Fitness for 5 genes in Escherichia coli BW25113

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500 ntybeY and ybeZ overlap by 4 nucleotidesybeZ and miaB are separated by 152 nucleotidesmiaB and ubiF are separated by 145 nucleotidesubiF and glnX are separated by 153 nucleotides b0659: ybeY - hypothetical protein (NCBI), at 691,097 to 691,564 ybeY b0660: ybeZ - predicted protein with nucleoside triphosphate hydrolase domain (RefSeq), at 691,561 to 692,601 ybeZ b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178 miaB b0662: ubiF - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI), at 694,324 to 695,499 ubiF b0664: glnX - tRNA-Gln (NCBI), at 695,653 to 695,727 glnX
Group Condition b0659 b0660 b0661 b0662 b0664
ybeY ybeZ miaB ubiF glnX
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.3 -1.9 N.D. N.D.
phage TP4 N.D. -2.0 -0.1 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.5 -1.4 N.D. N.D.
phage T7_phage 0.00015625 MOI TC4 N.D. -0.4 -1.5 N.D. N.D.
phage T7_phage 0.0015625 MOI TC1 N.D. -0.2 -1.6 N.D. N.D.
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. -1.4 -0.4 N.D. N.D.
nitrogen source Putrescine (N) N.D. -0.8 -1.0 N.D. N.D.
control PBS_cage_1_mice _Feces_16 hr N.D. -1.4 -0.3 N.D. N.D.
phage T7_phage 0.00015625 MOI TC2 N.D. -0.0 -1.7 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.3 -1.9 N.D. N.D.
phage Δdbn_10 N.D. -1.4 -0.1 N.D. N.D.
phage Ste41 N.D. -1.4 -0.0 N.D. N.D.
carbon source Glycolic Acid (C) N.D. -1.1 -0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM N.D. -1.6 +0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM N.D. -1.5 +0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM N.D. -1.5 +0.3 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.1 -1.2 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.2 -1.2 N.D. N.D.
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM N.D. -1.2 +0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM N.D. -1.2 +0.5 N.D. N.D.
carbon source carbon source D-Glucose 5mM N.D. -1.0 +0.4 N.D. N.D.
stress Cephalothin 0.01 mg/ml N.D. -1.2 +0.7 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.8 -1.2 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. +1.0 -1.1 N.D. N.D.
stress Cisplatin 0.1 mg/ml N.D. +1.0 -0.7 N.D. N.D.
control NoPredeControl N.D. -0.6 +0.9 N.D. N.D.
carbon source Adenosine 0.3742 mM (C) N.D. -0.5 +0.8 N.D. N.D.
carbon source Acetate (C) N.D. -0.4 +1.1 N.D. N.D.
carbon source Ying_sugar7 rep A; time point 3 N.D. -0.2 +1.1 N.D. N.D.
carbon source L-Malic (C) N.D. +0.5 +0.8 N.D. N.D.
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