Fitness for 5 genes in Escherichia coli BW25113

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500 ntybeD and dacA are separated by 109 nucleotidesdacA and rlpA are separated by 138 nucleotidesrlpA and mrdB are separated by 10 nucleotidesmrdB and mrdA are separated by 2 nucleotides b0631: ybeD - hypothetical protein (NCBI), at 661,602 to 661,865 ybeD b0632: dacA - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI), at 661,975 to 663,186 dacA b0633: rlpA - minor lipoprotein (NCBI), at 663,325 to 664,413 rlpA b0634: mrdB - cell wall shape-determining protein (NCBI), at 664,424 to 665,536 mrdB b0635: mrdA - transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2) (NCBI), at 665,539 to 667,440 mrdA
Group Condition b0631 b0632 b0633 b0634 b0635
ybeD dacA rlpA mrdB mrdA
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -4.8 -4.7 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -4.6 -4.4 N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 N.D. -4.9 -1.6 N.D. N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. -3.8 -2.6 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -2.9 -2.5 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -1.9 -2.3 N.D. N.D.
micoeukaryotes Acanthamoeba sp., muxed culture-2 N.D. -3.2 -1.0 N.D. N.D.
stress M9 with Methotrexate 1.98 mM 48 hr N.D. -3.9 -0.2 N.D. N.D.
stress Cephalothin 0.01 mg/ml N.D. -2.4 -1.5 N.D. N.D.
stress M9 with Methotrexate 1.98 mM 24 hr N.D. -3.7 -0.2 N.D. N.D.
micoeukaryotes Acanthamoeba sp., muxed culture-1 N.D. -3.2 -0.6 N.D. N.D.
stress M9 with Methotrexate 1.98 mM 48 hr N.D. -3.6 -0.2 N.D. N.D.
stress Trimethoprim 0.00125 mM N.D. -3.6 -0.2 N.D. N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr N.D. -3.5 -0.2 N.D. N.D.
stress M9 with Methotrexate 0.1 mM 48 hr N.D. -3.5 -0.2 N.D. N.D.
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr N.D. -3.4 -0.2 N.D. N.D.
no stress control anaerobic M9 no stress control; Anaerobic 48 hr N.D. -3.2 -0.2 N.D. N.D.
micoeukaryotes Dictyostellum sp.mixed culture-2 N.D. -2.5 -0.8 N.D. N.D.
no stress control anaerobic M9 no stress control; Anaerobic 24 hr N.D. -3.1 -0.2 N.D. N.D.
stress M9 with Methotrexate 1.98 mM 24 hr N.D. -3.2 -0.1 N.D. N.D.
carbon source Ying_all64 rep A; time point 3 N.D. -3.1 -0.1 N.D. N.D.
micoeukaryotes Dictyostellum sp.mixed culture-1 N.D. -2.4 -0.9 N.D. N.D.
carbon source Ying_all64 rep C; time point 3 N.D. -2.9 -0.2 N.D. N.D.
no stress control anaerobic M9 no stress control; Anaerobic 24 hr N.D. -3.0 -0.1 N.D. N.D.
stress Bacitracin 1 mg/ml N.D. -3.1 +0.3 N.D. N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. +3.8 +0.0 N.D. N.D.
carbon source carbon source D-Glucose 20mM N.D. +3.3 +0.6 N.D. N.D.
carbon source carbon source D-Glucose 20mM N.D. +3.2 +0.7 N.D. N.D.
carbon source carbon source D-Mannose 5mM N.D. +3.1 +1.1 N.D. N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. +4.3 +0.1 N.D. N.D.
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