Fitness for 5 genes in Escherichia coli BW25113

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500 ntcitF and citE are separated by 10 nucleotidescitE and citD overlap by 4 nucleotidescitD and citC are separated by 14 nucleotidescitC and citA are separated by 378 nucleotides b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794 citF b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713 citE b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006 citD b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079 citC b0619: citA - sensory histidine kinase in two-component regulatory system with citB (NCBI), at 651,458 to 653,116 citA
Group Condition b0615 b0616 b0617 b0618 b0619
citF citE citD citC citA
phage Ste41 -1.6 -0.6 -2.2 -0.5 -0.5
stress octanol 0.08 vol% +0.3 +0.2 -5.1 -0.3 -0.2
carbon source carbon source D-Mannose 5mM -1.3 -0.5 -1.6 -0.1 -0.7
phage Ste41 -0.1 -0.2 -2.3 -0.9 +0.5
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 -0.5 -1.7 -0.2 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.5 -1.8 -0.2 +0.0
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.6 -1.2 +0.0 -0.5
phage phage -0.6 -0.5 -1.5 -0.3 +0.9
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.9 +0.2 -1.1 -0.3 +0.3
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.1 -0.3 -1.4 -0.2 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.7 -0.6 -0.7 -0.7 +1.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 -0.2 -1.7 -0.3 +0.7
control PBS_cage_1_mice _Feces_22 hr +0.2 -0.4 -1.2 -0.3 +0.2
phage RB68 -0.1 +0.0 +0.6 -0.3 -1.4
stress LB Aerobic with Trimethoprim 0.00000125 mM +0.5 +0.0 -1.9 +0.2 +0.1
motility inner cut, LB soft agar motility assay +0.2 -0.8 -1.4 +0.6 +0.4
stress Bacitracin 1 mg/ml -0.2 +0.3 -1.6 -0.0 +0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.3 -1.1 +0.2 +0.4
phage Δdbn_10 +0.5 +0.3 -0.6 +0.3 -1.0
no stress control anaerobic M9 no stress control; Anaerobic 48 hr -0.1 +0.2 +0.7 -0.0 -1.2
phage T7_phage 0.0015625 MOI TC2 -1.0 +0.2 +0.8 -0.4 +0.0
carbon source D-Galacturonic Acid (C) M9 +0.7 +0.2 -1.0 +0.2 -0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.1 +0.2 -0.7 -0.5 +0.8
control PBS_cage_2_mouse_3_Cecum_40 hr +0.9 -0.1 -1.1 +0.4 +0.0
motility inner cut, LB soft agar motility assay -0.2 -0.6 +1.4 -0.2 +0.0
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.3 +0.1 -0.9 +0.9 +0.7
stress Cisplatin 0.1 mg/ml -0.5 +0.3 +0.7 +0.2 +0.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.3 +0.2 +1.9 -0.0 -0.3
stress Nickel (II) chloride 1.5 mM -0.1 +1.1 +0.8 +0.2 +0.2
stress Chlorite 1.25 mM +0.1 +0.6 +1.2 +0.5 +0.1
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