Fitness for 5 genes in Escherichia coli BW25113

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500 ntpheP and ybdG are separated by 80 nucleotidesybdG and nfnB are separated by 107 nucleotidesnfnB and ybdF are separated by 93 nucleotidesybdF and ybdJ are separated by 64 nucleotides b0576: pheP - phenylalanine transporter (NCBI), at 601,182 to 602,558 pheP b0577: ybdG - predicted mechanosensitive channel (NCBI), at 602,639 to 603,886 ybdG b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647 nfnB b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109 ybdF b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422 ybdJ
Group Condition b0576 b0577 b0578 b0579 b0580
pheP ybdG nfnB ybdF ybdJ
control PBS_cage_1_mouse_1_Cecum_40 hr -0.2 -0.0 -0.0 -2.2 -1.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 +0.2 -0.7 -0.4 -1.5
carbon source carbon source D-Mannose 0.5mM +0.0 -0.1 +0.1 -0.1 -2.8
stress Cisplatin 0.05 mg/ml -0.1 -0.8 -0.4 -0.3 -0.7
phage Papa dilution 10-1 cacl2 maltose -0.4 -0.4 -0.7 -0.9 +0.1
carbon source carbon source D-Mannose 1mM -0.2 +0.0 -0.0 -1.1 -1.0
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.3 +0.0 +0.6 -0.6 -1.7
phage Lambda_ci1857 +0.2 -0.5 +0.1 -0.2 -1.4
stress Cisplatin 0.1 mg/ml -0.1 -0.3 +0.6 -1.2 -0.7
phage Ste41 -0.5 -0.4 -0.4 -1.4 +1.1
control PBS_cage_2_mouse_2_Cecum_40 hr +0.3 +0.3 -0.2 -0.4 -1.2
phage T7_phage 0.0015625 MOI TC2 -0.3 +0.2 +0.5 +0.0 -1.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.1 +0.0 +0.4 -1.7 +0.4
stress Cisplatin 0.1 mg/ml -0.3 -1.1 -0.4 +0.0 +0.9
stress 3-methyl-1-butanol 1.25 vol% +0.3 -0.2 -0.6 +1.0 -1.3
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.2 +0.5 -0.0 -1.5
stress LB Aerobic with Oxytetracycline 0.00000125 mM +0.2 -0.1 +0.4 +0.4 -1.3
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.2 +0.2 +2.2 -1.0 -1.3
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.6 -0.0 +1.9 -0.4 -0.6
carbon source carbon source D-Glucose 0.25mM -0.2 -0.1 +0.4 +1.0 -0.7
stress 2-nitrobenzenesulfonyl fluoride +0.3 -0.1 +2.7 -1.2 -1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -0.1 -0.4 -0.2 +2.0
motility inner cut, LB soft agar motility assay -0.0 -0.0 +0.3 -0.8 +1.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.2 +0.4 -0.5 +1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 -0.1 +0.2 -0.3 +1.1
stress LB Anaerobic with Ampicillin 0.00000195313 mM -0.4 +0.0 +0.1 +0.0 +1.8
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.0 +0.1 +0.5 -0.4 +1.3
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.3 -0.3 +5.9 -2.9 -1.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -0.2 +2.5 -0.7 +0.0
carbon source carbon source D-Mannose 0.5mM -0.2 +0.1 +0.3 +0.9 +1.4
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