Fitness for 5 genes in Escherichia coli BW25113

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500 ntinsL-2 and entD are separated by 281 nucleotidesentD and fepA are separated by 174 nucleotidesfepA and fes are separated by 320 nucleotidesfes and ybdZ are separated by 2 nucleotides b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400 insL-2 b0583: entD - phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI), at 608,682 to 609,302 entD b0584: fepA - iron-enterobactin outer membrane transporter (NCBI), at 609,477 to 611,717 fepA b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162 fes b4511: ybdZ - hypothetical protein (NCBI), at 613,165 to 613,383 ybdZ
Group Condition b0582 b0583 b0584 b0585 b4511
insL-2 entD fepA fes ybdZ
msc 22hr_mediaonly_rep1_lowinoculum N.D. -0.7 -4.6 -4.3 +0.1
msc 22hr_mediaonly_rep2_lowinoculum N.D. -0.2 -4.8 -4.0 -0.1
msc 22hr_MSC_rep1_lowinoculum N.D. +0.0 -3.3 -4.6 +0.3
msc 22hr_MSC_rep2_lowinoculum N.D. +0.2 -3.3 -3.9 +0.2
carbon source Ying_Nucleobase14 rep C; time point 3 N.D. -0.8 -0.2 -5.3 -0.2
protonated/deuterated protonated glucose in D2O N.D. -0.5 -0.6 -4.3 -0.8
carbon source a-Ketoglutaric (C) N.D. -0.4 -2.6 -3.5 +0.3
protonated/deuterated deuterated glucose in D2O N.D. -0.4 -1.0 -4.2 -0.4
carbon source a-Ketoglutaric (C) N.D. -0.5 -3.2 -2.8 +0.4
carbon source acetate (C) N.D. -0.5 -1.5 -4.0 +0.2
carbon source L-Malic (C) N.D. -0.1 -1.3 -4.1 -0.2
msc 6hr_mediaonly_rep2_lowinoculum N.D. -0.1 -2.8 -2.6 -0.3
protonated/deuterated deuterated glucose in D2O N.D. -0.2 -0.8 -5.0 +0.2
protonated/deuterated deuterated glucose in D2O N.D. -0.8 -1.0 -4.2 +0.3
protonated/deuterated protonated glucose in D2O N.D. -0.4 -0.7 -4.3 -0.2
carbon source L-Malic (C) N.D. -0.5 -1.5 -4.0 +0.5
carbon source Ying_Nucleobase14 rep A; time point 3 N.D. -0.8 -0.1 -4.0 -0.4
msc 6hr_MSC_rep1_lowinoculum N.D. -0.0 -2.2 -3.2 +0.1
carbon source Ying_Nucleobase14 rep B; time point 3 N.D. -0.7 -0.1 -4.5 +0.3
msc 6hr_MSC_rep2_lowinoculum N.D. +0.1 -2.4 -2.9 +0.3
phage TP4 N.D. -0.3 -4.5 +0.1 -0.2
protonated/deuterated protonated glucose in D2O N.D. -0.3 -0.4 -4.1 +0.0
carbon source Glycolic Acid (C) N.D. -0.4 -0.3 -4.6 +0.6
carbon source Ying_sugar7 rep C; time point 3 N.D. -0.5 -0.2 -4.1 +0.2
carbon source Ying_AminoAcid20 rep B; time point 3 N.D. -0.3 -0.2 -4.2 +0.2
carbon source acetate (C) N.D. -0.3 -1.4 -3.2 +0.3
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr N.D. -0.3 -1.7 -2.6 +0.1
carbon source succinate (C) N.D. -0.4 -1.0 -3.2 +0.3
msc 22hr_MSC_rep1_highinoculum N.D. +0.5 -1.5 -2.8 -0.2
carbon source Glycolic Acid (C) N.D. -0.2 -0.2 -3.8 +0.8
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