Fitness for 5 genes in Escherichia coli BW25113

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500 ntadk and hemH are separated by 235 nucleotideshemH and aes overlap by 4 nucleotidesaes and gsk are separated by 151 nucleotidesgsk and ybaL are separated by 132 nucleotides b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197 aes b0477: gsk - inosine/guanosine kinase (NCBI), at 499,349 to 500,653 gsk b0478: ybaL - predicted transporter with NAD(P)-binding Rossmann-fold domain (NCBI), at 500,786 to 502,462 ybaL
Group Condition b0474 b0475 b0476 b0477 b0478
adk hemH aes gsk ybaL
stress 2-Furfuraldehyde 0.25 vol% N.D. N.D. +0.1 -0.5 -0.8
control PBS_cage_1_mice _Feces_16 hr N.D. N.D. -0.4 -0.1 -0.5
stress Spectinomycin 0.0125 mg/ml N.D. N.D. -0.1 -0.5 -0.4
control PBS_cage_1_mice _Feces_40 hr N.D. N.D. -0.7 +0.2 -0.3
control PBS_cage_1_mice _Feces_22 hr N.D. N.D. -0.5 +0.1 -0.4
control PBS_cage_2_mouse_1_Cecum_40 hr N.D. N.D. -0.5 -0.3 +0.1
carbon source carbon source D-Mannose 0.5mM N.D. N.D. -0.4 +0.3 -0.5
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. N.D. -0.5 +0.3 -0.3
motility inner cut, LB soft agar motility assay N.D. N.D. -0.5 -0.2 +0.3
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. N.D. -0.2 +0.4 -0.3
phage RB68 N.D. N.D. -0.4 -0.1 +0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. N.D. -0.2 +0.5 -0.3
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM N.D. N.D. -0.5 +0.3 +0.2
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. N.D. -0.3 -0.2 +0.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. N.D. -0.5 +0.1 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -0.9 +0.6 +0.5
stress 3-methyl-1-butanol 1.25 vol% N.D. N.D. -0.2 +0.5 -0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM N.D. N.D. -0.1 +0.6 -0.3
phage Δdbn_10 N.D. N.D. -0.5 +0.5 +0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. -0.5 +0.4 +0.5
stress Aluminum chloride 5 mM N.D. N.D. +0.2 +0.5 -0.2
carbon source Dodecane and Rhamnolipid (C) N.D. N.D. -0.3 +0.2 +0.6
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB N.D. N.D. +0.5 -0.2 +0.4
stress LB Anaerobic with Ampicillin 0.00000195313 mM N.D. N.D. +0.3 +0.4 +0.2
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. +0.3 +0.5 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. N.D. +0.3 +0.5 +0.2
stress Cisplatin 0.1 mg/ml N.D. N.D. +0.1 +0.5 +0.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. +0.0 +1.2 -0.1
phage T7_phage 0.00015625 MOI TC4 N.D. N.D. +0.1 +0.6 +0.5
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. +0.0 +1.3 -0.1
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