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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
priC and ybaN are separated by 69 nucleotides
ybaN and apt are separated by 152 nucleotides
apt and dnaX are separated by 128 nucleotides
dnaX and ybaB are separated by 52 nucleotides
b0467: priC - primosomal replication protein N'' (NCBI), at 489,509 to 490,036
priC
b0468: ybaN - conserved inner membrane protein (NCBI), at 490,106 to 490,483
ybaN
b0469: apt - adenine phosphoribosyltransferase (NCBI), at 490,636 to 491,187
apt
b0470: dnaX - DNA polymerase III subunits gamma and tau (NCBI), at 491,316 to 493,247
dnaX
b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629
ybaB
Group
Condition
b0467
b0468
b0469
b0470
b0471
priC
ybaN
apt
dnaX
ybaB
stress
octanol 0.08 vol%
-0.1
-0.3
+0.0
N.D.
-5.4
stress
2,4-Dinitrophenol 2 mM
-0.5
-0.1
-0.3
N.D.
-4.2
stress
Nalidixic 0.006 mg/ml
-1.2
-0.5
-0.2
N.D.
-2.7
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-1.1
-0.7
+0.0
N.D.
-2.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.8
-0.2
-0.4
N.D.
-4.1
stress
2,4-Dinitrophenol 2 mM
-0.5
-0.2
-0.1
N.D.
-3.0
stress
Chlorite 1.25 mM
-0.0
+0.5
-0.4
N.D.
-3.7
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.0
-0.1
-0.6
N.D.
-2.7
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
-0.1
-0.8
N.D.
-2.6
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.1
-0.1
-0.6
N.D.
-1.5
phage
Lambda_ci1857
-0.1
-0.5
-0.8
N.D.
-2.0
phage
Δdbn_10
-0.1
+0.3
-0.4
N.D.
-3.2
motility
outer cut, LB soft agar motility assay
-1.2
-0.1
-0.4
N.D.
-1.5
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.4
-0.0
-0.7
N.D.
-2.0
carbon source
carbon source D-Mannose 5mM
-2.6
-0.0
+0.5
N.D.
-1.1
stress
Cisplatin 0.1 mg/ml
-0.0
+0.1
-0.3
N.D.
-2.9
control
PBS_cage_1_mice _Feces_40 hr
-0.2
-0.6
-0.1
N.D.
-2.2
carbon source
carbon source D-Glucose 0.25mM
-0.7
-0.1
-0.1
N.D.
-2.2
stress
Bacitracin 1 mg/ml
+0.2
-0.4
+0.9
N.D.
-3.6
phage
R2_N4_T1_phage10
-0.5
-0.0
+0.7
N.D.
-3.1
stress
Cisplatin 0.1 mg/ml
+0.2
+0.2
-0.2
N.D.
-3.0
stress
Cephalothin 0.01 mg/ml
+0.6
+1.4
-0.3
N.D.
-1.4
phage
Ste41
+1.1
+0.0
+0.7
N.D.
-1.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.3
+0.3
+0.5
N.D.
-1.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.9
+0.6
+0.3
N.D.
-2.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.9
+0.5
+0.9
N.D.
-1.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.6
+0.9
-0.7
N.D.
+1.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.7
+0.1
+1.2
N.D.
-1.0
stress
Trimethoprim 0.005 mM
+0.9
-0.2
+0.0
N.D.
+2.0
plasmid transformation
vcDART vector transformation; agar assay
+1.0
+0.3
+1.0
N.D.
+0.9
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