Fitness for 5 genes in Escherichia coli BW25113

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500 ntentA and ybdB are separated by 2 nucleotidesybdB and cstA are separated by 180 nucleotidescstA and ybdD are separated by 182 nucleotidesybdD and ybdH are separated by 9 nucleotides b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520 entA b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936 ybdB b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222 cstA b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602 ybdD b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700 ybdH
Group Condition b0596 b0597 b0598 b4512 b0599
entA ybdB cstA ybdD ybdH
control PBS_cage_1_mouse_1_Cecum_40 hr +0.1 -1.8 -0.2 N.D. -0.4
control PBS_cage_1_mice _Feces_22 hr -0.4 -1.4 +0.2 N.D. -0.3
phage T7_phage 0.0015625 MOI TC2 -0.8 -0.8 +0.1 N.D. -0.4
phage T7_phage 0.00015625 MOI TC4 -0.6 +0.4 -0.3 N.D. -0.9
phage T7_phage 0.0015625 MOI TC1 -0.5 +0.1 -0.4 N.D. -0.5
stress Cephalothin 0.01 mg/ml -0.5 -0.6 -0.3 N.D. +0.3
carbon source carbon source D-Glucose 0.25mM -0.7 -0.7 +0.1 N.D. +0.2
carbon source D-Serine (C) +0.4 -0.6 -1.0 N.D. +0.2
carbon source Ying_Nucleobase14 rep A; time point 2 -0.7 -0.3 +0.1 N.D. +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 -0.4 -0.7 N.D. -0.1
stress Cisplatin 0.1 mg/ml -1.0 +0.7 -0.1 N.D. -0.2
motility outer cut, LB soft agar motility assay -0.7 +0.2 +0.3 N.D. -0.2
carbon source L-Malic (C) +0.5 +0.1 -1.4 N.D. +0.3
carbon source D-Serine (C) +0.3 +0.1 -0.9 N.D. +0.1
carbon source L-Malic (C) +0.8 +0.2 -1.4 N.D. +0.3
carbon source carbon source D-Glucose 5mM +0.9 -1.2 -0.1 N.D. +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 +0.1 -0.6 N.D. +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.9 -0.1 -0.4 N.D. +0.0
control NoBALOControl +0.7 +0.3 +0.1 N.D. -0.4
carbon source succinate (C) +0.7 -0.4 -0.0 N.D. +0.3
phage Ste41 -0.2 -0.1 +0.6 N.D. +0.6
nophagecontrol R1_N4_T1_phage0 +0.5 -0.2 +0.4 N.D. +0.3
carbon source carbon source D-Mannose 20mM +0.8 +0.4 -0.1 N.D. +0.2
carbon source a-Ketoglutaric (C) +0.8 +0.3 -0.0 N.D. +0.3
nitrogen source L-Arginine (N) +1.2 +0.1 -0.1 N.D. +0.1
carbon source a-Ketoglutaric (C) +0.7 +0.4 +0.1 N.D. +0.3
phage N4_phage 0.00009375 MOI TC4 +0.6 +0.7 +0.1 N.D. +0.1
nitrogen source L-Arginine (N) +1.1 +0.4 +0.1 N.D. +0.0
carbon source acetate (C) +0.8 +0.4 +0.1 N.D. +0.4
carbon source carbon source D-Mannose 0.5mM +0.4 +0.9 +0.1 N.D. +0.5
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