Fitness for 5 genes in Escherichia coli BW25113

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500 ntybbM and ybbN are separated by 62 nucleotidesybbN and ybbO are separated by 60 nucleotidesybbO and tesA overlap by 11 nucleotidestesA and ybbA overlap by 33 nucleotides b0491: ybbM - putative metal resistance protein (VIMSS), at 515,807 to 516,586 ybbM b0492: ybbN - putative thioredoxin-like protein (VIMSS), at 516,649 to 517,503 ybbN b0493: ybbO - short chain dehydrogenase (NCBI), at 517,564 to 518,373 ybbO b0494: tesA - multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI), at 518,363 to 518,989 tesA b0495: ybbA - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 518,957 to 519,643 ybbA
Group Condition b0491 b0492 b0493 b0494 b0495
ybbM ybbN ybbO tesA ybbA
motility outer cut, LB soft agar motility assay +0.0 +0.1 -0.5 -1.1 -1.1
stress Benzalkonium Chloride 0.004 mg/ml -0.3 -0.1 +0.2 -0.9 -1.2
motility outer cut, LB soft agar motility assay -0.2 -0.2 +0.3 -0.2 -1.5
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.1 -0.1 -0.9 +0.3 -0.6
phage Ste41 -0.2 +0.2 -0.8 -0.7 +0.1
motility inner cut, LB soft agar motility assay +0.1 +0.2 -0.5 -0.6 -0.5
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.2 -0.4 -0.6 -0.6 +0.2
phage T7_phage 0.0015625 MOI TC2 +0.1 -0.3 +0.5 -0.1 -1.2
phage TP4 -0.7 +0.0 -0.2 +0.5 -0.6
stress 3-methyl-2-butenol 1.25 vol% +0.1 +0.2 +0.2 -0.5 -0.9
stress octanol 0.08 vol% +0.2 +0.5 +0.1 -0.4 -1.1
phage T7_phage 0.00015625 MOI TC4 +1.1 +0.1 -0.3 -0.5 -0.9
carbon source carbon source D-Mannose 1mM -0.9 +0.2 +0.1 -0.4 +0.4
carbon source carbon source D-Glucose 5mM -0.1 +0.5 -0.9 -0.3 +0.4
phage Lambda_ci1857 +0.1 +0.1 +0.7 -0.8 -0.3
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -0.3 +0.6 -0.6 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -0.8 +0.9 -0.1 -0.1
stress Cisplatin 0.1 mg/ml -0.4 -0.5 -0.3 +0.5 +0.3
phage T7_phage 0.0015625 MOI TC1 +0.9 +0.1 -0.0 -0.5 -0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 -0.7 +0.3 +0.3 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.1 +0.2 +1.2 -1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.2 +0.5 +0.5 +0.5
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.1 +0.5 +0.4 -0.7 +0.7
carbon source carbon source D-Mannose 5mM -0.4 +0.7 +0.4 +0.2 +0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 +0.3 +0.4 +0.8 +0.4
protonated/deuterated deuterated glucose in H2O +0.0 +0.5 +0.5 +0.7 +0.7
protonated/deuterated deuterated glucose in D2O +0.1 +0.7 +0.7 +0.6 +0.5
protonated/deuterated deuterated glucose in D2O +0.1 +0.8 +0.8 +0.6 +0.5
protonated/deuterated deuterated glucose in H2O +0.2 +0.6 +1.0 +0.9 +0.8
protonated/deuterated deuterated glucose in H2O +0.7 +0.6 +1.0 +1.2 +0.8
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