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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ybbM and ybbN are separated by 62 nucleotides
ybbN and ybbO are separated by 60 nucleotides
ybbO and tesA overlap by 11 nucleotides
tesA and ybbA overlap by 33 nucleotides
b0491: ybbM - putative metal resistance protein (VIMSS), at 515,807 to 516,586
ybbM
b0492: ybbN - putative thioredoxin-like protein (VIMSS), at 516,649 to 517,503
ybbN
b0493: ybbO - short chain dehydrogenase (NCBI), at 517,564 to 518,373
ybbO
b0494: tesA - multifunctional acyl-CoA thioesterase I and protease I and lysophospholipase L1 (NCBI), at 518,363 to 518,989
tesA
b0495: ybbA - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 518,957 to 519,643
ybbA
Group
Condition
b0491
b0492
b0493
b0494
b0495
ybbM
ybbN
ybbO
tesA
ybbA
motility
outer cut, LB soft agar motility assay
+0.0
+0.1
-0.5
-1.1
-1.1
stress
Benzalkonium Chloride 0.004 mg/ml
-0.3
-0.1
+0.2
-0.9
-1.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.2
+0.3
-0.2
-1.5
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.1
-0.1
-0.9
+0.3
-0.6
phage
Ste41
-0.2
+0.2
-0.8
-0.7
+0.1
motility
inner cut, LB soft agar motility assay
+0.1
+0.2
-0.5
-0.6
-0.5
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.2
-0.4
-0.6
-0.6
+0.2
phage
T7_phage 0.0015625 MOI TC2
+0.1
-0.3
+0.5
-0.1
-1.2
phage
TP4
-0.7
+0.0
-0.2
+0.5
-0.6
stress
3-methyl-2-butenol 1.25 vol%
+0.1
+0.2
+0.2
-0.5
-0.9
stress
octanol 0.08 vol%
+0.2
+0.5
+0.1
-0.4
-1.1
phage
T7_phage 0.00015625 MOI TC4
+1.1
+0.1
-0.3
-0.5
-0.9
carbon source
carbon source D-Mannose 1mM
-0.9
+0.2
+0.1
-0.4
+0.4
carbon source
carbon source D-Glucose 5mM
-0.1
+0.5
-0.9
-0.3
+0.4
phage
Lambda_ci1857
+0.1
+0.1
+0.7
-0.8
-0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.2
-0.3
+0.6
-0.6
+0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.2
-0.8
+0.9
-0.1
-0.1
stress
Cisplatin 0.1 mg/ml
-0.4
-0.5
-0.3
+0.5
+0.3
phage
T7_phage 0.0015625 MOI TC1
+0.9
+0.1
-0.0
-0.5
-0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
-0.7
+0.3
+0.3
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
+0.1
+0.2
+1.2
-1.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.2
+0.5
+0.5
+0.5
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.1
+0.5
+0.4
-0.7
+0.7
carbon source
carbon source D-Mannose 5mM
-0.4
+0.7
+0.4
+0.2
+0.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
+0.3
+0.4
+0.8
+0.4
protonated/deuterated
deuterated glucose in H2O
+0.0
+0.5
+0.5
+0.7
+0.7
protonated/deuterated
deuterated glucose in D2O
+0.1
+0.7
+0.7
+0.6
+0.5
protonated/deuterated
deuterated glucose in D2O
+0.1
+0.8
+0.8
+0.6
+0.5
protonated/deuterated
deuterated glucose in H2O
+0.2
+0.6
+1.0
+0.9
+0.8
protonated/deuterated
deuterated glucose in H2O
+0.7
+0.6
+1.0
+1.2
+0.8
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