Fitness for 5 genes in Escherichia coli BW25113

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500 ntentF and fepE are separated by 215 nucleotidesfepE and fepC overlap by 4 nucleotidesfepC and fepG overlap by 4 nucleotidesfepG and fepD overlap by 4 nucleotides b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261 entF b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610 fepE b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422 fepC b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411 fepG b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412 fepD
Group Condition b0586 b0587 b0588 b0589 b0590
entF fepE fepC fepG fepD
carbon source Ying_Nucleobase14 rep A; time point 3 -0.6 +0.1 -5.7 -6.5 -6.2
carbon source Ying_Nucleobase14 rep C; time point 3 -0.7 +0.0 -5.9 -6.0 -6.0
carbon source Ying_Nucleobase14 rep B; time point 3 -0.8 -0.0 -6.8 -4.8 -6.1
carbon source Ying_AminoAcid20 rep C; time point 3 -0.1 -0.0 -4.8 -4.8 -5.2
msc 22hr_mediaonly_rep2_lowinoculum +0.1 +0.0 -4.4 -5.1 -5.0
carbon source Ying_AminoAcid20 rep A; time point 3 -0.3 -0.2 -4.8 -4.5 -4.6
msc 22hr_mediaonly_rep1_lowinoculum +0.2 -0.1 -4.0 -4.9 -4.3
carbon source Ying_AminoAcid20 rep B; time point 3 -0.2 -0.0 -3.8 -3.6 -4.7
carbon source D-Galacturonic Acid (C) M9 -0.2 -0.0 -4.3 -4.0 -3.6
msc 22hr_MSC_rep2_lowinoculum +0.4 +0.4 -3.5 -4.6 -4.7
M9 Glucose 2% aerobic -0.6 -0.4 -3.5 -3.7 -3.3
carbon source Glycolic Acid (C) -0.0 -0.2 -3.7 -3.8 -3.8
M9 Glucose 2% aerobic -0.5 -0.5 -3.3 -3.3 -3.5
carbon source L-Malic (C) +0.0 +0.0 -3.3 -3.5 -3.6
msc 22hr_MSC_rep1_lowinoculum +0.3 -0.2 -3.3 -3.7 -3.4
carbon source acetate (C) -0.1 +0.1 -3.6 -3.1 -3.6
protonated/deuterated deuterated glucose in D2O -0.0 +0.0 -3.4 -3.5 -3.4
msc 6hr_mediaonly_rep2_lowinoculum -0.1 +0.1 -2.8 -3.5 -3.5
protonated/deuterated deuterated glucose in D2O -0.1 +0.1 -3.8 -3.1 -3.0
carbon source Ying_Nucleobase14 rep B; time point 2 -0.5 +0.1 -3.4 -2.6 -3.5
protonated/deuterated deuterated glucose in D2O -0.2 +0.1 -3.4 -3.0 -3.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 -0.1 +3.0 +3.0 +3.5
plasmid transformation mariner vector transformation; agar assay +0.2 -0.0 +3.4 +3.2 +3.4
plasmid transformation vcDART vector transformation; agar assay +0.4 -0.3 +3.4 +3.5 +3.5
plasmid transformation vcDART vector transformation; agar assay +0.4 +0.1 +3.4 +3.4 +3.7
plasmid transformation vcDART vector transformation; agar assay +0.4 -0.0 +3.5 +3.5 +3.6
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 -0.3 +3.5 +3.6 +4.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.5 +0.1 +3.7 +3.4 +3.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 +0.1 +3.9 +3.9 +4.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.7 -0.1 +4.3 +4.0 +4.6
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