Fitness for 5 genes in Escherichia coli BW25113

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500 ntybdG and nfnB are separated by 107 nucleotidesnfnB and ybdF are separated by 93 nucleotidesybdF and ybdJ are separated by 64 nucleotidesybdJ and ybdK are separated by 65 nucleotides b0577: ybdG - predicted mechanosensitive channel (NCBI), at 602,639 to 603,886 ybdG b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647 nfnB b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109 ybdF b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422 ybdJ b0581: ybdK - gamma-glutamyl:cysteine ligase (NCBI), at 605,488 to 606,606 ybdK
Group Condition b0577 b0578 b0579 b0580 b0581
ybdG nfnB ybdF ybdJ ybdK
control PBS_cage_1_mouse_1_Cecum_40 hr -0.0 -0.0 -2.2 -1.6 +0.1
carbon source carbon source D-Mannose 0.5mM -0.1 +0.1 -0.1 -2.8 -0.1
carbon source carbon source D-Mannose 1mM +0.0 -0.0 -1.1 -1.0 -0.7
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 -0.7 -0.4 -1.5 -0.2
stress Cisplatin 0.05 mg/ml -0.8 -0.4 -0.3 -0.7 -0.2
phage Papa dilution 10-1 cacl2 maltose -0.4 -0.7 -0.9 +0.1 -0.3
phage Lambda_ci1857 -0.5 +0.1 -0.2 -1.4 -0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.0 +0.6 -0.6 -1.7 +0.0
stress 3-methyl-1-butanol 1.25 vol% -0.2 -0.6 +1.0 -1.3 -0.2
control PBS_cage_1_mouse_2_Cecum_40 hr -0.1 -0.3 +0.5 -1.2 -0.3
control PBS_cage_2_mouse_2_Cecum_40 hr +0.3 -0.2 -0.4 -1.2 +0.2
stress Cisplatin 0.1 mg/ml -0.3 +0.6 -1.2 -0.7 +0.4
stress LB Aerobic with Oxytetracycline 0.00000125 mM -0.1 +0.4 +0.4 -1.3 -0.1
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 +0.5 -0.0 -1.5 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.0 +0.4 -1.7 +0.4 +0.1
stress Cisplatin 0.1 mg/ml -1.1 -0.4 +0.0 +0.9 -0.1
phage Ste41 -0.4 -0.4 -1.4 +1.1 +0.7
motility outer cut, LB soft agar motility assay +0.2 -0.4 +0.9 -0.5 +0.2
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.2 +2.2 -1.0 -1.3 +0.3
stress 2-nitrobenzenesulfonyl fluoride -0.1 +2.7 -1.2 -1.2 +0.3
carbon source carbon source D-Glucose 0.25mM -0.1 +0.4 +1.0 -0.7 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.0 +1.9 -0.4 -0.6 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.4 -0.5 +1.4 +0.0
motility inner cut, LB soft agar motility assay -0.0 +0.3 -0.8 +1.4 +0.6
carbon source Dodecanol and Rhamnolipid (C) -0.0 +0.3 +0.4 +1.2 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -0.4 -0.2 +2.0 +0.3
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.1 +0.5 -0.4 +1.3 +0.3
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.3 +5.9 -2.9 -1.3 +1.1
carbon source carbon source D-Mannose 0.5mM +0.1 +0.3 +0.9 +1.4 -0.0
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.2 +2.5 -0.7 +0.0 +1.1
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