Fitness for 5 genes in Escherichia coli BW25113

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500 ntpriC and ybaN are separated by 69 nucleotidesybaN and apt are separated by 152 nucleotidesapt and dnaX are separated by 128 nucleotidesdnaX and ybaB are separated by 52 nucleotides b0467: priC - primosomal replication protein N'' (NCBI), at 489,509 to 490,036 priC b0468: ybaN - conserved inner membrane protein (NCBI), at 490,106 to 490,483 ybaN b0469: apt - adenine phosphoribosyltransferase (NCBI), at 490,636 to 491,187 apt b0470: dnaX - DNA polymerase III subunits gamma and tau (NCBI), at 491,316 to 493,247 dnaX b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629 ybaB
Group Condition b0467 b0468 b0469 b0470 b0471
priC ybaN apt dnaX ybaB
stress octanol 0.08 vol% -0.1 -0.3 +0.0 N.D. -5.4
stress 2,4-Dinitrophenol 2 mM -0.5 -0.1 -0.3 N.D. -4.2
stress Nalidixic 0.006 mg/ml -1.2 -0.5 -0.2 N.D. -2.7
stress Ciprofloxacin Hydrochloride 0.0024 mM -1.1 -0.7 +0.0 N.D. -2.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.8 -0.2 -0.4 N.D. -4.1
stress 2,4-Dinitrophenol 2 mM -0.5 -0.2 -0.1 N.D. -3.0
stress Chlorite 1.25 mM -0.0 +0.5 -0.4 N.D. -3.7
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.0 -0.1 -0.6 N.D. -2.7
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 -0.1 -0.8 N.D. -2.6
stress LB Aerobic with Irgasan 0.0000003125 mM -1.1 -0.1 -0.6 N.D. -1.5
phage Lambda_ci1857 -0.1 -0.5 -0.8 N.D. -2.0
phage Δdbn_10 -0.1 +0.3 -0.4 N.D. -3.2
motility outer cut, LB soft agar motility assay -1.2 -0.1 -0.4 N.D. -1.5
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.4 -0.0 -0.7 N.D. -2.0
carbon source carbon source D-Mannose 5mM -2.6 -0.0 +0.5 N.D. -1.1
stress Cisplatin 0.1 mg/ml -0.0 +0.1 -0.3 N.D. -2.9
control PBS_cage_1_mice _Feces_40 hr -0.2 -0.6 -0.1 N.D. -2.2
carbon source carbon source D-Glucose 0.25mM -0.7 -0.1 -0.1 N.D. -2.2
stress Bacitracin 1 mg/ml +0.2 -0.4 +0.9 N.D. -3.6
phage R2_N4_T1_phage10 -0.5 -0.0 +0.7 N.D. -3.1
stress Cisplatin 0.1 mg/ml +0.2 +0.2 -0.2 N.D. -3.0
stress Cephalothin 0.01 mg/ml +0.6 +1.4 -0.3 N.D. -1.4
phage Ste41 +1.1 +0.0 +0.7 N.D. -1.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.3 +0.3 +0.5 N.D. -1.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.9 +0.6 +0.3 N.D. -2.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.9 +0.5 +0.9 N.D. -1.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.6 +0.9 -0.7 N.D. +1.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.7 +0.1 +1.2 N.D. -1.0
stress Trimethoprim 0.005 mM +0.9 -0.2 +0.0 N.D. +2.0
plasmid transformation vcDART vector transformation; agar assay +1.0 +0.3 +1.0 N.D. +0.9
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