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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
pheP and ybdG are separated by 80 nucleotides
ybdG and nfnB are separated by 107 nucleotides
nfnB and ybdF are separated by 93 nucleotides
ybdF and ybdJ are separated by 64 nucleotides
b0576: pheP - phenylalanine transporter (NCBI), at 601,182 to 602,558
pheP
b0577: ybdG - predicted mechanosensitive channel (NCBI), at 602,639 to 603,886
ybdG
b0578: nfnB - dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI), at 603,994 to 604,647
nfnB
b0579: ybdF - hypothetical protein (NCBI), at 604,741 to 605,109
ybdF
b0580: ybdJ - predicted inner membrane protein (NCBI), at 605,174 to 605,422
ybdJ
Group
Condition
b0576
b0577
b0578
b0579
b0580
pheP
ybdG
nfnB
ybdF
ybdJ
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.2
-0.0
-0.0
-2.2
-1.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
+0.2
-0.7
-0.4
-1.5
carbon source
carbon source D-Mannose 0.5mM
+0.0
-0.1
+0.1
-0.1
-2.8
stress
Cisplatin 0.05 mg/ml
-0.1
-0.8
-0.4
-0.3
-0.7
phage
Papa dilution 10-1 cacl2 maltose
-0.4
-0.4
-0.7
-0.9
+0.1
carbon source
carbon source D-Mannose 1mM
-0.2
+0.0
-0.0
-1.1
-1.0
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.3
+0.0
+0.6
-0.6
-1.7
phage
Lambda_ci1857
+0.2
-0.5
+0.1
-0.2
-1.4
stress
Cisplatin 0.1 mg/ml
-0.1
-0.3
+0.6
-1.2
-0.7
phage
Ste41
-0.5
-0.4
-0.4
-1.4
+1.1
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.3
+0.3
-0.2
-0.4
-1.2
phage
T7_phage 0.0015625 MOI TC2
-0.3
+0.2
+0.5
+0.0
-1.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.1
+0.0
+0.4
-1.7
+0.4
stress
Cisplatin 0.1 mg/ml
-0.3
-1.1
-0.4
+0.0
+0.9
stress
3-methyl-1-butanol 1.25 vol%
+0.3
-0.2
-0.6
+1.0
-1.3
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.3
+0.2
+0.5
-0.0
-1.5
stress
LB Aerobic with Oxytetracycline 0.00000125 mM
+0.2
-0.1
+0.4
+0.4
-1.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.2
+0.2
+2.2
-1.0
-1.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.6
-0.0
+1.9
-0.4
-0.6
carbon source
carbon source D-Glucose 0.25mM
-0.2
-0.1
+0.4
+1.0
-0.7
stress
2-nitrobenzenesulfonyl fluoride
+0.3
-0.1
+2.7
-1.2
-1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.5
-0.1
-0.4
-0.2
+2.0
motility
inner cut, LB soft agar motility assay
-0.0
-0.0
+0.3
-0.8
+1.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
-0.2
+0.4
-0.5
+1.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
-0.1
+0.2
-0.3
+1.1
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-0.4
+0.0
+0.1
+0.0
+1.8
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.0
+0.1
+0.5
-0.4
+1.3
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.3
-0.3
+5.9
-2.9
-1.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.2
-0.2
+2.5
-0.7
+0.0
carbon source
carbon source D-Mannose 0.5mM
-0.2
+0.1
+0.3
+0.9
+1.4
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