D.
-2.0
-0.1
N.D.
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
-0.5
-1.4
N.D.
N.D.
phage
T7_phage 0.00015625 MOI TC4
N.D.
-0.4
-1.5
N.D.
N.D.
phage
T7_phage 0.0015625 MOI TC1
N.D.
-0.2
-1.6
N.D.
N.D.
stress
LB Anaerobic with Furaltadone 0.000015625 mM
N.D.
-1.4
-0.4
N.D.
N.D.
nitrogen source
Putrescine (N)
N.D.
-0.8
-1.0
N.D.
N.D.
control
PBS_cage_1_mice _Feces_16 hr
N.D.
-1.4
-0.3
N.D.
N.D.
phage
T7_phage 0.00015625 MOI TC2
N.D.
-0.0
-1.7
N.D.
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+0.3
-1.9
N.D.
N.D.
phage
Δdbn_10
N.D.
-1.4
-0.1
N.D.
N.D.
phage
Ste41
N.D.
-1.4
-0.0
N.D.
N.D.
carbon source
Glycolic Acid (C)
N.D.
-1.1
-0.3
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
N.D.
-1.6
+0.3
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
N.D.
-1.5
+0.3
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
N.D.
-1.5
+0.3
N.D.
N.D.
plasmid transformation
mariner vector transformation; liquid outgrowth assay
N.D.
+0.1
-1.2
N.D.
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+0.2
-1.2
N.D.
N.D.
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
N.D.
-1.2
+0.3
N.D.
N.D.
stress
Nickel (II) chloride 1 mM
N.D.
-1.2
+0.5
N.D.
N.D.
carbon source
carbon source D-Glucose 5mM
N.D.
-1.0
+0.4
N.D.
N.D.
stress
Cephalothin 0.01 mg/ml
N.D.
-1.2
+0.7
N.D.
N.D.
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
N.D.
+0.8
-1.2
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
N.D.
+1.0
-1.1
N.D.
N.D.
stress
Cisplatin 0.1 mg/ml
N.D.
+1.0
-0.7
N.D.
N.D.
control
NoPredeControl
N.D.
-0.6
+0.9
N.D.
N.D.
carbon source
Adenosine 0.3742 mM (C)
N.D.
-0.5
+0.8
N.D.
N.D.
carbon source
Acetate (C)
N.D.
-0.4
+1.1
N.D.
N.D.
carbon source
Ying_sugar7 rep A; time point 3
N.D.
-0.2
+1.1
N.D.
N.D.
carbon source
L-Malic (C)
N.D.
+0.5
+0.8
N.D.
N.D.
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