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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
citF and citE are separated by 10 nucleotides
citE and citD overlap by 4 nucleotides
citD and citC are separated by 14 nucleotides
citC and citA are separated by 378 nucleotides
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
b0619: citA - sensory histidine kinase in two-component regulatory system with citB (NCBI), at 651,458 to 653,116
citA
Group
Condition
b0615
b0616
b0617
b0618
b0619
citF
citE
citD
citC
citA
phage
Ste41
-1.6
-0.6
-2.2
-0.5
-0.5
stress
octanol 0.08 vol%
+0.3
+0.2
-5.1
-0.3
-0.2
carbon source
carbon source D-Mannose 5mM
-1.3
-0.5
-1.6
-0.1
-0.7
phage
Ste41
-0.1
-0.2
-2.3
-0.9
+0.5
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.2
-0.5
-1.7
-0.2
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.5
-1.8
-0.2
+0.0
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.6
-1.2
+0.0
-0.5
phage
phage
-0.6
-0.5
-1.5
-0.3
+0.9
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.9
+0.2
-1.1
-0.3
+0.3
supernatant
LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
-0.1
-0.3
-1.4
-0.2
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.7
-0.6
-0.7
-0.7
+1.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.1
-0.2
-1.7
-0.3
+0.7
control
PBS_cage_1_mice _Feces_22 hr
+0.2
-0.4
-1.2
-0.3
+0.2
phage
RB68
-0.1
+0.0
+0.6
-0.3
-1.4
stress
LB Aerobic with Trimethoprim 0.00000125 mM
+0.5
+0.0
-1.9
+0.2
+0.1
motility
inner cut, LB soft agar motility assay
+0.2
-0.8
-1.4
+0.6
+0.4
stress
Bacitracin 1 mg/ml
-0.2
+0.3
-1.6
-0.0
+0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
+0.3
-1.1
+0.2
+0.4
phage
Δdbn_10
+0.5
+0.3
-0.6
+0.3
-1.0
no stress control anaerobic
M9 no stress control; Anaerobic 48 hr
-0.1
+0.2
+0.7
-0.0
-1.2
phage
T7_phage 0.0015625 MOI TC2
-1.0
+0.2
+0.8
-0.4
+0.0
carbon source
D-Galacturonic Acid (C) M9
+0.7
+0.2
-1.0
+0.2
-0.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.1
+0.2
-0.7
-0.5
+0.8
control
PBS_cage_2_mouse_3_Cecum_40 hr
+0.9
-0.1
-1.1
+0.4
+0.0
motility
inner cut, LB soft agar motility assay
-0.2
-0.6
+1.4
-0.2
+0.0
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.3
+0.1
-0.9
+0.9
+0.7
stress
Cisplatin 0.1 mg/ml
-0.5
+0.3
+0.7
+0.2
+0.7
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.3
+0.2
+1.9
-0.0
-0.3
stress
Nickel (II) chloride 1.5 mM
-0.1
+1.1
+0.8
+0.2
+0.2
stress
Chlorite 1.25 mM
+0.1
+0.6
+1.2
+0.5
+0.1
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