Fitness for 5 genes in Escherichia coli BW25113

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500 ntnmpC and insH-2 are separated by 150 nucleotidesinsH-2 and essD are separated by 1644 nucleotidesessD and ybcS overlap by 1 nucleotidesybcS and rzpD overlap by 4 nucleotides b0553: nmpC - DLP12 prophage; truncated outer membrane porin (pseudogene) (RefSeq), at 573,752 to 573,809 nmpC b0552: insH-2 - DLP12 prophage; IS5 transposase and trans-activator (NCBI), at 573,960 to 574,976 insH-2 b0554: essD - DLP12 prophage; predicted phage lysis protein (NCBI), at 576,621 to 576,836 essD b0555: ybcS - DLP12 prophage; predicted lysozyme (NCBI), at 576,836 to 577,333 ybcS b0556: rzpD - DLP12 prophage; predicted murein endopeptidase (NCBI), at 577,330 to 577,791 rzpD
Group Condition b0553 b0552 b0554 b0555 b0556
nmpC insH-2 essD ybcS rzpD
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.3 N.D. -0.1 -0.4 -2.1
phage Papa dilution 10-1 cacl2 maltose -0.6 N.D. -1.0 -0.8 -0.3
phage Δdbn_10 -0.2 N.D. -0.1 -1.9 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -1.1 N.D. +0.2 -0.4 -0.8
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.1 N.D. -0.7 -0.4 -0.6
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.2 N.D. -0.3 -0.2 -1.0
drt_type_3 DRT_type_3 -0.4 N.D. -0.8 -0.3 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 N.D. -0.4 -0.1 -1.4
fr_1000_e3 Fr_1000_E3 -0.2 N.D. -0.3 -0.5 -0.5
mzaabcde mzaABCDE -0.3 N.D. -0.6 -0.1 -0.4
retron_ec_48_control retron_Ec_48_control -0.2 N.D. -0.7 -0.4 +0.1
sir2-hera SIR2-HerA -0.5 N.D. -0.5 -0.4 +0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 N.D. -0.5 -0.2 +0.5
ppi ppI -0.3 N.D. -0.5 -0.3 +0.5
gfp_control GFP_control -0.5 N.D. -0.4 -0.3 +0.7
stress Cisplatin 0.1 mg/ml -0.3 N.D. +0.3 +0.4 -0.8
sir2-hera SIR2-HerA -0.3 N.D. -0.6 -0.2 +0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.7 N.D. +0.8 -0.4 -0.1
stress 3-methyl-1-butanol 1.25 vol% -0.0 N.D. -0.5 -0.3 +0.7
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 N.D. +0.3 +0.7 -0.9
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.2 N.D. -0.1 -0.0 +1.1
stress LB Aerobic with Irgasan 0.0000003125 mM -0.2 N.D. -0.2 +0.1 +1.1
nophagecontrol R1_N4_T1_phage0 -0.1 N.D. -0.4 +0.5 +1.0
carbon source Ying_Nucleobase14 rep B; time point 2 -0.2 N.D. +0.2 +0.2 +1.1
control PBS_cage_2_mouse_2_Cecum_40 hr -0.1 N.D. +0.1 +0.6 +0.8
phage T7_phage 0.0015625 MOI TC1 +0.0 N.D. +0.6 +0.7 +0.2
carbon source Ying_Nucleobase14 rep C; time point 3 -0.1 N.D. -0.1 +0.2 +1.6
carbon source Ying_Nucleobase14 rep A; time point 3 -0.1 N.D. +0.0 +0.1 +1.7
carbon source Ying_Nucleobase14 rep B; time point 3 -0.2 N.D. +0.1 +0.1 +1.8
phage R2_N4_T2_phage10 +0.2 N.D. +0.5 +0.6 +0.8
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