Fitness for 5 genes in Escherichia coli BW25113

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500 ntfsr and ushA are separated by 217 nucleotidesushA and ybaK are separated by 36 nucleotidesybaK and sroB are separated by 121 nucleotidessroB and ybaP overlap by 2 nucleotides b0479: fsr - predicted fosmidomycin efflux system (NCBI), at 502,700 to 503,920 fsr b0480: ushA - UDP-sugar hydrolase (NCBI), at 504,138 to 505,790 ushA b0481: ybaK - hypothetical protein (NCBI), at 505,827 to 506,306 ybaK b4585: sroB - Novel sRNA, function unknown (RefSeq), at 506,428 to 506,511 sroB b0482: ybaP - hypothetical protein (NCBI), at 506,510 to 507,304 ybaP
Group Condition b0479 b0480 b0481 b4585 b0482
fsr ushA ybaK sroB ybaP
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.0 +0.4 -1.0 -6.8 -0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.1 -0.1 -0.7 -6.1 -0.4
nophagecontrol R1_N4_T1_phage0 -0.0 -0.4 -0.5 -6.2 -0.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.3 +0.1 -0.3 -6.8 +0.7
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.0 -0.2 -0.2 -5.6 -0.5
carbon source carbon source D-Mannose 0.5mM +0.3 -0.2 +0.5 -5.6 -0.1
stress octanol 0.08 vol% -0.3 -0.5 +0.3 -4.7 +0.4
carbon source Acetate (C) -0.2 +0.0 +0.3 -4.7 +0.1
stress methylglyoxal 0.008 vol% -0.0 -0.2 -0.9 -3.0 -0.0
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.0 +0.2 -0.4 -3.9 +0.1
motility inner cut, LB soft agar motility assay +0.2 +0.2 -0.8 -3.3 -0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.4 -0.3 -1.0 -2.6 -0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 +0.1 -0.6 -3.3 -0.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.3 -0.1 -1.4 -1.9 +0.0
phage N4_phage 0.009375 MOI TC1 -0.0 -0.5 +0.6 -3.5 -0.1
stress 3-methyl-2-butenol 1.25 vol% +0.2 -0.0 -0.4 -3.0 -0.1
phage Ste41 -0.3 -0.2 +0.5 -3.1 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.5 +0.3 -0.8 -3.2 +0.3
carbon source Ying_Others16 rep B; time point 1 +0.1 +0.0 -0.6 -2.5 +0.1
stress toluene 2.5 vol% +0.2 -0.3 -0.8 -1.8 +0.2
stress Chloramphenicol 0.005 mg/ml +0.4 -0.0 +0.0 -3.0 +0.2
stress Cisplatin 0.1 mg/ml +0.4 -0.7 -0.5 -1.7 +0.4
phage Ste41 +0.0 -0.2 +0.8 -1.9 -0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 -0.2 +0.4 -1.6 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.1 +1.6 -2.0 -0.0
carbon source carbon source D-Glucose 5mM +0.7 +0.1 -1.1 +1.8 +0.1
phage T7_phage 0.0015625 MOI TC2 +0.5 +0.5 -1.1 +2.3 -0.2
control PBS_cage_1_mice _Feces_22 hr -0.1 +0.6 +0.5 +2.3 -0.1
control PBS_cage_1_mice _Feces_16 hr -0.1 +0.7 +0.5 +2.6 -0.2
control PBS_cage_1_mice _Feces_40 hr -0.2 +0.4 +0.4 +2.9 +0.2
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