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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
nmpC and insH-2 are separated by 150 nucleotides
insH-2 and essD are separated by 1644 nucleotides
essD and ybcS overlap by 1 nucleotides
ybcS and rzpD overlap by 4 nucleotides
b0553: nmpC - DLP12 prophage; truncated outer membrane porin (pseudogene) (RefSeq), at 573,752 to 573,809
nmpC
b0552: insH-2 - DLP12 prophage; IS5 transposase and trans-activator (NCBI), at 573,960 to 574,976
insH-2
b0554: essD - DLP12 prophage; predicted phage lysis protein (NCBI), at 576,621 to 576,836
essD
b0555: ybcS - DLP12 prophage; predicted lysozyme (NCBI), at 576,836 to 577,333
ybcS
b0556: rzpD - DLP12 prophage; predicted murein endopeptidase (NCBI), at 577,330 to 577,791
rzpD
Group
Condition
b0553
b0552
b0554
b0555
b0556
nmpC
insH-2
essD
ybcS
rzpD
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.3
N.D.
-0.1
-0.4
-2.1
phage
Papa dilution 10-1 cacl2 maltose
-0.6
N.D.
-1.0
-0.8
-0.3
phage
Δdbn_10
-0.2
N.D.
-0.1
-1.9
-0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.1
N.D.
+0.2
-0.4
-0.8
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.1
N.D.
-0.7
-0.4
-0.6
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.2
N.D.
-0.3
-0.2
-1.0
drt_type_3
DRT_type_3
-0.4
N.D.
-0.8
-0.3
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
N.D.
-0.4
-0.1
-1.4
fr_1000_e3
Fr_1000_E3
-0.2
N.D.
-0.3
-0.5
-0.5
mzaabcde
mzaABCDE
-0.3
N.D.
-0.6
-0.1
-0.4
retron_ec_48_control
retron_Ec_48_control
-0.2
N.D.
-0.7
-0.4
+0.1
sir2-hera
SIR2-HerA
-0.5
N.D.
-0.5
-0.4
+0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.5
N.D.
-0.5
-0.2
+0.5
ppi
ppI
-0.3
N.D.
-0.5
-0.3
+0.5
gfp_control
GFP_control
-0.5
N.D.
-0.4
-0.3
+0.7
stress
Cisplatin 0.1 mg/ml
-0.3
N.D.
+0.3
+0.4
-0.8
sir2-hera
SIR2-HerA
-0.3
N.D.
-0.6
-0.2
+0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.7
N.D.
+0.8
-0.4
-0.1
stress
3-methyl-1-butanol 1.25 vol%
-0.0
N.D.
-0.5
-0.3
+0.7
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.2
N.D.
+0.3
+0.7
-0.9
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
-0.2
N.D.
-0.1
-0.0
+1.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.2
N.D.
-0.2
+0.1
+1.1
nophagecontrol
R1_N4_T1_phage0
-0.1
N.D.
-0.4
+0.5
+1.0
carbon source
Ying_Nucleobase14 rep B; time point 2
-0.2
N.D.
+0.2
+0.2
+1.1
control
PBS_cage_2_mouse_2_Cecum_40 hr
-0.1
N.D.
+0.1
+0.6
+0.8
phage
T7_phage 0.0015625 MOI TC1
+0.0
N.D.
+0.6
+0.7
+0.2
carbon source
Ying_Nucleobase14 rep C; time point 3
-0.1
N.D.
-0.1
+0.2
+1.6
carbon source
Ying_Nucleobase14 rep A; time point 3
-0.1
N.D.
+0.0
+0.1
+1.7
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.2
N.D.
+0.1
+0.1
+1.8
phage
R2_N4_T2_phage10
+0.2
N.D.
+0.5
+0.6
+0.8
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