Fitness for 5 genes in Escherichia coli BW25113

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500 nthemH and aes overlap by 4 nucleotidesaes and gsk are separated by 151 nucleotidesgsk and ybaL are separated by 132 nucleotidesybaL and fsr are separated by 237 nucleotides b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197 aes b0477: gsk - inosine/guanosine kinase (NCBI), at 499,349 to 500,653 gsk b0478: ybaL - predicted transporter with NAD(P)-binding Rossmann-fold domain (NCBI), at 500,786 to 502,462 ybaL b0479: fsr - predicted fosmidomycin efflux system (NCBI), at 502,700 to 503,920 fsr
Group Condition b0475 b0476 b0477 b0478 b0479
hemH aes gsk ybaL fsr
control PBS_cage_1_mice _Feces_40 hr N.D. -0.7 +0.2 -0.3 -0.2
stress 2-Furfuraldehyde 0.25 vol% N.D. +0.1 -0.5 -0.8 +0.4
phage RB68 N.D. -0.4 -0.1 +0.4 -0.6
phage Ste41 N.D. -0.3 +0.3 -0.3 -0.3
motility inner cut, LB soft agar motility assay N.D. -0.5 -0.2 +0.3 -0.2
control PBS_cage_2_mouse_1_Cecum_40 hr N.D. -0.5 -0.3 +0.1 +0.3
carbon source carbon source D-Mannose 0.5mM N.D. -0.4 +0.3 -0.5 +0.3
drt_type_3 DRT_type_3 N.D. +0.3 -0.2 +0.2 -0.6
drt_type_3 DRT_type_3 N.D. +0.3 -0.2 +0.2 -0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. -0.2 +0.5 -0.3 -0.2
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM N.D. -0.5 +0.3 +0.2 -0.2
stress 3-methyl-1-butanol 1.25 vol% N.D. -0.2 +0.5 -0.2 -0.3
gfp_control GFP_control N.D. +0.4 -0.1 +0.1 -0.5
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. -0.1 +0.6 -0.3 -0.2
phage Δdbn_10 N.D. -0.5 +0.5 +0.2 -0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +0.3 +0.5 +0.2 -0.8
carbon source Dodecanol and Rhamnolipid (C) N.D. -0.1 +0.2 -0.3 +0.5
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. -0.5 +0.4 +0.5 -0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.5 +0.1 +0.5 +0.4
carbon source Dodecane and Rhamnolipid (C) N.D. -0.3 +0.2 +0.6 -0.1
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. +0.0 +1.2 -0.1 -0.4
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. +0.5 -0.2 +0.4 +0.1
phage T7_phage 0.00015625 MOI TC3 N.D. +0.3 +0.4 -0.2 +0.4
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM N.D. +0.0 +0.6 -0.1 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.9 +0.6 +0.5 +0.8
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. +0.0 +1.3 -0.1 -0.2
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.3 +0.5 +0.2 +0.2
carbon source carbon source D-Glucose 5mM N.D. +0.1 +0.2 +0.2 +0.7
phage T7_phage 0.00015625 MOI TC4 N.D. +0.1 +0.6 +0.5 +0.2
stress Cisplatin 0.1 mg/ml N.D. +0.1 +0.5 +0.3 +0.4
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