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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
citG and citX overlap by 26 nucleotides
citX and citF are separated by 3 nucleotides
citF and citE are separated by 10 nucleotides
citE and citD overlap by 4 nucleotides
b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732
citG
b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258
citX
b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794
citF
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
Group
Condition
b0613
b0614
b0615
b0616
b0617
citG
citX
citF
citE
citD
stress
octanol 0.08 vol%
-0.1
N.D.
+0.3
+0.2
-5.1
phage
Ste41
+0.4
N.D.
-1.6
-0.6
-2.2
carbon source
carbon source D-Mannose 5mM
-0.0
N.D.
-1.3
-0.5
-1.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
N.D.
-0.7
-0.6
-0.7
phage
phage
-0.2
N.D.
-0.6
-0.5
-1.5
phage
Ste41
+0.0
N.D.
-0.1
-0.2
-2.3
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
-0.6
N.D.
-0.1
+0.0
-1.7
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.1
N.D.
-0.2
-0.5
-1.7
phage
Phage_N4_cage_4_mice _Feces_16 hr
-0.9
N.D.
-0.0
+0.3
-1.5
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
-0.4
N.D.
+0.3
-0.5
-1.0
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.5
N.D.
-0.1
-0.2
-1.7
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
-0.6
N.D.
+0.2
+0.2
-1.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
N.D.
+0.2
-0.6
-1.2
stress
LB Aerobic with Sulfamonomethoxine 0.00025 mM
+0.4
N.D.
-0.4
-0.2
-1.2
motility
inner cut, LB soft agar motility assay
+0.7
N.D.
+0.2
-0.8
-1.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.8
N.D.
+0.2
-0.5
-1.8
stress
LB Aerobic with Trimethoprim 0.00000125 mM
+0.3
N.D.
+0.5
+0.0
-1.9
phage
T7_phage 0.0015625 MOI TC2
-1.0
N.D.
-1.0
+0.2
+0.8
phage
Δdbn_10
-0.9
N.D.
+0.5
+0.3
-0.6
control
PBS_cage_2_mouse_3_Cecum_40 hr
-0.2
N.D.
+0.9
-0.1
-1.1
stress
Bacitracin 1 mg/ml
+1.0
N.D.
-0.2
+0.3
-1.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.8
N.D.
-0.3
+0.3
-1.1
motility
inner cut, LB soft agar motility assay
-0.6
N.D.
-0.2
-0.6
+1.4
stress
acetate 70 mM
+0.6
N.D.
+0.4
+0.2
-1.1
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.8
N.D.
-0.0
-0.1
+1.3
stress
Doxycycline hyclate 0.001 mg/ml
-0.7
N.D.
-0.2
+0.3
+1.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+2.4
N.D.
-0.9
+0.2
-1.1
stress
Chlorite 1.25 mM
-0.3
N.D.
+0.1
+0.6
+1.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+1.2
N.D.
+0.1
-0.2
+1.2
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.4
N.D.
+0.3
+0.2
+1.9
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