Fitness for 5 genes in Escherichia coli BW25113

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500 ntcitG and citX overlap by 26 nucleotidescitX and citF are separated by 3 nucleotidescitF and citE are separated by 10 nucleotidescitE and citD overlap by 4 nucleotides b0613: citG - triphosphoribosyl-dephospho-CoA transferase (NCBI), at 645,854 to 646,732 citG b0614: citX - 2'-(5'-triphosphoribosyl)-3'-dephospho-CoA:apo- citrate lyase (NCBI), at 646,707 to 647,258 citX b0615: citF - citrate lyase, citrate-ACP transferase (alpha) subunit (NCBI), at 647,262 to 648,794 citF b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713 citE b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006 citD
Group Condition b0613 b0614 b0615 b0616 b0617
citG citX citF citE citD
stress octanol 0.08 vol% -0.1 N.D. +0.3 +0.2 -5.1
phage Ste41 +0.4 N.D. -1.6 -0.6 -2.2
carbon source carbon source D-Mannose 5mM -0.0 N.D. -1.3 -0.5 -1.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.9 N.D. -0.7 -0.6 -0.7
phage phage -0.2 N.D. -0.6 -0.5 -1.5
phage Ste41 +0.0 N.D. -0.1 -0.2 -2.3
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -0.6 N.D. -0.1 +0.0 -1.7
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.1 N.D. -0.2 -0.5 -1.7
phage Phage_N4_cage_4_mice _Feces_16 hr -0.9 N.D. -0.0 +0.3 -1.5
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM -0.4 N.D. +0.3 -0.5 -1.0
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.5 N.D. -0.1 -0.2 -1.7
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM -0.6 N.D. +0.2 +0.2 -1.3
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 N.D. +0.2 -0.6 -1.2
stress LB Aerobic with Sulfamonomethoxine 0.00025 mM +0.4 N.D. -0.4 -0.2 -1.2
motility inner cut, LB soft agar motility assay +0.7 N.D. +0.2 -0.8 -1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 N.D. +0.2 -0.5 -1.8
stress LB Aerobic with Trimethoprim 0.00000125 mM +0.3 N.D. +0.5 +0.0 -1.9
phage T7_phage 0.0015625 MOI TC2 -1.0 N.D. -1.0 +0.2 +0.8
phage Δdbn_10 -0.9 N.D. +0.5 +0.3 -0.6
control PBS_cage_2_mouse_3_Cecum_40 hr -0.2 N.D. +0.9 -0.1 -1.1
stress Bacitracin 1 mg/ml +1.0 N.D. -0.2 +0.3 -1.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 N.D. -0.3 +0.3 -1.1
motility inner cut, LB soft agar motility assay -0.6 N.D. -0.2 -0.6 +1.4
stress acetate 70 mM +0.6 N.D. +0.4 +0.2 -1.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.8 N.D. -0.0 -0.1 +1.3
stress Doxycycline hyclate 0.001 mg/ml -0.7 N.D. -0.2 +0.3 +1.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +2.4 N.D. -0.9 +0.2 -1.1
stress Chlorite 1.25 mM -0.3 N.D. +0.1 +0.6 +1.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.2 N.D. +0.1 -0.2 +1.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.4 N.D. +0.3 +0.2 +1.9
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